Additional Selective and Differential Cell Culture Media
NEOGEN Lactobacilli MRS Broth, 500 g
Lactobacilli MRS Broth is used for the cultivation of lactobacilli and is not intended for use in the diagnosis of disease or other conditions in humans.Lactobacilli MRS broth was originally developed in 1960 by de Man, Rogosa & Sharpe, the medium can be used for confirmatory tests on organisms isolated on MRS Agar. The medium can also be used for enumeration by the Miles and Misra technique.Nutrition is provided by a mixture of carefully selected peptones, glucose, beef and yeast extracts while Tween 80, magnesium and manganese sulfates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate.For more information visit https://www.neogen.com/solutions/Microbiology/lactobacilli-mrs-broth
NEOGEN WL Nutrient Agar, 500 g
W-L Nutrient Agar is used for the cultivation of yeasts, molds, and bacteria encountered in brewing and industrial fermentations. W-L Nutrient Agar is not intended for use in the diagnosis of disease or other conditions in humans. W-L Nutrient Medium was developed by Green and Gray while studying various fermentation processes. An exhaustive study examining methods of fermentation control procedures in worts, beers, liquid yeasts and similar fermentation products led to the development of W-L Nutrient Medium. At a pH of 5.5, counts of viable baker’s yeast will grow on W-L Nutrient Medium. W-L Nutrient Medium is also referred to as “Wallerstein Laboratory Medium”.For more information visit https://www.neogen.com/solutions/Microbiology/wl-nutrient-agar
NEOGEN Lauryl Sulfate Broth, 500 g
Lauryl Sulfate Broth is used for the detection of coliform bacteria in water and wastewater in a laboratory setting. Lauryl Sulfate Broth is not intended for use in the diagnosis of disease or other conditions in humans.The coliform group of bacteria includes aerobic and facultative anaerobic, Gram-negative, non-sporeforming bacilli that ferment lactose and form acid and gas at 35°C within 48 hours. Members of the Enterobacteriacae comprise the majority of this group, but organisms such as Aeromonas spp. may also be included. Procedures to detect and confirm coliforms are used in testing water, foods, dairy products, and other materials.Lauryl Sulfate Broth, also referred to as Lauryl Tryptose Broth, is prepared according to the formula of Mallmann and Darby. During their investigation, Sodium Lauryl Sulfate produced the best results for inhibition of organisms other than coliforms. For more information visit https://www.neogen.com/solutions/Microbiology/lauryl-sulfate-broth
NEOGEN Baird-Parker Agar, 500 g
Baird-Parker Agar is used for detection and enumeration of Staphylococcus aureus in foods and is not intended for use in the diagnosis of disease or other conditions in humans.Baird-Parker Agar was first described in 1962. It is a selective medium for the isolation and presumptive identification of coagulase-positive staphylococci. This medium is used extensively for detecting Staphylococcus aureus in foods, dairy products, and other materials. Coagulase-positive staphylococci can grow and reproduce in cosmetic products. These products are tested for the presence of coagulase-positive staphylococci using standard microbiological methods.Formula:Enzymatic Digest of Casein - 10.0 g/LBeef Extract - 5.0 g/LYeast Extract - 1.0 g/LLithium Chloride - 5.0 g/LGlycine - 12.0 g/LSodium Pyruvate - 10.0 g/LAgar - 17.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/baird-parker-agar
NEOGEN Cetrimide Agar
Cetrimide Agar is used in the isolation and identification of Pseudomonas aeruginosa and is not intended for use in the diagnosis of disease or other conditions in humans. Conforms to Harmonized USP/EP/JP Requirements. A medium recommended by the Harmonized USP/EP/JP for the isolation and identification of Pseudomonas aeruginosa, in non- sterile pharmaceutical samples. Conforms to USP/EP/JP performance specification. Gelatin is a source of nitrogen while glycerol acts as a carbon source. Cetrimide is a quaternary ammonium compound that inhibits the growth of a wide range of Gram-positive and some Gram-negative micro-organisms. Magnesium chloride and Dipotassium sulphate improve the production of pyoverdin and pyocyanin pigments that combine to give Pseudomonas aeruginosa characteristic green colonies.For more information visit https://www.neogen.com/solutions/Microbiology/cetrimide-agar
