Additional Selective and Differential Cell Culture Media
ILEX LIFE SCIENCES LLC WILLIAMS E P04-29150 500ML
PAN-Biotech William's Medium E, w: stable Glutamine, w: 2.24 g/L NaHCO3 (500 ml). BICARBONATE: With 2.24 g/L Sodium Bicarbonate. GLUCOSE: With glucose. GLUTAMINE: With Stable Glutamine. PHENOL RED: With Phenol Red. HEPES: Without HEPES. SODIUM PYRUVATE: With sodium pyruvate. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany. DESCRIPTION: Williams and Gunn originally developed William's E Medium as a reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. William's E Medium may also be used for the cultivation of primary hepatocyte cells of other species as well as human-derived HepaRG cells. The medium is unique in that it contains zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione, and the lipid methyl linoleate.
Promocell GmbH PromoCell Endothelial Cell Growth Medium KIT 500 ml, Contains: C-22210, C-39210
Endothelial Cell Growth Medium is a low-serum (2% V/V) medium optimized for the cultivation of endothelial cells from large blood vessels.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, murine, caprine, rabbit, elephant, dog, and rat endothelial cells from large vessels as well as for various endothelial cell lines.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.02 ml / ml || Endothelial Cell Growth Supplement | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 0.1 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Heparin | 90 μg / ml || Hydrocortisone | 1 μg / ml |Note: For best transfection results we recommend using our heparin-free Endothelial Cell Growth Medium MV2.
Promocell GmbH PromoCell Supplement Mix / Endothelial Cell Growth Medium 2
Endothelial Cell Growth Medium 2 is a low-serum (2% V/V) medium that lacks Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains Insulin-like Growth Factor (Long R3 IGF) and Vascular Endothelial Growth Factor (VEGF). Because of its effects on cell metabolism, VEGF may interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.02 ml / ml || Epidermal Growth Factor (recombinant human) | 5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 10 ng / ml || Insulin-like Growth Factor (Long R3 IGF) | 20 ng / ml || Vascular Endothelial Growth Factor 165 (recombinant human) | 0.5 ng / ml || Ascorbic Acid | 1 µg / ml || Heparin | 22.5 μg / ml || Hydrocortisone | 0.2 µg / ml |For best transfection results we recommend using our heparin-free Endothelial Cell Growth Medium MV2.
Promocell GmbH PromoCell Osteoblast Growth Medium 500 ml, ready-to-use, low-serum, with C-39615 attached to the flask
PromoCell Osteoblast Growth Medium has been optimized for the in vitro cultivation of human osteoblasts. The fetal calf serum in the medium is strictly pretested for optimal performance.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, murine, and rat osteoblasts as well as osteoblast cell lines.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* SupplementMix: Contains all media supplements premixed in one vial| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.1 ml / ml |
Promocell GmbH PromoCell Adipocyte Basal Medium 500 ml
PromoCell Adipocyte Nutrition Medium is a low-serum (3% V/V) medium developed for the in vitro cultivation of mature adipocytes.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for murine preadipocytes/adipocytes.Components:* Nutrition Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Nutrition Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.03 ml / ml || d-Biotin | 8 μg / ml || Insulin (recombinant human) | 0.5 μg / ml || Dexamethasone | 400 ng / ml |
INTACT GENOMICS INC IG Autoinduction Medium, 1L
This autoinduction medium is designed to simplify and enhance the production of T7 promoter-regulated recombinant proteins in E. coli. It eliminates the need for manual induction and frequent optical density monitoring, providing a “set-and-forget” approach. This results in repeatable, high-yield protein expression and high cell densities.
