Cell Lines and Cell Cultures
Eurofins Discoverx Ready-to-Assay CXCR2 Chemokine Receptor Frozen Cells, 2 Vials of Cells. (HTS002RTA)
The Ready-to-Assay CXCR2 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. CXCL8/IL8) based activation of CXCR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CXCR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CXCR2, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay GIP Glucagon Receptor Frozen Cells, 2 Vials of Cells. (HTS134RTA)
The Ready-to-Assay GIP Glucagon Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. GIP) based activation of GIPR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GIPR alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of GIPR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen IP1 Prostanoid Receptor Stable Cell Line, 2 vials. (HTS131C)
The ChemiScreen IP1 Prostanoid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin I2) based activation of PTGIR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGIR alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of PTGIR, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen Rhesus Macaque CCR5 Chemokine Receptor Stable Cell Line, 2 vials. (HTS010C)
The ChemiScreen Rhesus Macaque CCR5 Chemokine Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. MIP, RANTES) based activation of CCR5 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CCR5 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CCR5, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the rhesus macaque version of the protein. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay CCR1 Chemokine Receptor Frozen Cells, 2 Vials of Cells. (HTS005RTA)
The Ready-to-Assay CCR1 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. CCL2, MCP-1) based activation of CCR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CCR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CCR1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen TP Prostanoid Receptor Stable Cell Line, 2 vials. (HTS081C)
The ChemiScreen TP Prostanoid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin H2, thromboxane A2) based activation of TBXA2R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses TBXA2R alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of TBXA2R, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay BB2 Bombesin Receptor Frozen Cells, 2 Vials of Cells. (HTS084RTA)
The Ready-to-Assay BB2 Bombesin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. bombesin) based activation of GRPR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GRPR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of GRPR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen PAR2 Receptor Stable Cell Line, 2 vials. (HTS037C)
The ChemiScreen PAR2 Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. PAR2) based activation of F2RL1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses F2RL1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of F2RL1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen CCK2 Cholecystokinin Receptor Membrane Preparation, 1 vial. (HTS083M)
The ChemiScreen CCK2 Cholecystokinin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CCKBR surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx Ready-to-Assay AT1 Angiotensin Receptor Frozen Cells, 2 Vials of Cells. (HTS064RTA)
The Ready-to-Assay AT1 Angiotensin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. Angiotensin-II) based activation of AGTR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses AGTR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of AGTR1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen AT1 Angiotesin Receptor Stable Cell Line, 2 vials. (HTS064C)
The ChemiScreen AT1 Angiotensin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. Angiotensin-II) based activation of AGTR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses AGTR1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of AGTR1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay NMU1 Neuromedin U Receptor Frozen Cells, 2 Vials of Cells. (HTS062RTA)
The Ready-to-Assay nMU1 Neuromedin U Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. Neuromedin U) based activation of nMU1R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses nMU1R alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of nMU1R, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen NMU1 Neuromedin U Receptor Stable Cell Line, 2 vials. (HTS062C)
The ChemiScreen nMU1 Neuromedin U Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. Neuromedin U) based activation of nMU1R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses nMU1R alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of nMU1R, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx PrecisION hTRPV1 Recombinant Stable Cell Line, 2 vials. (CYL3063)
The PrecisION hTRPV1 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying TRPV1 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses TRPV1 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Eurofins Discoverx ChemiScreen MC5 Melanocortin Receptor Membrane Preparation, 1 vial. (HTS155M)
The ChemiScreen MC5 Melanocortin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of MC5R surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.