Additional Selective and Differential Cell Culture Media
NEOGEN m-Endo Agar, 500 g
m-Endo Agar is used for the enumeration of coliforms in water by the membrane filtration method in a laboratory setting. m-Endo Agar is not intended for use in the diagnosis of disease or other conditions in humans.The coliform group, especially Escherichia coli, are used as indicators of fecal pollution in water, for assessing the effectiveness of water treatment and disinfection, and for monitoring water quality. m-Endo Agar is prepared according to the formula of McCarthy, Delaney, and Grasso, and used in testing water for coliforms by a two-step membrane filtration procedure. Lauryl Tryptose Broth is used as the preliminary enrichment, resulting in higher coliform counts.The American Public Health Association (APHA) recommends m-Endo Agar in standard total coliform membrane filtration procedure for testing water, wastewater, and foods.For more information visit https://www.neogen.com/solutions/Microbiology/m-endo-agar
NEOGEN Brilliant Green Agar with Sulfapyridine, 5 kg
Brilliant Green Agar w/ Sulfapyridine is used for the selective enrichment of Salmonella spp. in a laboratory setting. Brilliant Green Agar w/ Sulfapyridine is not intended for use in the diagnosis of disease or other conditions in humans.Brilliant Green Agar was first described by Kristensen et al and later modified by Kauffmann. The outstanding selectivity of this medium permits the use of moderately heavy inocula evenly distributed over the surface. The addition of sulfonamides into Brilliant Green Agar further inhibits Escherichia coli and Proteus spp. Osborne and Stokes used 0.1% Sodium Sulfapyridine to enhance the recovery of Salmonella from whole egg and egg yolk.For more information visit https://www.neogen.com/solutions/Microbiology/brilliant-green-agar-with-sulfapyridine
NEOGEN Dextrose Tryptone Agar, 5 kg
Dextrose Tryptone Agar is used for isolation of mesophilic or thermophilic spoilage microorganisms from food and is not intended for use in the diagnosis of disease or other conditions in humans.Dextrose Tryptone Agar evolved from research by Williams, while studying the cultivation and enumeration of thermophilic bacteria caused by “flat-sour” spoilage of canned foods. In the 1930’s, the National Canners Association specified the use of Dextrose Tryptone Agar for isolating “flat sour” organisms from food products. “Flat sour” spoilage of canned foods is caused by Bacillus coagulans (Bacillus thermoacidurans). Bacterial growth results in a 0.3 – 0.5 drop in pH, while ends of the can remain flat. B. coagulans is a soil microorganism, found in canned tomato products and dairy products. Conditions favorable for organism growth can result in spoilage of food products.For more information visit https://www.neogen.com/solutions/Microbiology/dextrose-tryptone-agar
NEOGEN Clostridium Difficile Agar Base, 500 g
Clostridium Difficile Agar Base (Brazier’s) is used with blood, Cycloserine, and Cefoxitin for the isolation of Clostridium difficile. Clostridium Difficile is not intended for use in the diagnosis of disease or other conditions in humans.Brazier’s CCEY agar is the formulation currently used by the Anaerobe Reference Unit for the isolation of C. difficile, resulting from work initiated by Ken Phillips and Paul Levett, and completed by Jon Brazier. Based upon the market leading anaerobe medium, Fastidious Anaerobe Agar, it incorporates additional ingredients to improve the isolation and differentiation of C. difficile from samples.Cholic acid is present to promote spore germination following alcohol shock treatment, and p-hydroxyphenylacetic acid to enhance the production of p-cresol, a distinctive metabolite of C. difficile.For more information visit https://www.neogen.com/solutions/Microbiology/clostridium-difficile-agar-base
NEOGEN Letheen Agar Base Modified without Tween, 500 g
Letheen Agar Base Modified (without Tween) is used with Tween 80 (Polysorbate 80) for the isolation of microorganisms from cosmetics in a laboratory setting. Letheen Agar Base Modified is not intended for use in the diagnosis of disease or other conditions in humans.In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Tween 80 (Polysorbate 80) to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds in testing of germicidal activity. Total neutralization of disinfectants is critical. Disinfectant residues can result in a false negative (no-growth) test.For more information visit https://www.neogen.com/solutions/Microbiology/letheen-agar-base-modified-without-tween
