Additional Selective and Differential Cell Culture Media
NEOGEN Lauryl Tryptose (LST) Broth, 500 g
Lauryl Tryptose (LST) Broth is used for the detection of coliform bacteria in water and wastewater. Lauryl Tryptose Broth is not intended for use in the diagnosis of disease or other conditions in humans.Lauryl Tryptose Broth is a selective medium for the detection of coliforms in water, dairy products and other foods. The American Public Health Authority (APHA) recommend Lauryl Tryptose Broth for the Most Probable Number Presumptive Test of coliforms in waters, effluent or sewage and as a confirmation test of lactose fermentation with gas production from milk samples and for the detection of coliforms in foods.Lauryl Tryptose Broth is prepared according to the formulation of Mallmann and Darby. Mallmann and Darby showed that tryptose at a concentration of 2% increased the early logarithmic growth phase when compared to meat peptone.For more information visit https://www.neogen.com/solutions/Microbiology/lauryl-tryptose-broth
NEOGEN MacConkey Broth, 500 g
MacConkey Broth is used for the detection of coliform bacteria in milk and water. Conforms to Harmonized USP/EP/JP Requirements and is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for the selective enrichment of Escherichia coli from non-sterile samples. Conforms to Harmonized USP/EP/JP performance specification. Gelatin peptone provides a source of nitrogen, while lactose is a fermentable carbohydrate. Ox bile acts a selective agent inhibiting most Gram-positive organisms and bromocresol purple acts as a pH indicator. A color change from purple to yellow indicates growth of a bile tolerant, lactose fermenting organism such as Escherichia coli. According to the Harmonized USP/EP/JP, MacConkey Broth is used as a selective enrichment broth, with subculture performed onto MacConkey Agar. For more information visit https://www.neogen.com/solutions/Microbiology/macconkey-broth
NEOGEN Blood Agar Base, 10 kg
Blood Agar Base is used with blood for the isolation and cultivation of a wide variety of fastidious microorganisms. Blood Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. An inexpensive, general purpose agar base which, with the addition of 5-7% sterile blood, can be used to cultivate a wide range of microorganisms of indicative significance. Typical hemolysis patterns are obtained with this medium. Formula:Beef Extract - 10.0 g/LBalanced Peptone - 10.0 g/LSodium Chloride - 5.0 g/LAgar - 12.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/blood-agar-base
NEOGEN Columbia Blood Agar Base, 10 kg
Columbia Blood Agar Base is used with blood for the isolation and cultivation of a wide variety of fastidious microorganisms. Columbia Blood Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. A modification of the original Columbia Agar base formulation, Columbia Blood Agar Base provides a medium that is suitable for use with both defibrinated horse or sheep blood. The peptone mixture provides a source of nitrogen, essential vitamins and amino acids. The starch provides a carbon source and sodium chloride maintains osmotic balance. Originally described as a general purpose nutritious agar base by Ellner et al. Columbia Agar is more frequently used when enriched by the addition of sterile blood. The medium is suitable for supporting the growth and determining hemolytic reactions of a variety of microorganisms. For more information visit https://www.neogen.com/solutions/Microbiology/columbia-blood-agar-base
NEOGEN BCYE Agar (Legionella Isolation Medium), 500 g
BCYE Agar is used (with appropriate supplementation) for the isolation of Legionella spp. from water and environmental samples. BCYE Agar is not intended for use in the diagnosis of disease or other conditions in humans.In 1977, McDade et al. identified Legionella pneumophila as the causative agent of Legionnaires’ disease, a multisystem disease manifested primarily by pneumonia. In 1978 a new medium, F-G Agar, resulted in improved growth of L. pneumophila, a very fastidious organism. Freely et al. modified F-C Agar by substituting yeast extract as a vitamin source and replacing starch with activated charcoal, producing Charcoal Yeast Extract (CYE) Agar. In 1980, Pasculle et al. reported that CYE Agar could be improved by the addition of ACES (N-2-acetamido-2-aminoethane sulfonic acid) buffer.For more information visit https://www.neogen.com/solutions/Microbiology/bcye-agar-legionella-isolation-medium