- Eliminates frequent OD600 monitoring or manual inducer addition
- Provides reliable and consistent protein expression
- Reduces issues like leaky expression, vector instability, and challenges with toxic proteins
- Promotes high cell density and robust protein expression
- Compatible with other DE3 strains and media
- Automates protein expression after inoculation
Discovery Life Sciences HEPATOCYTE INDUCTION MEDIA 50M
HIM- hepatocyte induction media with supplement 50ml
NEOGEN Tryptic Soy Broth (Soybean-Casein Digest Broth), 10 kg
Tryptic Soy Broth (Soybean-Casein Digest Broth) is used for the cultivation of a wide variety of microorganisms. Tryptic Soy Broth conforms to Harmonized USP/EP/JP requirements and is not intended for use in the diagnosis of disease or other conditions in humans.Tryptic Soy Broth, a general purpose medium, is commonly referred to as Casein Soya Bean or Soybean-Casein Digest broth, and abbreviated as TSB. This medium was originally developed for use without blood in determining the effectiveness of sulfonamides against pneumococci and other organisms. Clostridia and non-sporulating anaerobes grow luxuriantly in this broth when incubated under anaerobic conditions. TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry.For more information visit https://www.neogen.com/solutions/Microbiology/tryptic-soy-broth
NEOGEN UVM Broth, 5 kg
UVM Broth is used for the selective enrichment of Listeria spp and is not intended for use in the diagnosis of disease or other conditions in humans.UVM Broth is a modification of the formula described by Donnelly and Baigent. This formula is used for the selective enrichment of Listeria spp. from food samples. Listeria spp. grow over a pH range of 5.0 - 9.6 and survive in food products with pH levels outside these parameters. Listeria spp. are microaerophilic, Gram-positive, asporogenous, non-encapsulated, non-branching, short, motile rods. Motility is pronounced at 20°C. Identification of Listeria is based on successful isolation of the organism, biochemical characterization, and serological confirmation.For more information visit https://www.neogen.com/solutions/Microbiology/uvm-broth
NEOGEN Sabouraud Dextrose Agar, 5 kg
Sabouraud Dextrose Agar is used for the cultivation of fungi. Conforms to Harmonized USP/EP/JP performance requirements and is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized Pharmacopeia for the isolation and identification of Candida albicans from non-sterile samples. Conforms to USP/EP/JP performance specification. The medium is commonly abbreviated to SDA. The medium is also used for the preparation and maintenance of fungal test strains as described by the Harmonized Pharmacopeia. The peptone digests and dextrose provide a nutritious base for luxuriant fungal growth and the acidic pH affords selectivity against bacteria. Due to the high carbohydrate content and low pH this medium is highly sensitive to overheating which will cause a drop in the gel strength.For more information visit https://www.neogen.com/solutions/Microbiology/sabouraud-dextrose-agar
NEOGEN MacConkey Agar, 10 kg
MacConkey Agar is used for the isolation and differentiation of Gram-negative enteric bacilli in a laboratory setting. Conforms to Harmonized USP/EP/JP Requirements and FDA/BAM. MacConkey Agar is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for isolation and identification of Escherichia coli from non-sterile products. Conforms to USP/EP/JP performance specification. Gelatin serves as source of carbon and nitrogen. Lactose is a fermentable carbohydrate and sodium chloride maintains the osmotic balance. Bile salts and crystal violet act as selective agents inhibiting many Gram-positive bacteria. Escherichia coli can ferment lactose to produce acid which results in a pH drop. This is indicated by neutral red resulting in pink colonies.For more information visit https://www.neogen.com/solutions/Microbiology/macconkey-agar
NEOGEN Violet Red Bile Glucose Agar (VRBGA), 5 kg
Violet Red Bile Glucose Agar is used for the enumeration of Enterobacteriaceae in foods. Violet Red Bile Glucose Agar conforms to Harmonized USP/EP/JP requirements and is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for isolation and identification of bile-tolerant Gram-negative bacteria from non-sterile products. Conforms to Harmonized USP/EP/JP performance specification. Yeast extract provides vitamins and gelatin serves as source of carbon and nitrogen. Glucose is a fermentable carbohydrate and sodium chloride maintains the osmotic balance. Bile salts and crystal violet act as selective agents inhibiting many Gram-positive bacteria. The formulation is a modification of Violet Red Bile Agar by Mossel which substitutes lactose for glucose.For more information visit https://www.neogen.com/solutions/Microbiology/violet-red-bile-glucose-agar
NEOGEN Lactose Broth, 5 kg
Lactose Broth is used for the cultivation of Salmonella and coliform bacteria from food, dairy, and water products in a laboratory setting. Lactose Broth is not intended for use in the diagnosis of disease or other conditions in humans. Lactose Broth is frequently used as a pre-enrichment medium when testing foods and dairy products for Salmonella spp. In dried or processed foods, Salmonella species may be sub-lethally injured and in low numbers. The presence of other bacteria as well as components of the food sample may hinder growth and recovery of Salmonella. Pre-enrichment in a non-selective medium such as Lactose Broth allows for repair of cell damage, dilutes toxic or inhibitory substances, and provides a nutritional advantage to Salmonella over other bacteria.For more information visit https://www.neogen.com/solutions/Microbiology/lactose-broth
NEOGEN Enterobacteria Enrichment (EE) Broth Mossel, 5 kg
Enterobacteria Enrichment (EE) Broth Mossel is used for the cultivation and enrichment of Enterobacteriaceae in food. Enterobacteria Enrichment (EE) Broth Mossel, conforms to Harmonized USP/EP/JP Requirements and is not intended for use in the diagnosis of disease or other conditions in humans. Enterobacteria Enrichment Broth Mossel was developed by Mossel, Visser, and Cornelissen to facilitate the growth of Enterobacteriaceae. This medium contains dextrose to enhance the growth of E. coli and Salmonella spp., particularly in food samples. Nuisance organisms are suppressed by the addition of Ox Bile and Brilliant Green. Enterobacteria Enrichment Broth Mossel is used as an enrichment broth, providing a rich environment for the recovery of damaged or injured cells.For more information visit https://www.neogen.com/solutions/Microbiology/enterobacteria-enrichment-broth-mossel