Additional Selective and Differential Cell Culture Media
NEOGEN Nutrient Agar (BAM), 500 g
Nutrient Agar (BAM) is used for the cultivation of a wide variety of microorganisms in a laboratory setting. Nutrient Agar is not intended for use in the diagnosis of disease or other conditions in humans. In the early 1900’s, the American Public Health Association (APHA) suggested the formula of Nutrient Agar as a standard culture medium used in water testing. Nutrient Agar continues to be a widely used general purpose medium for growing non-fastidious microorganisms. If required, enrichments can be added to this medium. Nutrient Agar, modified by incorporating 4-methylumbelliferyl-β-D-glucuronide (MUG), is used for fluorogenic detection of Escherichia coli. Nutrient Agar meets APHA and Association of Official Analytical Chemists (AOAC) standard methods. Nutrient Agar is specified in many standard methods procedures for the examination of food, dairy products, water, and other materials.For more information visit https://www.neogen.com/solutions/Microbiology/nutrient-agar-bam
STEMCELL Technologies STEMdiff™ Endothelial Differentiation Kit, Size: 1 Kit
STEMdiff™ Endothelial Differentiation Kit (Catalog #08005) includes attachment substrate, animal component-free (ACF) endothelial induction medium, and endothelial expansion medium. It is optimized for the differentiation of human pluripotent stem cells (hPSCs) maintained in mTeSR™1 (Catalog #85850), mTeSR™ Plus (Catalog #05825), or TeSR™-E8™ (Catalog #05990) on Corning Matrigel to endothelial-like cells. The kit is designed to be used immediately after early mesoderm induction with STEMdiff™ Mesoderm Induction Medium (Catalog #05220), available for purchase separately.CollPlant (https://collplant.com/) is the manufacturer of the rhCollagen component of the cell attachment substrate.
Promocell GmbH PromoCell Endothelial Cell Growth Medium MV 500 ml, ready-to-use, low serum, with C-39225 attached to the flask
Endothelial Cell Growth Medium MV is a low-serum (5% V/V) medium developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, murine, rat and dog endothelial cells from microvascular vessels as well as for various endothelial cell lines.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.05 ml / ml || Endothelial Cell Growth Supplement | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Heparin | 90 µg / ml || Hydrocortisone | 1 µg / ml |For best transfection results we recommend using our heparin-free Endothelial Cell Growth Medium MV2. Heparin, included in all our other Endothelial Cell Growth Media formulations, may reduce transfection efficiency.
Promocell GmbH PromoCell Supplement Pack / Keratinocyte Growth Medium 2 Contains: C-30020, C-30221, C-31010, C-31059, C-31400, C-31110, C-34005
PromoCell Keratinocyte Growth Medium 2 is a serum-free medium developed for the cultivation of epidermal keratinocytes without feeder cells.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for horse and porcine keratinocytes, as well as mouse and rat keratinocytes after adapting the CaCl2 concentration. In addition, it is suited for rat corneal keratinocytes.| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 0.125 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.33 μg / ml || Epinephrine | 0.39 μg / ml || Transferrin (recombinant human) | 10 μg / ml || CaCl2 | 0.06 mM |
Promocell GmbH PromoCell Fibroblast Basal Medium 2 (prf) 500 ml, phenol red-free
PromoCell Fibroblast Growth Medium 2 is a low-serum (2% V/V) medium and has been developed for the in vitro cultivation of adult Human Fibroblasts.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, and rat fibroblasts, as well as fibroblast cell lines.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.02 ml / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 5 μg / ml |
NEXPRING HEALTH Modified HTF Medium with Gentamicin - HEPES, 500 mL
Modified HTF Medium is a synthetic, defined solution for procedures which include the retrieval, handling and transfer of human gametes and embryos. mHTF is composed of a HEPES and sodium bicarbonate buffering system, making it ideal for use in ambient atmosphere conditions. It requires protein supplementation before use.
United States Biological Corporation Dulbecco’s MEM Powder, 10 L
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.
Promocell GmbH PromoCell DC Base Medium 250 ml, with C-39853 attached to flask
The PromoCell Dendritic Cell (DC) Generation Medium has been developed for the easy and efficient generation of immature as well as fully mature myeloid Dendritic Cells from peripheral blood monocytes. The DC Base Medium is a user-customizable versions of the DC Generation Media that does not include cytokines.PromoCell DC Generation Medium allows for the generation of DC from cryopreserved as well as freshly isolated cells. In the latter case, the Monocyte Attachment Medium is also needed.PromoCell DC Media consist of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete Medium. Indeed, all DC Media must be supplemented with additional cytokines. The cytokines are included with the DC Generation Media but not with the DC Base Media.
Lonza Walkersville Human Cryopreserved Hepatocyte Thawing Medium
Human Cryopreserved Hepatocyte Thawing Medium is supplied ready to use in 50mL conical tubes. Medium has been optimized specifically for single-donor human primary hepatocytes to ensure high viability and yield is maintained during reanimation from cryopreservation.Benefits:Optimized for maintaining hepatocyte cell health during thawingApplications:ADMEToxContent:50mL conical tube
Lonza Walkersville MM250 Hepatocyte Maintenance Medium
Serum-free hepatocyte maintenance medium for optimal performance of primary hepatocytes in monolayer or sandwich culture formats.This maintenance medium is specifically required for mouse plateable hepatocyte products.Benefits:Optimized for Hepatocyte Cell healthApplications:ADMEToxContent:250mLs basal medium 2.5mL supplement
Lifeline Cell Technology M MICROV HU LIFELINE KT
Includes VascuLife Basal Medium 475 mL, VascuLife VEGF-Mv LifeFactors (with Vascular Endothelial Growth Factor). Optimized for the culture of human microvascular endothelial cells in a 5% serum environment. No antimicrobials, no phenol red