Additional Selective and Differential Cell Culture Media
SCIENCELL RESEARCH LABORATORIES INC HUMAN HEPATOCYTES MEDIA ONLY
NC2024153 HUMAN HEPATOCYTES MEDIA ONLY
ILEX LIFE SCIENCES LLC PANCOLL W. HEPES 1.077 6X500ML
PAN-Biotech Pancoll Human: Cell Separating Solution, w: 10 mM HEPES, Density 1.077 g/ml (6 x 500 ml). MANUFACTURER: PAN-Biotech GmbH (Germany). STORAGE: +2°C-RT. DENSITY: 1.077 g/ml. EDTA: Without EDTA. HEPES: With 10 mM HEPES. STERILE: Yes. COMPOSITION: Polysucrose: ~ 57,000.00 mg/L; Diatrizoic Acid: ~ 86,900.00 mg/L. DESCRIPTION: In many cases the isolation of cells is the first step for gene expression studies or in diagnostic procedures. Besides biological separation techniques physical separation methods are most commonly used. These methods use physical differences such as size and weight of the particles to be separated. For this purpose so-called separation solutions (also known as centrifugation media or density gradient media) are used. PAN-Biotech offers a variety of ready-to-use products with a density of 1.063 g/ml up to 1.119 g/ml for a very wide range of cell isolation applications.
NEOGEN Yeast Extract, 10 kg
Yeast Extract is an autolysate of yeast cells used in preparing microbiological culture media in a laboratory setting. Yeast Extract is not intended for use in the diagnosis of disease or other conditions in humans.Yeast Extract is the water-soluble portion of autolyzed yeast. The autolysis is carefully controlled to preserve naturally occurring B-complex vitamins. Yeast Extract is prepared and standardized for bacteriological use and cell cultures and is an excellent stimulator of bacterial growth. Yeast Extract is generally employed in the concentration of 0.3% - 0.5%. Yeast Extract is typically prepared by growing baker’s yeast, Saccharomyces spp., in a carbohydrate-rich plant medium. The yeast is harvested, washed, and resuspended in water, where it undergoes autolysis, i.e., self-digestion using the yeast’s enzymes.For more information visit https://www.neogen.com/solutions/Microbiology/yeast-extract
Promocell GmbH PromoCell Melanocyte Basal Medium 500 ml
PromoCell Melanocyte Growth Medium is a PMA (Phorbol Myristate Acetate)-containing Medium and has been developed for the in vitro cultivation of juvenile melanocytes. Since PMA is a tumor promoting mitogen, it may interfere with some experimental approaches. In these cases, we recommend using the PMA-free Melanocyte Growth Medium M3.While the supplements of the Melanocyte Growth Medium are serum-free, the Basal Medium is manufactured with 0.2% serum.| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.5 μg / ml || Phorbol Myristate Acetate | 10 ng / ml |
Promocell GmbH PromoCell Myocyte Basal Medium 500 ml
The PromoCell Myocyte Growth Medium is a low serum (5% V/V) medium supplemented with well chosen hormones and growth factors. It was developed for the optimal expansion and maintenance of primary cardiac myocytes in culture.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for murine and primary rat cardiac myocytes.| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.05 ml / ml || Epidermal Growth Factor (recombinant human) | 0.5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 2 ng / ml || Insulin (recombinant human) | 5 µg / ml |
Cambridge Isotope Laboratories Celtone plus base powder (unlabeled) 1 g
Celtone plus base powder (unlabeled) 1 g
NEOGEN LB Agar, Miller, 5 kg
LB Agar (Miller), a nutritionally rich medium designed for growth of pure cultures of recombinant strains, is based on the formulation described by Miller. E. coli is grown to late log phase in LB Medium. Some plasmid vectors replicate to a high copy number and do not require selective amplification. Some vectors do not replicate so freely and need to be selectively amplified. Chloramphenicol may be added to inhibit host synthesis and prevent replication of the bacterial chromosome.LB Agar, Miller contains 10 g/L of sodium chloride, different from the levels in Lennox formulations. This allows the researcher to select the optimal salt concentration for a specific strain. The medium may be aseptically supplemented with glucose or antibiotics.For more information visit https://www.neogen.com/solutions/Microbiology/lb-agar-miller
NEOGEN Brilliant Green Agar with Sulfapyridine, 500 g
Brilliant Green Agar w/ Sulfapyridine is used for the selective enrichment of Salmonella spp. in a laboratory setting. Brilliant Green Agar w/ Sulfapyridine is not intended for use in the diagnosis of disease or other conditions in humans.Brilliant Green Agar was first described by Kristensen et al and later modified by Kauffmann. The outstanding selectivity of this medium permits the use of moderately heavy inocula evenly distributed over the surface. The addition of sulfonamides into Brilliant Green Agar further inhibits Escherichia coli and Proteus spp. Osborne and Stokes used 0.1% Sodium Sulfapyridine to enhance the recovery of Salmonella from whole egg and egg yolk.For more information visit https://www.neogen.com/solutions/Microbiology/brilliant-green-agar-with-sulfapyridine
NEOGEN Tryptose, 5 kg