NEOGEN PALCAM Broth, 500 g
PALCAM Broth is used with supplements for the selective enrichment of Listeria spp. from foods and environmental samples in a laboratory setting. PALCAM Broth is not intended for use in the diagnosis of disease or other conditions in humans. Listeria monocytogenes, described in 1926 by Murray, Webb, and Swann, is a widespread problem in food industries. Evidence from outbreaks of listeriosis indicate the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. PALCAM Broth is based on the formulation of Van Netten et al. while he was investigating the isolation of Listeria spp. from food samples. PALCAM Broth has been used for testing Listeria from raw milk.For more information visit https://www.neogen.com/solutions/Microbiology/palcam-broth
NEOGEN Oxford Listeria Agar, 500 g
Oxford Listeria Agar Base is used with antimicrobics for the selective isolation of Listeria spp. in a laboratory setting. Oxford Listeria Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in food industries. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission included turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage and river water.For more information visit https://www.neogen.com/solutions/Microbiology/oxford-listeria-agar
NEOGEN Lauryl Sulfate Broth with MUG, 5 kg
Lauryl Sulfate Broth with MUG is used for the detection of coliforms and the fluorogenic detection of Escherichia coli in a laboratory setting. Lauryl Sulfate Broth with MUG is not intended for use in the diagnosis of disease or other conditions in humans.The coliform group of bacteria includes aerobic and facultative anaerobic, Gram-negative, non-sporeforming bacilli that ferment lactose and form acid and gas at 35°C within 48 hours. Members of the Enterobacteriacae comprise the majority of this group, but organisms such as Aeromonas spp. may also be included. Procedures to detect and confirm coliforms are used in testing water, foods, dairy products, and other materials.Lauryl Sulfate Broth, also referred to as Lauryl Tryptose Broth, is prepared according to the formula of Mallmann and Darby.For more information visit https://www.neogen.com/solutions/Microbiology/lauryl-sulfate-broth-with-mug
NEOGEN Reinforced Clostridial Medium, 500 g
Reinforced Clostridial Medium is used for the cultivation of anaerobes from food. This medium conforms to Harmonized USP/EP/JP Requirements and is not intended for use in the diagnosis of disease or other conditions in humans.Reinforced Clostridial Medium (RCM) is recommended by the Harmonized Pharmacopoeia for the selective enrichment of Clostridia from non-sterile samples and conforms to USP/EP/JP performance specifications. Peptone, beef and yeast extract provide a source of nitrogen, essential vitamins and amino acids. Starch aids the detoxification of harmful metabolites and glucose is a fermentable carbohydrate. Sodium chloride provides osmotic balance and sodium acetate acts as a buffer. L-Cysteine hydrochloride acts as a reducing agent to create an anaerobic environment and maintain a low Eh.For more information visit https://www.neogen.com/solutions/Microbiology/reinforced-clostridial-medium
NEOGEN Universal Pre-enrichment Broth, 500 g
Universal Pre-enrichment Broth is used for the recovery of Salmonella spp. and Listeria spp. in a laboratory setting. Universal Pre-enrichment Broth is not intended for use in the diagnosis of disease or other conditions in humans. Traditional methods for recovering Salmonella spp. and Listeria spp. from food products require separate pre-enrichment media for each microorganism. Some broth media recommended for preenrichment contain antibiotic inhibitors or have insufficient buffering capacity which hinder recovery of sublethally injured cells. Bailey and Cox formulated Universal Preenrichment Broth to permit simultaneous resuscitation of sublethally injured Salmonella and Listeria. The broth medium provides sufficient buffering capacity to prevent rapid decreases in pH and allows for repair of injured cells that might be sensitive to low pH values or inhibitory substances.For more information visit https://www.neogen.com/solutions/Microbiology/universal-preenrichment-broth
