General Cell Lines and Cell Cultures
Lonza Walkersville Hu Melanocyte - Neo Nufe Kit
The Nucleofector 2b Device (or older generations I or II) works with cell type specific kits each of them dedicated to an individual primary cell This Nucleofector Kit is the optimal kit for efficient transfectiion of primary human melanocytes The respective cell type-specific Optimized Protocol or recommendation is available under Instructions or in our Lonza Bioscience knowledge database In this Optimized Protocol the best Nucleofection conditions are indicated In addition we share our experience and knowledge for treatment of this cell type
Brainxell Inc Microglia (Human iPSC-Derived, Female Line, 2M)
Microglia derived from iPSC line WC-32, female. Contents: 1 vial of ≥2x10^6 viable cryopreserved cells. Marker Expression: Confirmed expression of microglia surface markers including: CD45, CD11b, CX3CR1, P2RY12, and TMEM119. Immunocytochemistry shortly after plating microglia (shown here with a DAPI stain) reveals prominent expression of TREM2 (green) and IBA1 (red). Function: Human microglia demonstrate phagocytosis and can be stimulated to secrete a variety of cytokines. iPSC derived microglia are immunologically naive. Related diseases: Autism, Epilepsy, Multiple Sclerosis, Alzheimer's & Huntington's Disease.
Eurofins Discoverx ChemiBrite GPR120 Receptor Stable Cell Line, 2 vials. (HTS225L)
The ChemiBrite GPR120 Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. free fatty acids) based activation of FFAR4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FFAR4, a naturally Gq coupled GPCR. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of FFAR4, the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx PrecisION hGABAA α5/β3/γ2 Recombinant Stable Cell Line, 2 vials. (CYL3073)
The PrecisION hGABAA α5/β3/γ2 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying GABAA α5/β3/γ2 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses GABAA α5/β3/γ2 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Biochain Institute Inc Paraffin Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue II, 7 different tissues, 5 slides/PK
BioChain's FFPE Alzheimer's diseased brain tissue panels are designed for rapidly screening Alzheimer's disease-specific genes or proteins in brain tissues. Tissues are arrayed on positively charged glass slides. Alzheimer's diseased brain tissues on T8236444Alz are selected based on functional regions, while those on T8236445Alz are selected based on anatomical regions. T8236564Alz contains both functional and anatomical regions of Alzheimer's diseased brain tissues. Normal brain tissue panels are also available in the identical format as controls. T8236446Alz contains both Alzheimer's diseased brain tissues and normal brain tissues.
ATCC Legionella anisa; Strain WA-316-C3; genomic DNA
Genomic DNA isolated from Legionella anisa strain WA-316-C3. This bacterial strain is also available as ATCC Catalog No. 35292™.
Eurofins Discoverx ChemiScreen H1 Histamine Receptor Membrane Preparation, 1 vial. (HTS050M)
The ChemiScreen H1 Histamine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of HRH1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx PathHunter U2OS IL5RA/CSF2RB Dimerization Cell Line, 2 vials. (93-0972C3)
The PathHunter IL5RA/CSF2RB Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. IL-5) induced dimerization of IL5RA/CSF2RB receptors. This product uses EFC technology. The cell line included overexpresses CSF2RB target protein fused to PK alongside IL5RA target protein fused to EA. Upon ligand-induced dimerization of IL5RA/CSF2RB, detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/interleukins.
Bacillus spizizenii strain NRS 231 (ATCC 6633™)
Bacillus spizizenii strain NRS 231 (ATCC 6633™) is a whole-genome sequenced strain used in antimicrobial, sporicidal, and antibiotic potency testing. This strain is recommended as a test organism in USP <2021>, <61>, <71>, and <81>.
Recommended companion products:
MicroQuant™ Bacillus spizizenii, low CFU (Fisher Cat. No. NC3456053, ATCC 6633-LQ-PACK™)
Genomic DNA (Fisher Cat. No. 50-238-4902, ATCC 6633DQ™).
ATCC Influenza B virus; B/Taiwan/2/62; RNA
Genomic RNA isolated from a preparation of cell lysate and supernatant from MDCK cells (ATCC CCL-34) infected with Influenza B virus strain B/Taiwan/2/62 (ATCC VR-1735). This product was prepared using methods known to inactivate viruses. It is suitable for use in RT-PCR or other molecular based procedures. The source organism and host cells are also available through the ATCC catalog.
Biochain Institute Inc Paraffin Tissue Section - hypertension: Heart Ventricle, left, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. Each slide contains tissue from single human diseased sample. Documentation of tissues' clinical histories may be available upon request. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
ATCC RT4; Bladder Papilloma; Human (Homo sapiens)
RT4 is a cell line exhibiting epithelial morphology that was isolated from urinary bladder tissue derived from a 63-year-old, White, male patient with transitional cell papilloma. Use these cells in your immuno-oncology research.