Cell Lines and Cell Cultures
Genscript Corporation CHO-K1/BTLA Stable Cell Line
The B and T lymphocyte attenuator (BTLA) is an Ig super family protein with an intermediate type Ig fold in the ectodomain and an ITIM inhibitory signaling domain in the cytosol BTLA interacts with the herpesvirus entry mediator (HVEM TNFRSF14) a TNFR super family member Engagement of BTLA by HVEM induces tyrosine phosphorylation of the ITIM motifs in the cytoplasmic tail of BTLA allowing the recruitment of the phosphatases SHP-1 and SHP-2 which attenuate signaling
Genscript Corporation CHO-K1/B7-H4 Stable Cell Line
B7-H4 is a transmembrane protein that binds an unknown receptor on activated T cells resulting in inhibition of T-cell effector function via cell cycle arrest decreased proliferation and reduced IL-2 production B7-H4 is up-regulated on the surface of cancer cells and immunosuppressive tumor-associated macrophages (TAMs) in a variety of human cancers
Genscript Corporation CHO-K1/CCKA/G15 Stable Cell L
Cholecystokinin (CCK)-A and CCK-B receptors are highly homologous members of the seven transmembrane domain G-protein-coupled receptor super-family Peptides in the cholecystokinin (CCK) family have a variety of biological functions in the central and peripheral nervous systems as well as in the gastrointestinal tract The CCKA receptor has a more limited distribution with the highest densities in the hypothalamic nuclei areas of the hippocampus the septum dorsal motor vagal nucleus and interpeduncular nucleus of the brain stem It also occurs in numerous gastro-intestinal tissues Binding of ligands to CCK1 stimulates mobilization of intracellular calcium by activation of Gq/11 CCKA receptors affect satiety pancreatitis and gut motility and have growth-promoting effects on some tumors
Genscript Corporation CHO-K1/NPY4/G15 Stable Cell L
Recombinant CHO-K1 cells stably overexpress human neuropeptide Y receptor Y4 (NPY4) on the surface and contain high levels of G protein G i and G q to couple with the receptor in downstream signaling pathways
Genscript Corporation CHO-K1/NMU1 Stable Cell Line
Neuromedin U receptor 1 (NMUR1) is a member of G-protein coupled receptor family A and is the most abundant in peripheral tissue particularly in small intestines and stomach This protein is a receptor for Neuromedin U (NMU-25) The first biological activity ascribed to NmU was smooth muscle contraction Neuromedin U has also been reported to increase arterial blood pressure and modify ion transport in the intestinal tract Finally NmU injected subcutaneously into rats has been reported to result in a short term increase in circulating ACTH levels and a long term increase in serum corticosterone levels suggesting a role in regulation of the hypothalamo-pituitary-adrenal axis
Genscript Corporation CD47 Fc Chimera Human
Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3 Integrin-associated protein (IAP) and Protein MER6 CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain CD47 is a 40 60 kDa variably glycosylated atypical member of the immunoglobulin superfamily and an integral membrane protein that consists of a 123 amino acid (aa) extracellular domain (ECD) with a single Ig-like domain five membrane-spanning regions with short intervening loops and a 34 aa C-terminal cytoplasmic tail CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity CD47 is the receptor for SIRPA binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells CD47 Interaction with SIRPG mediates cell-cell adhesion enhances superantigen-
Bioss Dopamine D3 receptor Monoclona
Dopamine D3 receptor Primary Antibody Unconjugated Host Species Rabbit Target Species Human Mouse Rat Clonality Monoclonal Applications WB RecombinantConcentration 1ug/ul Size 100 ul Storage Buffer 10mM phosphate buffered saline(pH 7 4) with 150mM sodium chloride 0 05% BSA 0 02% sodium azide and 50% glycerol
Bioss KD-Validated Insulin receptor
