DDB1 protein is a recombinant human DNA damage-binding protein 1 produced in Sf9 insect cells and supplied as an N-terminal His-tagged, 0.22 μm-filtered solution formulated for biochemical and cell-based research. The preparation is characterized for concentration, purity, molecular weight, endotoxin, and storage stability to support studies of DNA repair, ubiquitination, and protein-protein interactions.
- Recombinant human DDB1 expressed in Sf9 insect cells.
- N-terminal His tag for affinity purification and detection.
- Supplied in 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
- High purity (>90% by reducing SDS-PAGE) suitable for biochemical assays.
- Provided at 1.45 mg/mL to facilitate assay setup.
- Low endotoxin level (<1 EU/μg) for sensitive applications.
- Store at -80°C; stable short term at -20°C; avoid repeated freeze-thaw cycles.