Cell Lines and Cell Cultures
Eurofins Discoverx Ready-to-Assay XCR1 Chemokine Receptor Frozen Cells, 2 Vials of Cells. (HTS053RTA)
The Ready-to-Assay XCR1 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. lymphotactin) based activation of XCR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses XCR1 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of XCR1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen CysLT1 Leukotriene Receptor Stable Cell Line, 2 vials. (HTS061C)
The ChemiScreen CysLT1 Leukotriene Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. leukotriene) based activation of CysLT1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CysLT1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CysLT1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen CX3CR1 Receptor Stable Cell Line, 2 vials. (HTS015C)
The ChemiScreen CX3CR1 Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. fractalkine) based activation of CX3CR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CX3CR1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CX3CR1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen GPR40 Free Fatty Acid Receptor Stable Cell Line, 2 vials. (HTS038C)
The ChemiScreen GPR40 Free Fatty Acid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. free fatty acids) based activation of FFAR1 (GPR40) GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FFAR1 (GPR40) alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of FFAR1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen D2L Dopamine Receptor Stable Cell Line, 2 vials. (HTS039C2)
The ChemiScreen D2L Dopamine Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. dopamine) based activation of DRD2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses DRD2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of DRD2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen V1A Vasopression Receptor Stable Cell Line, 2 vials. (HTS059C)
The ChemiScreen V1A Vasopressin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. vasopressin) based activation of AVPR1A GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses AVPR1A alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of AVPR1A, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen TP Prostanoid Receptor Stable Cell Line, 2 vials. (HTS081C)
The ChemiScreen TP Prostanoid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin H2, thromboxane A2) based activation of TBXA2R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses TBXA2R alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of TBXA2R, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen α1A Adrenergic Receptor Membrane Preparation, 1 vial. (HTS087M)
The ChemiScreen α1A Adrenergic Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of ADRA1A surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen AT1 Angiotensin Receptor Membrane Preparation, 1 vial. (HTS064M)
The ChemiScreen AT1 Angiotensin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of AGTR1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx Ready-to-Assay ETA Endothelin Receptor Frozen Cells, 2 Vials of Cells. (HTS070RTA)
The Ready-to-Assay ETA Endothelin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. endothelin) based activation of EDNRA GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses EDNRA alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of EDNRA, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen CysLT2 Leukotriene Receptor Stable Cell Line, 2 vials. (HTS088C)
The ChemiScreen CysLT2 Leukotriene Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. leukotriene) based activation of CYSLTR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CYSLTR2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CYSLTR2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen OT Oxytocin Receptor Stable Cell Line, 2 vials. (HTS090C)
The ChemiScreen OT Oxytocin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. oxytocin) based activation of OXTR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses OXTR alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of OXTR, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay EP3 Prostanoid Receptor Frozen Cells, 2 Vials of Cells. (HTS092LRTA)
The Ready-to-Assay EP3 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin E2) based activation of PTGER3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER3 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER3, the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay Glucagon Receptor Frozen Cells, 2 Vials of Cells. (HTS112RTA)
The Ready-to-Assay Glucagon Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. glucagon) based activation of GCGR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GCGR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of GCGR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen ChemR23 Chemoattractant Receptor Stable Cell Line, 2 vials. (HTS071C)
The ChemiScreen ChemR23 Chemoattractant Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. chemerin) based activation of CMKLR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CMKLR1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CMKLR1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.