PCR Equipment and Supplies

A wide variety of products designed for use in various PCR procedures. Includes thermal cyclers, oligonucleotides, dNTPs, enzymes, assays, arrays, master mixes, reagents, kits, etc.

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106 – 120 of 8,347 results

106

Applied Biosystems™ MeltDoctor™ HRM Master Mix

Contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Form	Liquid
For Use With (Application)	Genotyping
PCR Method	qPCR
Detection Method	HRM
Polymerase	AmpliTaq Gold 360 DNA Polymerase
Genotyping Target	SNPs (Unknown or Numerous), Epigenetic Targets
Product Type	HRM Master Mix
For Use With (Equipment)	7500 Fast System, 7900HT Fast System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast
Label or Dye	High Resolution Melting (HRM),SYTO 9
Technique	HRM (High Resolution Melting) Analysis, Mutation Scanning
Product Line	MeltDoctor

107

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Invitrogen™ Platinum™ PCR SuperMix High Fidelity

Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
Polymerase	Platinum Taq DNA Polymerase High Fidelity
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	6X
Size (Final Product)	15 kb or less
Reaction Format	SuperMix or Master Mix
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Overhang	Mixed
Concentration	1.1X
For Use With (Application)	Hot-start PCR,High-fidelity PCR

108

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Invitrogen™ Template Switching Reverse Transcription Oligonucleotides

Use this set of oligonucleotides for template switching cDNA synthesis and preamplification. Template Switching Oligonucleotide and Capturing Oligo (dT) are used to incorporate adaptor sequences to full-length cDNA. Preamplification primer enables PCR amplification of obtained cDNA.

Pricing & Availability
Specifications

Color	Colorless
Label or Dye	No
Quantity	48 Reactions

109

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Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit

The SuperScript IV CellsDirect cDNA Synthesis Kit is optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA. Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in PCR and qPCR.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Format	Kit
Reverse Transcriptase	SuperScript IV
Reaction Format	Premixed Components
Final Product Type	First-Strand cDNA
Reaction Speed	10 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	50°C
Technique	Reverse Transcription
Starting Material	Cells
For Use With (Application)	RT-PCR,Real Time PCR (qPCR)

110

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Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript III
Final Product Type	First-Strand cDNA
For Use With (Application)	Real Time PCR (qPCR)
Detection Method	qPCR
Format	Kit
Size (Final Product)	Up to 10 kb
Reaction Format	Master Mix
Reaction Speed	60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	42°C
Technique	Reverse Transcription
Starting Material	RNA

111

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Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase

AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
Polymerase	AccuPrime GC-Rich DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2X
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Overhang	Blunt
For Use With (Application)	Hot-start PCR

112

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Applied Biosystems™ TaqMan™ Universal Master Mix II, with UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Content And Storage	Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq)	2 X
Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Product Type	Universal Master Mix II
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
Product Line	TaqMan

113

Applied Biosystems™ SYBR™ Select Master Mix for CFX

A cost-effective solution for real-time PCR applications.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Purity or Quality Grade	UP (Ultra Pure)
Form	Liquid
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Detection Method	SYBR
Polymerase	AmpliTaq DNA Polymerase
Format	Vial
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	AB 7900HT, Standard Mode, AB StepOne™, Fast Mode, AB 7500, Standard Mode, AB 7500, Fast Mode, Bio-Rad CFX96 Touch™, AB StepOnePlus™, Fast Mode, AB 7900HT, Fast Mode, AB StepOne™, Standard Mode, Bio-Rad CFX Connect™, AB 7900 HT 384-Well System, Bio-Rad CFX384 Touch™, AB StepOnePlus™, Standard Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast or Standard
Label or Dye	SYBR GreenER
Hot Start	Built-In Hot Start
Product Line	SYBR

114

Invitrogen™ Cells-to-Signal™ Lysis Buffer (10 ml Bottle)

The same lysis buffer found in the Invitrogen Cells-to-Signal™ Kit. Supplied in five 10 mL bottles.

Pricing & Availability

115

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Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl₂

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

Pricing & Availability
Specifications

Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Product Type	DNA Polymerase
Hot Start	Built-In Hot Start
Overhang	3'-A
Concentration	5 U/μL
Exonuclease Activity	5' - 3'

116

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Invitrogen™ PCR_X Enhancer System

PCR_x Enhancer System is used in conjunction with DNA polymerases to optimize PCR of problematic and/or GC-rich templates.

Pricing & Availability

117

Thermo Scientific™ MgCl₂ (magnesium chloride) (25 mM)

Thermo Scientific PCR reagents meet high quality standards to generate accurate results from even the most challenging molecular biology applications.

Pricing & Availability

118

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Applied Biosystems™ Mouse ACTB (Actin, Beta) Endogenous Control (FAM™ Dye/MGB probe, Non-Primer Limited)

Mouse ACTB (Actin, Beta) Endogenous Control (FAM™ Dye/MGB probe, Non-Primer Limited)

Pricing & Availability

119

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Applied Biosystems™ Human HPRT1 (HGPRT) Endogenous Control (VIC™/MGB probe, primer limited)

Human HPRT1 (HGPRT) Endogenous Control (VIC™/MGB probe, primer limited)

Pricing & Availability

120

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Applied Biosystems™ TaqMan™ RNase P Control Reagents Kit

TaqMan™ RNase P Control Reagents Kit

Pricing & Availability

PCR Equipment and Supplies

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Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Applied Biosystems™ TaqMan™ Universal Master Mix II, with UNG GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2 GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Applied Biosystems™ Mouse ACTB (Actin, Beta) Endogenous Control (FAM™ Dye/MGB probe, Non-Primer Limited) Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Applied Biosystems™ Human HPRT1 (HGPRT) Endogenous Control (VIC™/MGB probe, primer limited) Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit

Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit

Applied Biosystems™ TaqMan™ Universal Master Mix II, with UNG

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl₂

Applied Biosystems™ Mouse ACTB (Actin, Beta) Endogenous Control (FAM™ Dye/MGB probe, Non-Primer Limited)

Applied Biosystems™ Human HPRT1 (HGPRT) Endogenous Control (VIC™/MGB probe, primer limited)