Perform hybridoma selection and cloning in 1 step with ClonaCell™-HY Medium D. Using this methylcellulose-based medium, easily isolate individual clones with a high probability of monoclonality for further screening and expansion. Hybridoma clones typically have a wide range of growth rates and productivities; when cloned using this product, they form visible and discrete monoclonal colonies, preventing the loss of rare and high-producing clones due to overgrowth. The hybridoma colonies can be easily picked from the semi-solid medium by manual or robotic methods and dispersed into a liquid medium for screening and expansion.