Oligonucleotides
New England Biolabs, Inc. Monarch HC DNA Cleanup 10 p
The Monarch Spin High-Capacity DNA Cleanup Kit (100 ug) offers higher DNA cleanup capacities and yields compared to our Monarch PCR DNA Cleanup Kit (5 ug) while maintaining the convenient spin column format for silica membrane-based DNA cleanup This high-capacity version efficiently purifies and concentrates up to 100 ug of high-quality double-stranded and single-stranded DNA from enzymatic reactions such as PCR restriction digestion ligation and reverse transcription The kit features precision-engineered spin columns uniquely designed to enable low elution volumes as little as 50 ul resulting in highly concentrated DNA suitable for downstream applications The spin columns are compatible with microcentrifuges centrifuges and vacuum manifolds without additional plastic accessories The optimized buffer system helps ensure efficient DNA binding and purification without the need to adjust pH
Invivogen Negative control for ODN M362, 200 µg
ODN M362 Control contains GpC dinucleotides instead of CpGs and can be used as a negative control for ODN M362 (type C specific for human / mouse TLR9).
New England Biolabs, Inc. Monarch® Spin Plasmid Miniprep Kit - 50 preps
The Monarch Spin Plasmid Miniprep kit, a newer version of our original Monarch Miniprep kit, offers a unique method for plasmid preparation and purification, enabling extraction of highly concentrated plasmid DNA from various bacterial strains, with yields of up to 20 ug of high-quality DNA. This kit features upgraded spin columns precision-engineered to uniquely allow for low elution, in as little as 30 ul, for highly concentrated DNA in downstream application such as sequencing, restriction digestion, transformation, transfection, and more. A colored buffer system helps indicate completion of key lysis and neutralization steps for an easy to follow workflow and shorter, faster protocols allow for reduced hands-on time and less spin time. Compared to leading suppliers, these kits use significantly less plastic through uniquely designed columns and bottles with plastic reduction while still maintaining structural integrity for centrifugation or vacuum manifolds and other steps
INTACT GENOMICS INC igScript™ Glycerol-free Reverse Transcriptase 10,000 units (200 u/µl)
igScript™ Glycerol-free Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. igScript RT is an RNA-directed DNA polymerase which lacks 3´ → 5´ exonuclease activity and is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR analysis and other applications.Benefits: Recombinant MMLV reverse transcriptase with greatly reduced RNase H activity. Active at temperatures up to 55°C. Highly efficient at producing full-length cDNA from as little as 50 pg of total RNA.Product Includes: igScript™ Glycerol-free Reverse Transcriptase 10x igScript™ RT Reaction BufferStorage Temperature: –80 °C
INTACT GENOMICS INC igScript™ Probe-Based One Step RT-qPCR Kit 1000 Reactions
IgScript™ probe-based RT-qPCR kit combines i) IgScript™ first strand cDNA synthesis kit and ii) probe-based qPCR 2x master mix for high efficiency, wide dynamic range, sensitivity, and specificity. The cDNA kit includes 5x IgScript™ master mix (Reverse Transcriptase, RNase inhibitor, dNTPs, buffer, MgCl₂, stabilizers), plus oligo-dT [d(T)23VN] and random hexamer primers for complete coverage of mRNA and non-polyadenylated RNAs. IgScript™ Reverse Transcriptase is a recombinant MMLV enzyme with reduced RNase H activity and higher thermostability, enabling full-length cDNA synthesis at 42–50 ºC. The qPCR 2x master mix (excluding primers/template) contains proprietary buffer, hot start Taq polymerase, low ROX dye, MgCl₂, dNTPs, stabilizers, and enhancers for robust amplification.Applications: Gene expression validation, multiplexing, mutation detection, pathogen/viral detection, GMO characterization, profiling.
Revvity Health Sciences Inc NEXTFLEX⢠Unique dual index barcodes (10NT, 577-672)
NEXTFLEX⢠Unique dual index barcodes (10NT, 577-672)
AMPLYUS LLC miniPCR bio eDNA Project: Sampling Soil for Antibiotic Resistance
Join a nationwide monitoring program that tests for antibiotic resistance genes in environmental soil samples. Students collect soil samples based on their own hypotheses about antibiotic resistance hotspots, extract total environmental DNA, and use PCR to detect tetracycline resistance genes.
Sino Biological Human GAPDH qPCR Primer Pair
Verified forward and reverse primers for analyzing the quantitative expression of gene.
New England Biolabs, Inc. E. coli tRNA - 1 mg
E. coli tRNA is a mix of tRNAs isolated from E. coli and purified by chromatography to remove contaminating proteins, including nucleases, as well as other nucleic acids. This tRNA formulation sustains translation in E. coli-based in vitro protein synthesis reactions (also referred to as cell-free protein synthesis or CFPS). It is also suited for molecular biology applications that require highly pure and active prokaryotic tRNA.
- Highly active tRNA for in vitro translation
- Purified by chromatography, no organic solvents
- Highly pure with undetectable levels of nucleases and proteases
- Manufactured with reduced environmental impact
- Compatible with a variety of molecular biology workflows
Revvity Health Sciences Inc NEXTFLEX⢠Unique dual index barcodes (10NT, 385-480)
NEXTFLEX⢠Unique dual index barcodes (10NT, 385-480)