NEOGEN Columbia Blood Agar Base, 500 g
Columbia Blood Agar Base is used with blood for the isolation and cultivation of a wide variety of fastidious microorganisms. Columbia Blood Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. A modification of the original Columbia Agar base formulation, Columbia Blood Agar Base provides a medium that is suitable for use with both defibrinated horse or sheep blood. The peptone mixture provides a source of nitrogen, essential vitamins and amino acids. The starch provides a carbon source and sodium chloride maintains osmotic balance. Originally described as a general purpose nutritious agar base by Ellner et al. Columbia Agar is more frequently used when enriched by the addition of sterile blood. The medium is suitable for supporting the growth and determining hemolytic reactions of a variety of microorganisms. For more information visit https://www.neogen.com/solutions/Microbiology/columbia-blood-agar-base
NEOGEN Maximum Recovery Diluent (Tryptone Salt Broth), 500 g
Maximum Recovery Diluent (Tryptone Salt Broth) is used as an isotonic diluent for maximum recovery of microorganisms and is not intended for use in the diagnosis of disease or other conditions in humans. An osmotically controlled solution which is an alternative to, and a replacement for, 1/4 strength Ringer’s Tablets (NCM0191K). The presence of a low level of peptone lessens the physiological shock normally experienced by bacterial cells when they are introduced to a diluent such as Ringer’s Solution. The level of peptone is such that multiplication of the organisms is not possible in the time in which the sample will be present in the diluent (1-2 hours). This formula is recommended by ISO 6887-1:2017 For more information visit https://www.neogen.com/solutions/Microbiology/maximum-recovery-diluent-tryptone-salt-broth
NEOGEN Universal Pre-enrichment Broth, 500 g
Universal Pre-enrichment Broth is used for the recovery of Salmonella spp. and Listeria spp. in a laboratory setting. Universal Pre-enrichment Broth is not intended for use in the diagnosis of disease or other conditions in humans. Traditional methods for recovering Salmonella spp. and Listeria spp. from food products require separate pre-enrichment media for each microorganism. Some broth media recommended for preenrichment contain antibiotic inhibitors or have insufficient buffering capacity which hinder recovery of sublethally injured cells. Bailey and Cox formulated Universal Preenrichment Broth to permit simultaneous resuscitation of sublethally injured Salmonella and Listeria. The broth medium provides sufficient buffering capacity to prevent rapid decreases in pH and allows for repair of injured cells that might be sensitive to low pH values or inhibitory substances.For more information visit https://www.neogen.com/solutions/Microbiology/universal-preenrichment-broth
NEOGEN Bismuth Sulphite Agar, 500 g
Bismuth Sulfite Agar is used for the selective isolation of Salmonella spp. In a laboratory setting. Bismuth Sulfite Agar is not intended for use in the diagnosis of disease or other conditions in humans and conforms to FDA/BAM formulation. Salmonellosis can result from consumption of raw, undercooked, or improperly processed foods contaminated with Salmonella. U. S. federal guidelines require various poultry products to be routinely monitored before distribution for human consumption. Bismuth Sulfite Agar is a modification of Wilson and Blair formula. The typhoid organism grows luxuriantly on the medium, forming characteristic black colonies. Gram-positive bacteria and coliforms are inhibited on Bismuth Sulfite Agar. The inhibitory action of Bismuth Sulfite Agar permits the use of a large inoculum, increasing the possibility of recovering pathogens that may be present in small numbers.For more information visit https://www.neogen.com/solutions/Microbiology/bismuth-sulfite-agar
NEOGEN Tryptic Soy Broth (Soybean-Casein Digest Broth), 500 g
Tryptic Soy Broth (Soybean-Casein Digest Broth) is used for the cultivation of a wide variety of microorganisms. Tryptic Soy Broth conforms to Harmonized USP/EP/JP requirements and is not intended for use in the diagnosis of disease or other conditions in humans.Tryptic Soy Broth, a general purpose medium, is commonly referred to as Casein Soya Bean or Soybean-Casein Digest broth, and abbreviated as TSB. This medium was originally developed for use without blood in determining the effectiveness of sulfonamides against pneumococci and other organisms. Clostridia and non-sporulating anaerobes grow luxuriantly in this broth when incubated under anaerobic conditions. TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry.For more information visit https://www.neogen.com/solutions/Microbiology/tryptic-soy-broth
