Dissociation Reagents
Revvity Health Sciences Inc AlphaLISA Human CCL22 Detection Kit, 100 Assay Points
AlphaLISA Human CCL22 Detection Kit, 100 Assay Points
AllCells LLC Peripheral Blood, Cryopreserved, CD4+ Helper T Cells, Negatively Selected, 25M
CD4+ Helper T cells are negatively selected from peripheral blood leukapheresis using immunomagnetic microbeads targeting non-helper T cells. CD4+ Helper T cells are shipped in a 2mL cryovial. Purity is determined prior to cryopreservation and is ≥ 85%. Viability is ≥ 80% post-thaw.
Amsbio LLC Detachin Cell Detachment Solution
Detachin™ Cell Detachment Solution is a superior alternative to Trypsin/EDTA for gently detaching adherent cells from in vitro growth vessels. Detachin™ provides quick, gentle, and effective detachment of a wide variety of adherent cells, including primary cells, from all known tissue culture plastic ware. It contains protease and collagenase activities in an isotonic, phosphate buffer solution with EDTA.
Cayman Chemical TrypsIn InhIbItrsoyben 100mg
An inhibitor of serine proteases that dose-dependently inhibits trypsin from a variety of sources, as well as plasma kallikrein, thrombin, plasmin, and other serine proteases; partially inhibits chymotrypsin and neutrophil elastase
Alkali Scientific CellPro™ Trypsin-EDTA Solution 0.25% in HBSS, w/o Ca/Mg, 100 mL, 10mL, 10/Cs
This perfect blend of trypsin (0.25%) and EDTA (0.53mM) is combined in calcium-free and magnesium-free Hanks Balanced Salt Solution along with phenol red. It has been shown to be a safe and effective means of dissociating a wide variety of cells in culture. Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. Triple 0.1µm sterile-filtered Porcine parvovirus screened Ships on ice. Store frozen at -20 to -10°C
STEMCELL Technologies Collagenase/Hyaluronidase, Size: 10 mL
10X Collagenase/Hyaluronidase in Dulbecco’s Modified Eagle’s Medium (DMEM) for the enzymatic dissociation of human mammary tissue.
Sigma Aldrich Fine Chemicals Biosciences Angiotensin Converting Enz
The gene for angiotensin converting enzyme-2 (ACE-2) is mapped to human chromosome Xp22.2. This protein is the first known human homologue of ACE. ACE2 has been identified from 5AE sequencing of a human heart failure ventricle cDNA library. It contains a signal peptide one metalloprotease active site and a transmembrane domain. ACE-2 is a secreted and an integral membrane protein. It is expressed predominantly on the endothelium.
Sigma Aldrich Fine Chemicals Biosciences Trypsin Inhibitor from soy
Soybean trypsin inhibitor (STI) comprises 181 amino acids. It is a monomeric protein corresponding to a molecular weight of 20 kDa and has two disulfide bridges. It belongs to serpins A serine protease inhibitors family.
Sigma Aldrich Fine Chemicals Biosciences Cell Dissociation Solution
Cell dissociation by non-enzymatic methods in human mesenchymal stromal cells aid in their detachment and preserves their functionality especially the expression of cell surface chemokine receptor.