VAZYME SupRealQ Ultra Hunter SYBR qPCR Master Mix (U+) is a specialized premix for qPCR reactions using the SYBR Green I fluorescence method, with a purple color to facilitate sample loading. The core enzyme is a Taq polymerase selected through BioSmart platform-directed screening, featuring strong 3' end mismatch recognition and high specificity. It is combined with high-closure dual-species antibodies to form a hot-start Taq enzyme, which maintains strict closure at 55°C. Paired with an optimally formulated buffer for qPCR, it enables precise detection and efficient amplification of target genes, even with low template amounts or low-expression genes. The reagent includes a dUTP/UDG contamination prevention system that works at room temperature, preventing aerosol contamination and ensuring the accuracy of qPCR results.