Tryptose is an enzymatic digest of protein for use in preparing microbiological culture media in a laboratory setting. Tryptose is not intended for use in the diagnosis of disease or other conditions in humans. Tryptose was developed while studying growth requirements of Brucella spp. Huddleson found Tryptose media to be equal or superior to meat infusion media, providing uniformity for the cultivation and differentiation of fastidious organisms including Neisseria gonorrhoeae, Streptococcus milleri and Brucella spp.. Casman reported that a medium consisting of 2% Tryptose, along with other media ingredients, equaled fresh beef infusion base with respect to organism growth. Tryptose is recommended in broth and agar formulations, and when used in media containing blood to permit hemolytic reactions. Tryptose is used in the preparation of Tryptose Broth (Product Code No. NCM0087).For more information visit https://www.neogen.com/solutions/Microbiology/tryptose
NEOGEN Salmonella Shigella (SS) Agar, 10 kg
Salmonella Shigella (SS) Agar is used for the isolation of Salmonella spp. and some strains of Shigella spp. Salmonella Shigella (SS) Agar is not intended for use in the diagnosis of disease or other conditions in humans. Salmonella Shigella (SS) Agar is a modification of the Desoxycholate Citrate Agar described by Leifson. Salmonella Shigella Agar is superior to a number of other media for the isolation of Salmonella spp. and Shigella spp. Salmonella Shigella (SS) Agar is recommended for testing food samples for the presence of Salmonella spp. and some Shigella spp. Formula:Beef Extract - 5.0 g/LEnzymatic Digest of Casein - 2.5 g/LEnzymatic Digest of Animal Tissue - 2.5 g/LLactose - 10.0 g/LBile Salts - 8.5 g/LSodium Citrate - 8.5 g/LSodium Thiosulfate - 8.5 g/LFerric Citrate - 1.0 g/LBrilliant Green - 0.00033 g/LNeutral Red - 0.025 g/L Agar - 13.5 g/L For more information visit https://www.neogen.com/solutions/Microbiology/salmonella-shigella-agar
NEOGEN Baird-Parker Agar, 10 kg
Baird-Parker Agar is used for detection and enumeration of Staphylococcus aureus in foods and is not intended for use in the diagnosis of disease or other conditions in humans.Baird-Parker Agar was first described in 1962. It is a selective medium for the isolation and presumptive identification of coagulase-positive staphylococci. This medium is used extensively for detecting Staphylococcus aureus in foods, dairy products, and other materials. Coagulase-positive staphylococci can grow and reproduce in cosmetic products. These products are tested for the presence of coagulase-positive staphylococci using standard microbiological methods.Formula:Enzymatic Digest of Casein - 10.0 g/LBeef Extract - 5.0 g/LYeast Extract - 1.0 g/LLithium Chloride - 5.0 g/LGlycine - 12.0 g/LSodium Pyruvate - 10.0 g/LAgar - 17.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/baird-parker-agar
NEOGEN DRBC Agar (BAM), 500 g
DRBC Agar (BAM) is used for the selective isolation and enumeration of yeasts and molds from foods in a laboratory setting. DRBC Agar is not intended for use in the diagnosis of disease or other conditions in humans.DRBC Agar (BAM) is based on Dichloran Rose Bengal Chlortetracycline (DRBC) Agar formula described by King, Hocking, and Pitt. DRBC Agar conforms with APHA guidelines for the mycological examination of foods containing chloramphenicol rather than chlortetracycline as proposed by King, Hocking, and Pitt. DRBC Agar is a selective medium, supporting good growth of yeasts and molds.For more information visit https://www.neogen.com/solutions/Microbiology/drbc-agar-bam
NEOGEN Vogel Johnson Agar, 500 g
Vogel Johnson Agar is used for the isolation of staphylococci in a laboratory setting. Vogel Johnson Agar is not intended for use in the diagnosis of disease or other conditions in humans. Coagulase-positive staphylococci, primarily Staphylococcus aureus, are among the microorganisms that cause spoilage or chemical changes in cosmetic products. To isolate coagulase-positive, mannitol fermenting staphylococci, Vogel and Johnson modified Tellurite-Glycine Agar by Zebovitz, Evans, and Niven. The modification included increasing mannitol and adding a pH indicator. Vogel Johnson Agar selects and differentiates coagulase-positive staphylococci that ferment mannitol and reduce tellurite. Vogel Johnson (VJ) Agar is specified in standard methods testing for cosmetics and nutritional supplements.For more information visit https://www.neogen.com/solutions/Microbiology/vogel-johnson-agar
NEOGEN Modified Semi-Solid Rappaport-Vassiliadis (MSRV) Agar, 5 kg
Modified Semi-Solid Rappaport-Vassiliadis (MSRV) Agar is used with Novobiocin for the rapid detection of motile Salmonella spp. and is not intended for use in the diagnosis of disease or other conditions in humans. MSRV was developed in 1986 by De Smedt, Bolderdijk and Rappold as a rapid means of Salmonella detection. The medium, based upon Rappaport Vassiliadis broth, is inoculated directly from the preenrichment medium, in the center of the plate. Motile organisms spread from the center in the semi-solid agar, but non-salmonellas are inhibited by the selective agents. After overnight incubation the use of polyvalent salmonella antisera or a latex kit can confirm the presence of a Salmonella.For more information visit https://www.neogen.com/solutions/Microbiology/modified-semi-solid-rappaport-vassiliadis-agar
NEOGEN Buffered Listeria Enrichment Broth, 10 kg
Buffered Listeria Enrichment Broth is used for selective enrichment of Listeria spp and is not intended for use in the diagnosis of disease or other conditions in humans.Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. Evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey, frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage, and river water.For more information visit https://www.neogen.com/solutions/Microbiology/buffered-listeria-enrichment-broth