NEOGEN Tryptone Soy Agar, 500 g
Tryptone Soy Agar (TSA) is a general-purpose agar which will support the growth of a wide range of micro-organisms, and is not intended for use in the diagnosis of disease or other conditions in humans.The medium can be used for phage typing, colicine typing and for testing the X and V factor requirements of Haemophilus spp.Formula:Tryptone - 15.0 g/LSoy Peptone - 0.5 g/LSodium Chloride - 5.0 g/LAgar - 12.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/tryptone-soy-agar
NEOGEN Lauryl Sulfate Broth with MUG, 500 g
Lauryl Sulfate Broth with MUG is used for the detection of coliforms and the fluorogenic detection of Escherichia coli in a laboratory setting. Lauryl Sulfate Broth with MUG is not intended for use in the diagnosis of disease or other conditions in humans.The coliform group of bacteria includes aerobic and facultative anaerobic, Gram-negative, non-sporeforming bacilli that ferment lactose and form acid and gas at 35°C within 48 hours. Members of the Enterobacteriacae comprise the majority of this group, but organisms such as Aeromonas spp. may also be included. Procedures to detect and confirm coliforms are used in testing water, foods, dairy products, and other materials.Lauryl Sulfate Broth, also referred to as Lauryl Tryptose Broth, is prepared according to the formula of Mallmann and Darby.For more information visit https://www.neogen.com/solutions/Microbiology/lauryl-sulfate-broth-with-mug
NEOGEN Sabouraud Dextrose Agar, 5 kg
Sabouraud Dextrose Agar is used for the cultivation of fungi. Conforms to Harmonized USP/EP/JP performance requirements and is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized Pharmacopeia for the isolation and identification of Candida albicans from non-sterile samples. Conforms to USP/EP/JP performance specification. The medium is commonly abbreviated to SDA. The medium is also used for the preparation and maintenance of fungal test strains as described by the Harmonized Pharmacopeia. The peptone digests and dextrose provide a nutritious base for luxuriant fungal growth and the acidic pH affords selectivity against bacteria. Due to the high carbohydrate content and low pH this medium is highly sensitive to overheating which will cause a drop in the gel strength.For more information visit https://www.neogen.com/solutions/Microbiology/sabouraud-dextrose-agar
NEOGEN Xylose Lysine Deoxycholate (XLD) Agar (ISO), 500 g
Xylose Lysine Deoxycholate (XLD) Agar (ISO) is a selective agar for the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. Xylose Lysine Deoxycholate (XLD) Agar (ISO) is not intended for use in the diagnosis of disease or other conditions in humans. A selective, diagnostic agar for the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. Originally formulated by Taylor to differentiate enteric pathogens, the agar is widely used as the preferred differential medium for Salmonella spp. The medium is void of peptones but instead uses yeast extract as a carbon, nitrogen and vitamin source and xylose, lactose and sucrose are fermentable carbohydrates.For more information visit https://www.neogen.com/solutions/Microbiology/xylose-lysine-deoxycholate-xld-agar-iso
NEOGEN Violet Red Bile Glucose Agar (VRBGA), 500 g
Violet Red Bile Glucose Agar is used for the enumeration of Enterobacteriaceae in foods. Violet Red Bile Glucose Agar conforms to Harmonized USP/EP/JP requirements and is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for isolation and identification of bile-tolerant Gram-negative bacteria from non-sterile products. Conforms to Harmonized USP/EP/JP performance specification. Yeast extract provides vitamins and gelatin serves as source of carbon and nitrogen. Glucose is a fermentable carbohydrate and sodium chloride maintains the osmotic balance. Bile salts and crystal violet act as selective agents inhibiting many Gram-positive bacteria. The formulation is a modification of Violet Red Bile Agar by Mossel which substitutes lactose for glucose.For more information visit https://www.neogen.com/solutions/Microbiology/violet-red-bile-glucose-agar