High Fidelity PCR Reagents and Kits
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Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Standalone |
| Product Type | High-Fidelity DNA Polymerase |
| Reaction Speed | Fast |
| Hot Start | No |
| Overhang | Blunt |
| Concentration | 2 U/μL |
| For Use With (Application) | Standard PCR,High-fidelity PCR |
Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix
Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion Flash II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 25X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | High-Fidelity PCR Master Mix |
| Reaction Speed | Fast |
| Color | Colorless |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion High-Fidelity DNA Polymerase |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | High-Fidelity PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | No |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Standard PCR,High-fidelity PCR |
Thermo Scientific™ Phusion™ Plus DNA Polymerase
Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion Plus DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >100X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Separate components |
| Reaction Speed | 15 to 30 sec./kb |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion™ Plus PCR Master Mixes
Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion Plus DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >100X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Ready to use master mix |
| Reaction Speed | 15 to 30 sec./kb |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion Hot Start II High-Fidelity PCR Master Mixes
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion Hot Start II High-Fidelity DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Hot Start High-Fidelity PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion Site-Directed Mutagenesis Kit
Generate mutations with this fast and reliable system.
Invitrogen™ Platinum™ SuperFi II PCR Master Mixes
Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ Platinum™ SuperFi™ PCR Master Mix
Invitrogen Platinum SuperFi PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ Platinum™ SuperFi™ DNA Polymerase
Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Separate Components |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ Platinum™ SuperFi II DNA Polymerase
Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Standalone |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion™ Hot Start II DNA Polymerase & dNTP Mix (10 mM each)
This package includes Phusion Hot Start II High-Fidelity DNA Polymerase (4 x 500 U) and high quality dNTP Mix (10 mM each, 2 x 1 mL).
Thermo Scientific™ Phusion High-Fidelity PCR Kit
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion High-Fidelity DNA Polymerase |
| GC-Rich PCR Performance | High |
| Content And Storage | • Phusion DNA Polymerase (2 U/μL) • 5X Phusion GC & HF Buffer • dNTP mix (10 mM each) • Control template (λ DNA) and DNA size standard • 1.3 kb & 10 kb primers (4 μM each) • 50 mM MgCl2 solution and DMSO Store at –20°C. |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Separate Components |
| Product Type | High-Fidelity PCR Kit |
| Reaction Speed | Fast |
| Hot Start | No |
| Overhang | Blunt |
| For Use With (Application) | Standard PCR,High-fidelity PCR |
Thermo Scientific™ Phusion™ Hot Start II DNA Polymerases (2 U/μL)
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Standalone |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2 U/μL |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion U Hot Start DNA Polymerases
Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion U Hot Start DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 25X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Standalone |
| Product Type | Hot Start DNA Polymerase |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2 U/μL |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |