Additional Selective and Differential Cell Culture Media
NEOGEN Harlequin E. coli/Coliform Agar, 500 g
E. coli / Coliform Agar is for the simultaneous enumeration of Escherichia coli and coliforms in food and environmental samples and is not intended for use in the diagnosis of disease or other conditions in humans.This dual chromogenic substrate medium makes the different colony types easy to distinguish allowing rapid counting of both E. coli and coliforms on a single agar.Based upon the formulation of Tryptone Bile Agar, the medium has been modified by the addition of two chromogenic substrates, one to detect the ß-glucuronidase enzyme (X- glucuronide) and another to detect the ß-galactosidase enzyme (magenta-ß-gal). Typical E. coli strains possess both enzymes but only cleave the X-glucuronide substrate, thereby producing blue-green colonies. Typical coliforms, however, possess only the ß-galactosidase enzyme and produce rose-pink colonies.For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-e-coli-coliform-agar
NEOGEN Terrific Broth, Modified, 10 kg
Terrific Broth, Modified is used with glycerol in cultivating recombinant strains of Escherichia coli in a laboratory setting. Terrific Broth, Modified is not intended for use in the diagnosis of disease or other conditions in humans. Terrific Broth, Modified was developed by Tartoff and Hobbs to increase yields of plasmid DNA from transformed E. coli and is a popular choice of media available for plasmid preps. While cells grown in Terrific Broth, Modified can grow slower than other liquid media, the final cell density and plasmid yields are 2 – 4 fold higher. The higher yield is a result of the increased concentration of Enzymatic Digest of Casein and Yeast Extract in this formula. Glycerol is used as a carbohydrate source, and unlike glucose, is not fermented to acetic acid.For more information visit https://www.neogen.com/solutions/Microbiology/terrific-broth-modified
NEOGEN Harlequin Sorbitol MacConkey Agar (SMAC), 500 g
Sorbitol MacConkey Agar (SMAC) is for the isolation of Escherichia coli O157:H7 and is not intended for use in the diagnosis of disease or other conditions in humans. Pathogenicity of Escherichia coli O157:H7, the primary serovar associated with haemorrhagic colitis (HC) and haemolytic uraemic syndrome (HUS), is linked to the production of verocytotoxins (VT1 and VT2). However, it should be noted that not all strains of O157 produce verocytotoxins, and that strains from other serovars can be toxin producers (e.g. O26, O103, O111, O113, O145). E. coli O157 has been associated epidemiologically with food poisoning outbreaks involving beef burgers and cold cooked meats. This medium is a modification of Sorbitol MacConkey Agar (SMAC).For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-sorbitol-macconkey-agar
NEOGEN Bacteriological Peptone, 500 g
Bacteriological Peptone is for use in preparing microbiological culture media in a laboratory setting, and is not intended for use in the diagnosis of disease or other conditions in humans.An economical source of nutrients provided by a balanced mixture of meat peptones and tryptone. The growth requirements of most non-fastidious organisms will be fulfilled by the range of amino acids, peptides and proteoses in this mixture.For more information visit https://www.neogen.com/solutions/Microbiology/bacteriological-peptone
NEOGEN Acuferm Peptone, 500 g
For more information visit https://www.neogen.com/solutions/Microbiology/acuferm-peptone
NEOGEN Fluid Thioglycolate Medium, 10Kg
Fluid Thioglycolate Medium is used for sterility testing. This medium conforms to Harmonized USP/EP/JP requirements and is not intended for use in the diagnosis of disease or other conditions in humans.Fluid Thioglycolate Medium is recommended by the Harmonized Pharmacopoeia for sterility testing and conforms to USP/EP/JP performance specification. In this formulation casein and yeast extract provide a source of nitrogen, essential vitamins and amino acids. The glucose provides a carbon source and sodium chloride maintains osmotic balance. L-Cystine and sodium Thioglycolate act as reducing agents to create an anaerobic environment and maintain a low Eh. This is aided by the low level of agar which reduces the oxygen permeability through the medium. Resazurin is an oxidation indicator which turns from colorless to red/pink when oxidised.For more information visit https://www.neogen.com/solutions/fluid-thioglycollate-medium-ncm0108
NEOGEN Sodium Chloride (Bacteriological), 500 g
Sodium Chloride is a salt often used when preparing microbiological culture media in a laboratory setting. Sodium Chloride is not intended for use in the diagnosis of disease or other conditions in humans. When added to culture media Sodium Chloride allows for adjustments in osmotic pressure.For more information visit https://www.neogen.com/solutions/Microbiology/sodium-chloride-bacteriological
NEOGEN Tryptose No. 2, 500 g
Tryptose No. 2 is a special blend of peptones developed for use in the production of Foot and Mouth vaccine, and is not intended for use in the diagnosis of disease or other conditions in humans.A special blend of peptones originally developed for use in the production of Foot and Mouth vaccine. Other peptone blends are also available within the catalogue, some of which have been evaluated to demonstrate low cell toxicity and the ability to enhance the growth of cell lines (eg NCM0264).For more information visit https://www.neogen.com/solutions/Microbiology/tryptose-number-2
NEOGEN Vogel Johnson Agar, 500 g
Vogel Johnson Agar is used for the isolation of staphylococci in a laboratory setting. Vogel Johnson Agar is not intended for use in the diagnosis of disease or other conditions in humans. Coagulase-positive staphylococci, primarily Staphylococcus aureus, are among the microorganisms that cause spoilage or chemical changes in cosmetic products. To isolate coagulase-positive, mannitol fermenting staphylococci, Vogel and Johnson modified Tellurite-Glycine Agar by Zebovitz, Evans, and Niven. The modification included increasing mannitol and adding a pH indicator. Vogel Johnson Agar selects and differentiates coagulase-positive staphylococci that ferment mannitol and reduce tellurite. Vogel Johnson (VJ) Agar is specified in standard methods testing for cosmetics and nutritional supplements.For more information visit https://www.neogen.com/solutions/Microbiology/vogel-johnson-agar
NEOGEN Fluid Thioglycolate Medium, 500g
Fluid Thioglycolate Medium is used for sterility testing. This medium conforms to Harmonized USP/EP/JP requirements and is not intended for use in the diagnosis of disease or other conditions in humans.Fluid Thioglycolate Medium is recommended by the Harmonized Pharmacopoeia for sterility testing and conforms to USP/EP/JP performance specification. In this formulation casein and yeast extract provide a source of nitrogen, essential vitamins and amino acids. The glucose provides a carbon source and sodium chloride maintains osmotic balance. L-Cystine and sodium Thioglycolate act as reducing agents to create an anaerobic environment and maintain a low Eh. This is aided by the low level of agar which reduces the oxygen permeability through the medium. Resazurin is an oxidation indicator which turns from colorless to red/pink when oxidised.For more information visit https://www.neogen.com/solutions/fluid-thioglycollate-medium-ncm0108
NEOGEN Bacillus cereus MYP Agar, 5 kg
Bacillus Cereus MYP Agar is used with supplements for the selective and differential enumeration of Bacillus cereus from foods. Conforms to ISO and FDA / BAM formulation in a laboratory setting. MYP Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.Formulated to ISO 7932:2004 MYP was introduced by Mossel and his co-workers in 1967 for the enumeration of Bacillus cereus in foods and was shown to be the most effective for this purpose by Inal in 1972. B. cereus is differentiated from other members of the Bacillus group by two reactions: mannitol fermentation and lecithinase production. Mannitol fermentation on this medium produces a yellow color. B. cereus is mannitol negative and produces red colonies. The lecithinase production of B. cereus is indicated by a white precipitate around the colonies.For more information visit https://www.neogen.com/solutions/Microbiology/bacillus-cereus-myp-agar
NEOGEN Listeria Enrichment Broth, 500 g
Listeria Enrichment Broth is used for selective enrichment of Listeria spp and is not intended for use in the diagnosis of disease or other conditions in humans.Listeria monocytogenes, first described in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage, and river water.For more information visit https://www.neogen.com/solutions/Microbiology/listeria-enrichment-broth
NEOGEN Tryptic Soy Broth, Modified with Novobiocin, 10 kg
Tryptic Soy Broth, Modified with Novobiocin is used for the selective enrichment of enterohemorrhagic E. coli in foods in a laboratory setting. Tryptic Soy Broth, Modified with Novobiocin is not intended for use in the diagnosis of disease or other conditions in humans. The first major outbreak of E. coli O157:H7 was in 1982 and traced to contaminated hamburgers. Other known sources of infection include sprouts, lettuce, salami, unpasteurized milk, juice and/or swimming in or drinking contaminated water. Tryptic Soy Broth, Modified with Novobiocin is used to enrich food samples suspected of having low levels of EHEC during pathogen testing.For more information visit https://www.neogen.com/solutions/Microbiology/tryptic-soy-broth-modified-with-novobiocin
NEOGEN Perfringens Agar Base (TSC), 10 kg
Perfringens Agar Base (TSC) is used with selective and differential supplements for the recovery of Clostridium perfringens, and is not intended for use in the diagnosis of disease or other conditions in humans. Formulated according to ISO 14189:2013, Perfringens Agar Base (TSC) is a nutritious base medium used in the preparation of SFP Agar and TSC Agar. Depending upon the formula, supplements are added to increase the selectivity of the medium. Shahidi-Ferguson Perfringens (SFP) Agar is based on the formula by Shahidi and Ferguson and incorporates the supplements, Polymyxin B Sulfate and Kanamycin. Tryptose Sulphite Cycloserine (TSC) Agar is developed with the same base as SFP Agar, with the addition of Cycloserine. If desired, Egg Yolk Emulsion can be added to either formula.For more information visit https://www.neogen.com/solutions/Microbiology/perfringens-agar-base
NEOGEN Orange Serum Agar, 10 kg
Orange Serum Agar is used for the cultivation of aciduric microorganisms associated with spoilage of products and is not intended for use in the diagnosis of disease or other conditions in humans.The low pH of fruit juices makes citrus fruit products susceptible to spoilage by yeasts, molds, and the bacteria Lactobacillus and Leuconostoc. In the 1950’s, Hays investigated spoilage in frozen concentrated orange juice. He found that an agar medium containing orange serum (juice) was superior to Lindegren Agar in isolating the microorganisms responsible for spoilage causing a buttermilk off-odor. Murdock, Folinazzo, and Troy found Orange Serum Agar, pH 5.4 to be a suitable medium for growing Leuconostoc, Lactobacillus, and yeasts. Orange Serum Agar is recommended for examining fruit beverages.For more information visit https://www.neogen.com/solutions/Microbiology/orange-serum-agar