General Cell Lines and Cell Cultures
Eurofins Discoverx Ready-to-Assay CALCRL Calcitonin Receptor Frozen Cells, 2 Vials of Cells. (HTS172RTA)
The Ready-to-Assay CALCRL Calcitonin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. parathyroid hormone) based activation of CALCRL GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CALCRL (and modulating protein RAMP1) alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CALCRL, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx PathHunter U2OS SYK Activity Assay, 2 vials. (93-0865C3)
The PathHunter U2OS SYK Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting the SYK kinase domain. The cell line is engineered to express the catalytic domain from SYK fused to the cytokine receptor binding domain (CRBD) of JAK2. The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the GHR-PK. Activation of the GHR-PK induces receptor phosphorylation by the SYK catalytic domain. This leads to SH2-EA recruitment and production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx Ready-to-Assay MC5 Melanocortin Receptor Frozen Cells, 2 Vials of Cells. (HTS155RTA)
The Ready-to-Assay MC5 Melanocortin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. α-MSH) based activation of MC5R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MC5R alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of MC5R, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen MC5 Melanocortin Receptor Membrane Preparation, 1 vial. (HTS155M)
The ChemiScreen MC5 Melanocortin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of MC5R surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Genscript Corporation CHO-K1/ADRA1B Stable Cell Line
Recombinant CHO-K1 cells stably overexpress human adrenoceptor alpha 1B (ADRA1B) on the surface and contain high levels of G protein G q to couple with the receptor in downstream signaling pathways
Lonza Walkersville D-Hu Subcut Preadipo Diabet II
Poietics Diseased Human Preadipocytes (D-HprAD) are isolated from subcutaneous or visceral fat from diabetic Type I and diabetic Type II donors Preadipocytes from normal (non-diabetic) donors are available as a separate product Pertinent additional donor information including Body Mass Index (BMI) insulin dependence and age of diagnosis may be available by contacting Scientific Support Cryopreserved Poietics Diseased Human Preadipocytes (D-HprAD) are guaranteed to contain 1 million cells per ampoule and test positive for adipogenic differentiation All cells test negative for mycoplasma bacteria yeast and fungi HIV-1 hepatitis B and hepatitis C are not detected for all donors and/or cell lots A Certificate of Analysis is provided for each cell lot purchased
ATCC hTERT ipNF95.11b C; Plexiform neurofibroma; Human (Homo sapiens)
For-profit organizations: For every order of this item, you must provide a signed Material Transfer Agreement Addendum for Screening Application. We cannot ship this item until we receive this addendum. The person signing the addendum as the principal investigator must match the end user as listed on the applicable sales order for the item.Email the signed addendum to SalesPermits@atcc.org with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. Additional fees may apply if this product is being used for a screening use (ATCC ACS-2103F), and these fees will be applied after your order is confirmed. If you need assistance with your order, please contact our Customer Care team.
ATCC Methylorubrum extorquens; (u s patent 5,302,525)
Methylorubrum extorquens strain 58 is a bacterium that was isolated from oil-contaminated soil. It is cited to produce poly-beta-hydroxybutyric acid polymers.
Biochain Institute Inc Paraffin Tissue Section - Human Throat Tumor: Squamous Cell Carcinoma of Pharynx, 5 sides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. Each slide contains tissue from a single human tumor. Documentation of tissues' clinical histories may be available upon request. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Biochain Institute Inc Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) VIII, 5 slides/PK
BioChain's human adult normal multiple tissue panels are designed for studying tissue specific cellular localization of genes or proteins of interest in different human normal tissues. Tissues mounted on positively charged glass slides are organized either by organs (major organ multiple tissue panels), or by integrated major systems (organ systems multiple tissue panels). Documentation of tissues' clinical histories is available upon request. Identical format total RNA Northern Blots are available, and cDNA from those tissues may also be available for PCR detection.
Biochain Institute Inc Frozen Tissue Section - Human Diabetic Diseased Tissue: Adrenal, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Montana Molecular Human Melanocortin 1 Receptor (MC1R) Big Sky BacMam, 15mL
Human Melanocortin 1 Receptor (MC1R) packaged in Big Sky BacMam for robust expression in mammalian cells. BacMam packaging provides efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in primary or iPSC-derived cells. BacMam expression is easily titrateable to suit your experimental needs.
Amsbio LLC TLR8/NF-kB Leeporter™ Luciferase Reporter-HEK293 Cell Line
TLR8/NF-kB Leeporter™ Luciferase Reporter-HEK293 Cell Line
Eurofins Discoverx PathHunter CHO-K1 GHSR1b β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0354C2A)
The PathHunter GHSR1b cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. ghrelin) based activation of GHSR1b GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GHSR1b and EA-tagged β-Arrestin-2. Activation of GHSR1b stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 GPR25 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0368C2A)
The PathHunter GPR25 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR25 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR25 and EA-tagged β-Arrestin-2. Activation of GPR25 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.