General Cell Lines and Cell Cultures
ATCC Malassezia furfur; M 133/78
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
ATCC Fusarium venenatum
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
ATCC pRS316 phagemid; in E. coli
This is one of a series of pBluescript-based YC-type (centromeric) yeast shuttle vectors (ATCC 77142-77145) differing in the yeast selectable marker gene. It encodes the beta galactosidase alpha peptide for blue-white color detection of inserts and also has the T7 and T3 promoters for in vitro RNA synthesis and priming sites for sequencing. It is useful in plasmid shuffling experiments. It was constructed by inserting a 1.112 kb fragment containing the URA3 gene into the NdeI site of the pRSS56 vector and also inserting a cassette containing CEN6 and the ARS associated with histone 4 (ARSH4) into the AatII site of the same vector. All ends were blunted. The pRSS56 vector was constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ and the fragment from the unique NdeI and AatII sites between bla and f1 origin of pBS(+). The order of the major features in this plasmid is: URA3 ? f1 ori (NaeI) ? T7 promoter ? lacZ?/MCS ? T3 promoter ? pMB1 ori ? bla ? CEN6 ? ARSH4.
ATCC Neisseria meningitidis; serogroup y
Neisseria meningitidis strain M-112 [BO-6] is a whole-genome sequenced bacterial strain.
ATCC Pseudomonas aeruginosa
Pseudomonas aeruginosa strain PRD-10 [CIP 103467, NCIB 10421, PCI 812] is a whole genome-sequenced strain that was isolated from a water bottle in an animal room. This bacterium can be used in disinfectant testing, antimicrobial preservative assays, testing of antimicrobial handwashing formulations, testing of slimicide, and quality control of Biosynth products. It is recommended by ATCC for use in the test described in ASTM Standard Test Method E686-91 where only the taxon is specified.
FUJIFILM CELLULAR DYNAMICS INC CRYOPRESERVED ICELL DOPANEURON
NC3802971 CRYOPRESERVED ICELL DOPANEURON
Maine Molecular Quality Controls Inc QIAstat-Dx GI 2 Control Panel
QIAstat-Dx GI 2 Control Panel is intended for use as reference material to monitor the performance of in vitro laboratory nucleic acid testing procedures for the qualitative detection of bacterial, viral, and parasitic pathogens the QIAstat-Dx Gastrointestinal Panel 2 performed on QIAstat-Dx systems. QIAstat-Dx GI 2 Control Panel is composed of 3 controls, QIAstat-Dx GI 2 Positive A, QIAstat-Dx GI 2 Positive B, and QIAstat-Dx GI 2 Negative. QIAstat-Dx GI 2 Positive A and QIAstat-Dx GI 2 Positive B contain surrogate control material composed of synthetic nucleic acid corresponding to genome segments of pathogens. QIAstat-Dx GI 2 Negative contains no nucleic acid.
Zymo Research Corporation Mix & Go! Competent Cells - DH5 Alpha, 96 x 50 µl
Mix & Go E. coli cells are made chemically competent by a unique method that completely eliminates the need for heat shock and related procedures. For transformation, DNA can be added directly to Mix & Go cells and the mixture spread directly to a culture plate. Transformation efficiencies typically range from 108-109 transformants/µg of pUC19 DNA , which make the cells optimal for cloning, sub-cloning, library construction, etc. Premade Mix & Go cells are supplied as a pack of 10 convenient 100 µl/tube aliquots or in a 96-well format (12 x 8 - tube strips) of 50 µl/tube.
ADDEXBIO TECHNOLOGIES HACAT CELLS (T0020001
In vitro spontaneously transformed keratinocytes from histologically normal skin. Species: Homo sapiens. Keratinocyte. Adherent monolayer. Patient: Male, Caucasian, 62 years of age. Medium: AddexBio-Optimized DMEM (C0003-02) + 10% FBS. Subculture: Recommended 1:5 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degrees Celsius. Bacteria: Negative. Yeast: Negative. Mycoplasma: Negative. HIV: Negative. Hepatitis B: Negative. Hepatitis C: Negative.
Takara Bio Stellar™ Competent Cells, 10 x 100 ul
Stellar Competent Cells are an E. coli HST08 strain that provides high transformation efficiency. These cells can be used in a wide variety of applications—from preparation of cDNA and genomic libraries, to construction of longer-length genomic libraries, to sub-cloning, and even methylated DNA cloning. Stellar Competent Cells lack the gene cluster for cutting foreign methylated DNA (mrr-hsdRMS-mcrBC and mcrA), and are therefore useful for cloning methylated DNA. The cells can also be used for blue/white screening (i.e., α-complementation) when transformed with vectors containing the lacZα gene. A pUC19 vector is provided as well as SOC Medium. Stellar Competent Cells are recommended for use with Clontech's In-Fusion PCR Cloning Kits. 10 x 100 ul
STEMCELL Technologies Human Peripheral Blood Mononuclear Cells, Frozen
Streamline your assays with ready-to-use, ethically sourced, primary human mononuclear cells. With personalized service, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications, we help you get the cells you need. Isolated from peripheral blood leukapheresis samples using density gradient separation and/or red blood cell lysis and cryopreserved in animal component-free CryoStorCS10 medium (Catalog #07930), cells are collected using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Acid-citrate-dextrose solution A (ACDA) is added during collection as an anticoagulant. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.Certain products are only available in select territories.
Lonza Walkersville Gentamicin sulfate-Amphotericin (GA-1000) , 5 mL
GA-1000 vial contains 30 mg/ml Gentamicin and 15 µg/ml Amphotericin. We recommend use at a 1:1000 ratio (i.e. 0.2 ml into a 200 ml bottle for a final concentration of 30 µg/ml Gentamicin and 15 ng/ml Amphotericin. GA-1000 is included in all Clonetics Primary Cell Culture Media Kits. This product is not suitable for human or veterinary use.Content:1 Vial Containing 5 mL GA-1000. 30 mg/mL Gentamicin and 15 µg/mL Amphotericin