NEOGEN OGYE Agar Base 500g
OGYE Agar Base is used with oxytetracycline in the detection and isolation of yeasts and molds from foods in a laboratory setting. OGYE Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. Mossel, Visser, and Mengerink described Oxytetracycline-Glucose Yeast Extract (OGYE) Agar for the detection and enumeration of yeasts and molds in foods and other specimens of sanitary significance. Mossel et al. described improved recovery of yeasts and molds compared to acidified agar. This medium is supplemented with oxytetracycline as a selective agent, rather than relying on the low pH of acidified agar to suppress bacterial growth. Acidified agar is commonly used for detection of yeasts and molds in foods and dairy products, and OGYE Agar Base provides an alternate medium. For more information visit https://www.neogen.com/solutions/Microbiology/ogye-agar-base
NEOGEN Muller-Kauffmann Tetrathionate-Novobiocin (MKTTn) Broth, 5 kg
Muller-Kauffmann Tetrathionate-Novobiocin Broth is used for the selective and differential isolation of Salmonella spp. in a laboratory setting and is included in ISO 6579:2017. This medium is not intended for use in the diagnosis of disease or other conditions in humans. A selective enrichment medium for the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. Mueller originally described a tetrathionate broth that inhibited lactose fermenting Enterobacteriaceae but not Salmonella. Kauffmann later modified the formulation to include ox bile and brilliant green to improve selectivity. Finally, Jeffries added Novobiocin to inhibit Proteus species. Meat extract and casein provide a source of nitrogen and amino acids and sodium chloride maintain the osmotic balance.For more information visit https://www.neogen.com/solutions/Microbiology/muller-kauffmann-tetrathionate-novobiocin-broth
NEOGEN Dextrose Tryptone Agar, 500 g
Dextrose Tryptone Agar is used for isolation of mesophilic or thermophilic spoilage microorganisms from food and is not intended for use in the diagnosis of disease or other conditions in humans.Dextrose Tryptone Agar evolved from research by Williams, while studying the cultivation and enumeration of thermophilic bacteria caused by “flat-sour” spoilage of canned foods. In the 1930’s, the National Canners Association specified the use of Dextrose Tryptone Agar for isolating “flat sour” organisms from food products. “Flat sour” spoilage of canned foods is caused by Bacillus coagulans (Bacillus thermoacidurans). Bacterial growth results in a 0.3 – 0.5 drop in pH, while ends of the can remain flat. B. coagulans is a soil microorganism, found in canned tomato products and dairy products. Conditions favorable for organism growth can result in spoilage of food products.For more information visit https://www.neogen.com/solutions/Microbiology/dextrose-tryptone-agar
NEOGEN Lysine Iron Agar, 500 g
Lysine Iron Agar is used for the differentiation of microorganisms on the basis of lysine decarboxylase and hydrogen sulfide production. Lysine Iron Agar is not intended for use in the diagnosis of disease or other conditions in humans. Lysine Iron Agar is prepared according to the formulation of Edwards and Fife, who developed the medium to detect Salmonella arizonae. S. arizonae ferments lactose rapidly, and the authors found expected H2S production on Triple Sugar Iron Agar was suppressed. Detection of S. arizonae is important because it has been implicated in food borne infections. By eliminating lactose and incorporating lysine, Edwards and Fife devised a medium differentiating enteric bacilli based on their ability to decarboxylate or deaminate lysine and produce abundant hydrogen sulfide. This medium is recommended for detecting rapid lactose-fermenting S. arizonae. For more information visit https://www.neogen.com/solutions/Microbiology/lysine-iron-agar
NEOGEN Tryptose, 500 g
Tryptose is an enzymatic digest of protein for use in preparing microbiological culture media in a laboratory setting. Tryptose is not intended for use in the diagnosis of disease or other conditions in humans. Tryptose was developed while studying growth requirements of Brucella spp. Huddleson found Tryptose media to be equal or superior to meat infusion media, providing uniformity for the cultivation and differentiation of fastidious organisms including Neisseria gonorrhoeae, Streptococcus milleri and Brucella spp.. Casman reported that a medium consisting of 2% Tryptose, along with other media ingredients, equaled fresh beef infusion base with respect to organism growth. Tryptose is recommended in broth and agar formulations, and when used in media containing blood to permit hemolytic reactions. Tryptose is used in the preparation of Tryptose Broth (Product Code No. NCM0087).For more information visit https://www.neogen.com/solutions/Microbiology/tryptose
