Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Norgen Biotek Corp 2X One-Step Rt-Pcr Mas 100Rxns
Norgen’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, and a PCR enhancer/stabilizer. The user needs only to add the template, the primer set and water to the Master Mix to set up the RT-PCR reaction. This convenient 2X One-Step RT-PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of RNA templates with high yields of PCR products.Taq Source: An E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1RT Source: An E. coli strain with a cloned reverse transcriptase gene from M-MuLV.
Cytek Biosciences Inc FastPROBE™ qPCR Lo-ROX Master Mix
FastPROBE™ qPCR Lo-ROX Master Mix ..... Cat. No. 31-5111-0100R ..... Size 100 rxns
New England Biolabs, Inc. Luna® Universal One-Step RT-qPCR Kit – 2500 reactions
In the Luna One-Step RT-qPCR Kit, Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling, providing added security for setting up reactions at room temperature. The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. Note that to ensure full activation of the WarmStart Luna RT, incubation at temperatures lower than 50°C is not recommended.
- Non-interfering, visible tracking dye helps to eliminate pipetting errors
Cytek Biosciences Inc CYBRFast™ 1-Step RT-qPCR Hi-ROX Kit
CYBRFast™ 1-Step RT-qPCR Hi-ROX Kit ..... Cat. No. 31-5202-0300R ..... Size 300 rxns
Integrated DNA Technologies Inc PrimeTime Gene Expression Master Mix, High PCR Efficiency >90%, Compatible With Various qPCR Systems, 5 mL
The PrimeTime Gene Expression Master Mix is a 2X concentrated solution that enhances accuracy and efficiency in qPCR. It guarantees PCR efficiencies over 90% and R² values above 0.99, making it reliable for gene expression analysis. The mix features exceptional stability at room temperature and high temperatures, reducing shipping costs and environmental impact. Its broad compatibility with various primers and probes differentiates it in the market, perfect for both routine and high-throughput applications.
Zymo Research Corporation ZymoTaq qPCR Premix (50 Rxns)
It features a hot-start DNA polymerase and buffer system optimized for the amplification of bisulfite-treatedDNA. It also includes an intense dsDNA-specific fluorescentdye, SYTO 9, for sensitive real-time DNA quantification. This technology can also be found in the Femto Quantification Kits (Cat. No. E2005, E2006, E2007) for low copy detection of DNA from human, bacteria, or fungi.
Eagle Biosciences Inc Plasmid Residual DNA Detection Kit (qPCR)
Plasmid Residual DNA Detection Kit (qPCR)
NEW ENGLAND BIOGROUP LUNA-FL automated cell counter fluorescent calibration beads. 1 pk/50 tests. .5 mL.
LUNA-FL automated cell counter fluorescent calibration beads. 1 pk/50 tests. .5 mL.
TRIBIOSCIENCE INC Porcine QPCR, 100 reactions
Tribioscience’s Porcine QPCR Kit is designed for the target-DNA specific detection of porcine derived in food, animal feed ingredients, and other samples. The assay uses a real-time PCR-based protocol with fluorescent-probe to detect target DNA. It can detect as few as 10 copies of the target DNA in a reaction and it exhibits high specificity for Porcine. No cross reactivity is observed with other animal species. The detection of target DNA confirms ingredient authenticity or prevents food fraud, ethical issues, or health concerns.
Active Motif ChIP-IT qPCR Analysis Kit
The ChIP-IT qPCR Analysis Kit was designed to simplify ChIP qPCR analysis and data interpretation. The kit includes standard curve DNA, a standard curve primer pair, positive and negative control qPCR primer sets for human and mouse samples and an analysis spreadsheet that automatically normalizes and graphs the data. The normalization calculations take into consideration the amount of chromatin that was used in the ChIP reaction, the resuspension volume of the ChIP DNA and the primer efficiencies to introduce a level of consistency in the data output that enables a reliable comparison of many samples across multiple experiments. Based on the consistency of the data output, Active Motif is able to make specific recommendations in regards to data interpretation so that customers can reliably assess the quality of their ChIP DNA.
Alkali Scientific Radiant™ Green Lo-ROX qPCR Kit, 2,000 Reactions, (20 x 1mL)
Alkali Scientific offers Radiant™ Green Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Green Lo-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.