Insulin Receptor Primary Antibody Unconjugated Public Immunogen Range 910-976/1382 Host Species Rabbit Target Species Human Mouse Rat Clonality Monoclonal Applications WB FCM Concentration Lot dependent Size 50 ul Storage Buffer Supplied in PBS (pH 7 4) containing 50% glycerol and 0 02% sodium azide
Bioss Folate Receptor alpha Monoclon
Folate Receptor alpha Primary Antibody Unconjugated Host Species Rabbit Target Species Human Mouse Rat Clonality Monoclonal Applications WB RecombinantConcentration 1ug/ul Size 100 ul Storage Buffer 10mM phosphate buffered saline(pH 7 4) with 150mM sodium chloride 0 05% BSA 0 02% sodium azide and 50% glycerol
Bioss Endothelin B Receptor Monoclon
Endothelin B Receptor Primary Antibody Unconjugated Host Species Rabbit Target Species Human Clonality Monoclonal Applications WB IP RecombinantConcentration 1ug/ul Size 100 ul Storage Buffer 10mM phosphate buffered saline(pH 7 4) with 150mM sodium chloride 0 05% BSA 0 02% sodium azide and 50% glycerol
AllCells LLC Peripheral Blood, Cryopreserved, Mononuclear Cells (PBMC), 50M
Peripheral blood cells collected by apheresis are treated with ammonium chloride to remove red blood cells (RBCs) and washed to remove platelets. Viability is 90%. Our peripheral blood MNC products (PBMCs) are composed of, on average, 50% T cells, 20% monocytes, 10% B cells, 7% NK cells, and 3% granulocytes. Actual cell type percentages vary from collection to collection.
AllCells LLC AllCells Human Healthy Donor Peripheral Blood Leukopak, Fresh, 5 billion cells minimum
Leukapheresis is performed using the Spectra Optia Apheresis System. Mononucleated cell-rich blood is continuously collected using ACD-A as an anticoagulant. This product provides no less than 5 billion total nucleated cells (TNC). TNC in our leukapheresis products are composed of, on average, 50% T cells, 20% monocytes, 10% B cells, 7% NK cells, and 3% granulocytes. Actual product volume, TNC concentration, and cell type percentages vary from collection to collection.
HLA and CMV status are available upon request. All donors undergo an in-person screening including bloodborne pathogens testing, Hepatitis B, Hepatitis C, and HIV 1 and 2.
AllCells boasts the largest donor database in the industry. Our pool of healthy, diverse and recallable donors provides researchers with the right cells at the right time. AllCells donors undergo initial in-depth screening, followed by regular re-screening at three month intervals.
Genscript Corporation CHO-K1/TIGIT Stable Cell Line
TIGIT (T cell immunoreceptor with Ig and ITIM domains) was recently characterized as an inhibitory receptor which is expressed mainly on NK Treg CD8 T and CD4 T cells TIGIT harbors an immunoglobulin tail tyrosine (ITT)-like phosphorylation motif and an ITIM (immunoreceptor tyrosine-based inhibition motif) in its cytoplasmic tail The poliovirus receptor (PVR or CD155) was identified as the physical ligand of TIGIT with high affinity and PVRL2 (Nectin2 or CD112) also binds to TIGIT with a weaker binding capacity TIGIT/PVR engagement suppresses T cell activation through IL-10 secretion mediated by dendritic cells Furthermore TIGIT also exerts an intrinsic inhibitory function to T cell activation
Genscript Corporation CHO-K1/cyno 4-1BB Stable Cell
4-1BB is a member of the tumor necrosis factor (TNF) receptor family Its alternative names are tumor necrosis factor receptor superfamily member 9 (TNFRSF9) CD137 and induced by lymphocyte activation (ILA) It is currently of interest to immunologists as a co-stimulatory immune checkpoint molecule 4-1BB can be expressed by activated T cells but to a larger extent on CD8 than on CD4 T cells In addition 4-1BB expression is found on dendritic cells follicular dendritic cells natural killer cells granulocytes and cells of blood vessel walls at sites of inflammation
Genscript Corporation CHO-K1/BDCA2 and FcER1G Stable
Recombinant CHO-K1 cells stably overexpress human C-type lectin domain family 4 member C (BDCA2) and Fc epsilon receptor Ig (FcER1G) on the surface The surface expression of BDCA2 is validated by FACS analysis