NEOGEN Pseudomonas Isolation Agar, 500 g
Pseudomonas Isolation Agar is used for the isolation of Pseudomonas aeruginosa and other Pseudomonas spp. in a laboratory setting. Pseudomonas Isolation Agar is not intended for use in the diagnosis of disease or other conditions in humans. Pseudomonas Isolation Agar is based on Medium A described by King, Ward, and Raney. This medium is very useful for isolating Pseudomonas spp. Pseudomonas Isolation Agar includes Irgasan®, a potent broad spectrum antimicrobial not active against Pseudomonas spp. This medium is selective and formulated to enhanced formation of blue or blue-green pyocyanin pigment by Pseudomonas aeruginosa. The pigment diffuses into the medium surrounding growth.For more information visit https://www.neogen.com/solutions/Microbiology/pseudomonas-isolation-agar
NEOGEN Casman Medium Base, 10 kg
Casman Medium Base is used with blood for the isolation of Haemophilus influenzae, Neisseria gonorrhoeae and other fastidious microorganisms in a laboratory setting. Casman Medium Base is not intended for use in the diagnosis of disease or other conditions in humans.In 1947, Casman described an infusion-free medium enriched with 5% blood for fastidious microorganisms incubated anaerobically. This medium replaced formulas containing fresh meat infusion and heated blood. Casman adjusted the formula after experiments revealed nicotinamide disrupted the action of a blood enzyme that inactivates V factor (NAD). The concentration of nicotinamide was lowered to support growth of Neisseria spp.For more information visit https://www.neogen.com/solutions/Microbiology/casman-medium-base
NEOGEN Schaedler Agar, 500 g
Schaedler Agar is used for the cultivation of anaerobic microorganisms in a laboratory setting. Schaedler Agar is not intended for use in the diagnosis of disease or other conditions in humans. Survival of anaerobic bacteria is dependent on their sensitivity to oxygen, nutritional requirements, appropriate collection, culture medium, and incubation time and temperature. Schaedler Agar is suitable for standard procedures used in cultivating anaerobic bacteria. Schaedler Agar is prepared according to the formulation described by Schaedler, Dubos, and Costello, and modified by Mata, Carrillo, and Villatoro. Modifications include reduced dextrose to avoid interference with hemolytic reactions, reduced yeast extract to avoid darkening of the medium, and adjusted sodium chloride and nitrogen concentrations.For more information visit https://www.neogen.com/solutions/Microbiology/schaedler-agar
NEOGEN Letheen Broth Base without Tween, 500 g
Letheen Broth Base (no tween) is used with Tween 80 (Polysorbate 80) for the testing of samples containing quaternary ammonium compounds for antimicrobial activity in a laboratory setting. Letheen Broth Base (no tween) is not intended for use in the diagnosis of disease or other conditions in humans.In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Polysorbate 80 to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds while testing germicidal activity. Letheen Agar is a modification of TGE Agar with the addition of Lecithin and Polysorbate 80.Letheen Broth Base was developed as a subculture medium for the neutralization of quaternary ammonium compounds in disinfectant testing.For more information visit https://www.neogen.com/solutions/Microbiology/letheen-broth-base-without-tween
NEOGEN Rappaport-Vassiliadis Medium with Soya (RVS) (ISO), 5 kg
Rappaport-Vassiliadis Medium with Soya is used as a selective enrichment medium for the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. RVS is not intended for use in the diagnosis of disease or other conditions in humans.Rappaport et al. originally described the broth, which was later modified by Vassiliadis et al. and optimized by van Schothorst et al. Enzymatic digest of soya provides a source of nitrogen and amino acids and sodium chloride provides osmotic pressure. The potassium phosphate components act as a buffer. Magnesium chloride and malachite green act as selective agents against non-target microorganisms. The formulation is very hygroscopic and will produce a slight exothermic reaction when mixed with water.For more information visit https://www.neogen.com/solutions/Microbiology/rappaport-vassiliadis-medium-with-soya