STEMdiff™ Kidney Organoid Kit is a complete, serum-free cell culture medium system that supports highly efficient and reproducible generation of human pluripotent stem cell (hPSC)-derived kidney organoids in a simple, two-stage differentiation protocol. These kidney organoids are composed of podocytes, proximal tubules, distal tubules, as well as the associated endothelium and mesenchyme. Kidney organoids generated with STEMdiff™ Kidney Organoid Kit are tested for compatibility with phenotypic high-throughput assays such as nephrotoxic compound screening. They also provide a relevant and convenient model for studies related to developmental biology and disease modeling.