NEOGEN Fraser Broth, 5 kg
Fraser Broth is used with ferric ammonium citrate for the selective enrichment of Listeria species and is not intended for use in the diagnosis of disease or other conditions in humans.Fraser Broth is a secondary enrichment broth for the isolation of Listeria spp. formulated according to ISO 11290. The selective components acriflavine and nalidixic acid are blended into the base powder and the ferric ammonium citrate (X211 or 7984) is added to the tempered broth after sterilization.Formula:Peptone - 15.0 g/LYeast Extract - 5.0 g/LEsculin - 1.0 g/LDisodium Hydrogen Phosphate - 9.6 g/LPotassium Dihydrogen Phosphate - 1.35 g/LSodium Chloride - 20.0 g/LLithium Chloride - 3.0 g/LAcriflavine - 0.025 g/LNalidixic Acid - 0.02 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/fraser-broth
NEOGEN EC Broth, 10 kg
EC Broth is used for the detection of coliform bacteria at 37°C and Escherichia coli at 44.5°C. EC Broth is not intended for use in the diagnosis of disease or other conditions in humans.EC Broth (Escherichia coli Broth) is a selective enrichment broth designed for the isolation of coliforms, including E. coli, from water and food samples. It was the recommended medium of the American Public Health Association (APHA) and the AOAC.EC Broth is made selective for coliforms by the inclusion of Bile Salts in the dehydrated medium. The selective nature of this medium ensures that the growth of non-coliform bacteria is minimized. The medium is buffered by the addition of potassium phosphates and osmotically balanced by sodium chloride. The medium is used at 37°C for coliform organisms and 44.5°C is recommended for the isolation E. coli.For more information visit https://www.neogen.com/solutions/Microbiology/ec-broth
NEOGEN Mannitol Salt Agar, 5 kg
Mannitol Salt Agar is used for the isolation of staphylococci in a laboratory setting. Mannitol Salt Agar is not intended for use in the diagnosis of disease or other conditions in humans. Conforms to Harmonized USP/EP/JP Requirements.Chapman formulated Mannitol Salt Agar to isolate staphylococci by inhibiting growth of most other bacteria with a high salt concentration. Chapman added 7.5% Sodium Chloride to Phenol Red Mannitol Agar and noted pathogenic strains of staphylococci (coagulase-positive staphylococci) grew luxuriantly and produced yellow colonies with yellow zones. Non-pathogenic staphylococci produced small red colonies with no color change to the surrounding medium. Mannitol Salt Agar is recommended for isolating pathogenic staphylococci from samples, cosmetics, and microbial limit tests.For more information visit https://www.neogen.com/solutions/Microbiology/mannitol-salt-agar
NEOGEN Peptone Water, 500 g
Peptone Water is used for the cultivation of non-fastidious microorganisms, indole testing, and as a basal medium for carbohydrate fermentation studies. Peptone Water is not intended for use in the diagnosis of disease or other conditions in humans. A general purpose growth medium that can be used as a base for carbohydrate fermentation studies. The medium has a high level of tryptone making it suitable for use in the indole test.For more information visit https://www.neogen.com/solutions/Microbiology/peptone-water
NEOGEN Tryptic Soy Broth, Modified with 8 mg Novobiocin & Acid Digest Casein, 10 kg
Tryptic Soy Broth, Modified with Novobiocin & Acid Digest of Casein is used for the selective enrichment of enterohemorrhagic E. coli in foods. Conforms to USDA Formulation with Novobiocin supplementation in a laboratory setting. Tryptic Soy Broth, Modified with Novobiocin & Acid Digest of Casein is not intended for use in the diagnosis of disease or other conditions in humans. The first major outbreak of E. coli O157:H7 was in 1982, and traced to contaminated hamburgers. Other known sources of infection include sprouts, lettuce, salami, unpasteurized milk, juice and/or swimming in or drinking contaminated water. Tryptic Soy Broth, Modified with Novobiocin & Acid Digest of Casein is used to enrich food samples suspected of having low levels of EHEC during pathogen testing.For more information visit https://www.neogen.com/solutions/Microbiology/tryptic-soy-broth-modified-with-8-mg-novobiocin-and-acid-digest-casein