Cell Lines and Cell Cultures
Eurofins Discoverx ChemiBrite IP1 Prostanoid Receptor Stable Cell Line, 2 vials. (HTS131L)
The ChemiBrite IP1 Prostanoid Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin I2) based activation of PTGIR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGIR, a naturally Gq coupled GPCR. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGIR, the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen APJ Apelin Receptor Stable Cell Line, 2 vials. (HTS068C)
The ChemiScreen APJ Apelin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. apelin) based activation of AGTRL1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses AGTRL1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of AGTRL1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay PTH1 Parathyroid Hormone Receptor Frozen Cells, 2 Vials of Cells. (HTS030RTA)
The Ready-to-Assay PTH1 Parathyroid Hormone Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. parathyroid hormone) based activation of PTH1R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTH1R alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PTH1R, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen V2 Vasopressin Receptor Membrane Preparation, 1 vial. (HTS060M)
The ChemiScreen V2 Vasopressin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of AVPR2 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiBrite CysLT1 Leukotriene Receptor Stable Cell Line, 2 vials. (HTS061L)
The ChemiBrite CysLT1 Leukotriene Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. leukotriene D4) based activation of CysLT1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CysLT1, a naturally Gq coupled GPCR. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of CysLT1, the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
ATCC Clonostachys rosea
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
ATCC Listeria monocytogenes; Strain Li 23
Listeria monocytogenes strain Li 23 was isolated from animal tissue and has applications in bioinformatics, enteric disease research, infectious disease research, and zoonotic disease research.
Eurofins Discoverx ChemiScreen mGlu2 Metabotropic Glutamate Receptor Stable Cell Line, 2 vials. (HTS146C)
The ChemiScreen mGlu2 Metabotropic Glutamate Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. glutamate) based activation of GRM2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GRM2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of GRM2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay S1P2 Lysophospholipid Receptor Frozen Cells, 2 Vials of Cells. (HTS078RTA)
The Ready-to-Assay S1P2 Lysophospholipid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. S1P) based activation of S1PR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses S1PR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of S1PR2, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen CCR3 Chemokine Receptor Stable Cell Line, 2 vials. (HTS008C)
The ChemiScreen CCR3 Chemokine Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. eotaxin) based activation of CCR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CCR3 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CCR3, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen CCR2B Receptor Stable Cell Line, 2 vials. (HTS007C)
The ChemiScreen CCR2B Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CCL2, MCP-1) based activation of CCR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CCR2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CCR2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen AT1 Angiotensin Receptor Membrane Preparation, 1 vial. (HTS064M)
The ChemiScreen AT1 Angiotensin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of AGTR1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.