Additional Selective and Differential Cell Culture Media
Research Products International Corp Trypto Soy Broth, 500 Grams
Dissolve 30 grams in 1 liter of distilled water. Sterilize by autoclaving for 15 minutes.
Formula Per Liter:
Casein Digest Peptone: 17.0 g
Papaic Digest of Soybean Meal: 3.0 g
Dipotassium Phosphate: 2.5 g
Dextrose: 2.5 g
Sodium Chloride: 5.0 g
Bioworld Murashige and Skoog(MS) Medium, Modification No. 3, 25 L
Murashige & Skoog (MS) MediumContains Micronutrients and Macronutrients with 1/2 Concentration of NH4NO3 and KNO3 as described by Murashige & Skoog (1967) Sucrose, Cytokines, Vitamins, and Auxins should be added as needed Provided as 1 unit to make 10L, 25L, or 50L of medium, or as 10 units to make 1L of medium Plant tissue culture tested
Creative Biomart Porcine Pepsin
The product is a potent enzyme in gastric juice that digests proteins such as those in meat, eggs, seeds, and dairy products.Studies on gastric digestion from 1820-1840 led to the discovery of the product as the substance which, in the presence of stomach acid, causes nutrients including meat or coagulated egg whites to dissolve. Soon afterward, it was shown that these protein nutrients were cleaved by the product to products called peptones.
Bioworld Leifert and Waites Sterility Test Medium, 500 g
Leifert & Waites Sterility Test MediumContains the Micronutrients, Macronutrients, Vitamins, Sucrose, and Plant Growth Regulators required to initiate plant growth and support the growth of any microorganisms in the explantUsed to determine and identify potential pathogens in explantsCytokines and Auxins should be added as needed Plant tissue culture testedWorking Concentration: 38.2 g/LpH: 6.25–7.25Soluble in water
Quality Biological Inc Cell Freezing Medium A 2X (Antibiotic Freezing Medium), 50ML
Cell Freezing or cryopreservation ensures that you have back-up cells in case of contamination and loss of cell supply. It is best to cryopreserve cells when they are at their maximum growth rate or almost confluent. Cell Freezing Medium A is frequently used in cell biology research for the cryopreservation of cells. Cell Freezing Medium A (2X) works with Cell Freezing Medium B (2X).
NEOGEN PPLO Broth Base without Crystal Violet, 10 kg
PPLO Broth Base without Crystal Violet is used with enrichments for the isolation and cultivation of Mycoplasma spp. in a laboratory setting. PPLO Broth without Crystal Violet is not intended for the use in the diagnosis of disease or other conditions in humans. PPLO Broth Base without Crystal Violet is prepared according to the formula described by Morton and Lecce. Crystal Violet may inhibit the growth of Mycoplasma, and therefore was removed from an earlier formula described by Morton, Smith, Williams, and Eikenberg. Mycoplasma was discovered in a case of pleuropneumonia in a cow, and referred to as “pleuropneumonia-like organism” or PPLO. Mycoplasmas belong to the class of Mollicutes “soft skin”, which are the smallest free-living organisms. They are pleomorphic, varying in size from 0.2 to 0.3 μm.For more information visit https://www.neogen.com/solutions/Microbiology/pplo-broth-base-without-crystal-violet
NEOGEN Triple Sugar Iron (TSI) Agar, 5 kg
Triple Sugar Iron (TSI) Agar is used for the differentiation of microorganisms on the basis of dextrose, lactose, and sucrose fermentation and hydrogen sulfide production. Triple Sugar Iron (TSI) Agar is not intended for use in the diagnosis of disease or other conditions in humans. This is a modification of the Krumwiede and Kohn medium of 1917 which differentiates some of the Enterobacteriaceae on the basis of four reactions; fermentation of lactose, glucose and sucrose and H2S production. This medium should be used in conjunction with a urease test to eliminate Proteus spp. when screening for Salmonella spp.For more information visit https://www.neogen.com/solutions/Microbiology/triple-sugar-iron-tsi-agar
NEOGEN Letheen Broth with Tween, Modified, 10 kg
Letheen Broth with Tween, Modified is used for the recovery of microorganisms from cosmetics in a laboratory setting and is not intended for use in the diagnosis of disease or other conditions in humans.In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Polysorbate 80 to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds in testing of germicidal activity. Total neutralization of disinfectants is critical as disinfectant residues can result in a false negative (no-growth) test result.Letheen Broth with Tween, Modified is based on the formula described in FDA Bacteriological Analytical Manual, and a modification of Letheen Broth Base.For more information visit https://www.neogen.com/solutions/Microbiology/letheen-broth-with-tween-modified
NEOGEN Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar, 5 kg
Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar is used for the selective isolation of Vibrio cholerae and other enteropathogenic vibrios according to ISO 21872-1:2017 and ISO 21872-2:2007. Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar is not intended for use in the diagnosis of disease or other conditions in humans. TCBS Agar is also called Vibrio Selective Agar, and prepared according to the formula of Kobayashi et al. This formula is a modification of the selective medium from Nakanishi. All pathogenic Vibrio spp., except Vibrio hollisae, will grow on TCBS Agar. This highly selective agar meets the nutritional requirements of Vibrio spp. Vibrio spp. are able to grow in media containing increased salt concentrations, and some species are halophilic.For more information visit https://www.neogen.com/solutions/Microbiology/thiosulfate-citrate-bile-salts-sucrose-agar
NEOGEN Brilliant Green Agar (Modified), 5 kg
Brilliant Green Agar is used for the selection and differentiation of Salmonella (other than S. typhi) from foods or feedstuffs. Brilliant Green Agar is not intended for use in the diagnosis of disease or other conditions in humans. First introduced by Kristensen et al in 1925 as a selective medium for the isolation of Salmonella (except S. typhi). The medium was modified by the Netherlands Institute for Public Health, Utrecht. The modification was to increase the selectivity of the medium by increasing the dye concentration. This formulation is quoted by the International Standards Organization, standard European Community Methods, the American Public Health Association and the Association of Official Analytical Chemists. The medium is suitable for subcultures from selective enrichment media. However, because this medium is highly selective, small numbers of salmonellae may be missed.For more information visit https://www.neogen.com/solutions/Microbiology/brilliant-green-agar
NEOGEN Buffered Peptone Water (BPW), 5 kg
Buffered Peptone Water (BPW) is used for the non-selective pre-enrichment of Salmonella spp. from food and is not intended for use in the diagnosis of disease or other conditions in humans. Edel and Kamelmacher found that food preservation techniques involving heat, desiccation, preservatives, high osmotic pressure, or pH changes cause sublethal injury to Salmonella spp. Preenrichment in a non-selective medium allows for repair of cell damage and facilitates the recovery of Salmonella. Lactose Broth is frequently used for this purpose, but it may be detrimental to recovering Salmonellae. Buffered Peptone Water maintains a high pH over the pre-enrichment period and allows in repair of injured cells that may be sensitive to low pH. This is particularly important for vegetable specimens which have a low buffering capacity. Buffered Peptone Water is used in standard methods. For more information visit https://www.neogen.com/solutions/Microbiology/buffered-peptone-water