General Cell Lines and Cell Cultures
Clontech Labs 3P Stellar™ Competent Cells, 10 x 100 ul
Stellar Competent Cells are an E. coli HST08 strain that provides high transformation efficiency. These cells can be used in a wide variety of applications—from preparation of cDNA and genomic libraries, to construction of longer-length genomic libraries, to sub-cloning, and even methylated DNA cloning. Stellar Competent Cells lack the gene cluster for cutting foreign methylated DNA (mrr-hsdRMS-mcrBC and mcrA), and are therefore useful for cloning methylated DNA. The cells can also be used for blue/white screening (i.e., α-complementation) when transformed with vectors containing the lacZα gene. A pUC19 vector is provided as well as SOC Medium. Stellar Competent Cells are recommended for use with Clontech's In-Fusion PCR Cloning Kits. 10 x 100 ul
STEMCELL Technologies Human Peripheral Blood Mononuclear Cells, Frozen
Streamline your assays with ready-to-use, ethically sourced, primary human mononuclear cells. With personalized service, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications, we help you get the cells you need. Isolated from peripheral blood leukapheresis samples using density gradient separation and/or red blood cell lysis and cryopreserved in animal component-free CryoStorCS10 medium (Catalog #07930), cells are collected using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Acid-citrate-dextrose solution A (ACDA) is added during collection as an anticoagulant. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.Certain products are only available in select territories.
STEMCELL Technologies Human Peripheral Blood NK Cells, Frozen, Size: 5 x 10^6 cells
Primary human NK cells were isolated from peripheral blood (PB) mononuclear cells (MNCs) using negative immunomagnetic separation techniques. PB was collected using acid-citrate-dextrose solution A (ACDA) as the anticoagulant.Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.Certain products are only available in select territories. Please contact your local Sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.Browse our Frequently Asked Questions (FAQs) (https://www.stemcell.com/primary-cells-faq) on Primary Cells.
iQ Biosciences BALB/c Mouse Splenocytes, 25M cells/vial
High-quality, high-viability (typically > 70%) splenocytes from the BALB/c mouse, a commonly-used inbred mouse strain. Used for a wide variety of immunology-based applications, including in-vitro immunoassays and ex-vivo applications. All orders come with an iQ Certificate of Analysis.
Anaerobe Systems BRU, LKV, PEA - Brucella Blood Agar, Laked Brucella Blood Agar with Kanamycin and Vancomycin, Phenylethyl Alcohol Blood Agar, 3 Plates/Pkg
This item has a minimum qty of 5 per supplier requirements.
3-plate convenience package containing 1 each of Brucella Blood Agar (BRU), Phenylethyl Alcohol Agar (PEA), and Laked Brucella Blood Agar w/ Kanamycin and Vancomycin (LKV) in a single package. Media are prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.BRU, LKV, and PEA media may be utilized in the initial set up of clinical specimens to aid in the isolation and identification from mixed flora samples. BRU is a non-selective general enrichment medium supporting broad spectrum anaerobic growth. PEA is a selective medium that inhibits facultative gram-negative rods and swarming of some Clostridium & Proteus species. LKV is an enriched, selective, and differential medium for the isolation and partial identification of obligately anaerobic gram-negative bacilli.
Lonza Walkersville SKGM - 2 Skeletal Muscle Cell Growth Medium-2 SingleQuots Supplements and Growth Factors
SkGM -2 SingleQuot Kit are components sold separately and does not include the basal medium. These are part of the SkGM -2 Skeletal Muscle Myoblast Medium BulletKit (Catalog Number CC-3245). The full SkGM -2 BulletKit consisting of basal medium and supplements can be purchased together using the catalog number CC-3245. We offers two varieties of Skeletal Muscle Growth Medium - SkGM and SkGM-2.SkGM-2 Skeletal Muscle Cell Growth Medium-2 is an enhancement of SkGM Medium. Fetuin has been removed and there has been further optimization of the basal media.SkGM Medium is optimized for use with human skeletal muscle cells. SkGM -2 is specially designed for use with normal and diabetic skeletal muscle myoblasts.Content:1 x Red Cap Vial with GA-1000 0.50 mL1 x Green Cap Vial with hEGF 0.50 mL1 x Natural Cap Vial with Dexamethasone 0.50 mL1 x Bottle with FBS 50.00 mL1 x Bottle L-Glutamine 10.00 mL
Promocell GmbH PromoCell hMDM-GMCSF(-)-c 1,5 million Human M1 Macrophages (GM-CSF), Monocyte-derived, single donor, cryopreserved
PromoCell's cryopreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.Cryopreserved human macrophages are produced from monocytes in PromoCell's well-proven M1 Macrophage Generation Media XF and are available as non-activated, fully qualified M1- (GM-CSF) polarized cells. After 9-10 days of differentiation, the M1 macrophages are cryopreserved using PromoCells's proprietary, defined and animal-free freezing medium, Cryo-SFM. Each cryo vial contains more than 1.5 million or 5 million viable cells after thawing, respectively.Our M1 Macrophages are now also available from HLA-typed donors.
Leinco Technologies Inc 1X ClearFlow™ Sheath Fluid | 20L
ClearFlow™ Sheath Fluid is specifically formulated isotonic solution for transporting particles through a flow cytometer and supplied "Ready to Use". ClearFlow™ is specially manufactured in our ISO 7(class 10,000) cleanroom suite for low particulate count (SEE ABOVE EXPERIMENT) and fluorescence background to guarantee better signal to noise ratio measurements.
Sigma Aldrich Fine Chemicals Biosciences PKH26 Red Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling Distributed for Phanos Technologies | 1KT
PKH26 Red Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling Distributed for Phanos Technologies | 1KT
iQ Biosciences Beagle Canine PBMCs, 10M cells/vial
High-quality, high-viability (> 80%) peripheral blood mononuclear cells (PBMC) from the beagle canine, a commonly-used dog breed in research. Used for a wide variety of immunology-based applications, including in-vitro immunoassays and ex-vivo applications. All orders come with an iQ Certificate of Analysis.
ADDEXBIO TECHNOLOGIES HACAT CELLS (T0020001
In vitro spontaneously transformed keratinocytes from histologically normal skin. Species: Homo sapiens. Keratinocyte. Adherent monolayer. Patient: Male, Caucasian, 62 years of age. Medium: AddexBio-Optimized DMEM (C0003-02) + 10% FBS. Subculture: Recommended 1:5 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degrees Celsius. Bacteria: Negative. Yeast: Negative. Mycoplasma: Negative. HIV: Negative. Hepatitis B: Negative. Hepatitis C: Negative.
Agilent Technologies BL21-CODON PLUS RIPL CELLS
BL21-CodonPlus (DE3)-RIPL Competent Cells, 10 x 0.1mL, These cells contain extra copies of rare E. coli argU, ileY, leuW, proL tRNA genes which corrects for codon bias and dramatically improves expression of sequences from other organisms