General Cell Lines and Cell Cultures
Promocell GmbH PromoCell HDBEC Pellet from > 1 million Normal Human Dermal Blood Endothelial Cells
Cell pellet consisting of 1 million cells dissolved in 200µl RNAlater for subsequent RNA, DNA or protein analysis. Note: Cell pellets cannot be revived.Our Cell Pellets are stored in RNAlater – an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes our Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.Each Cell Pellet is made from 1 million cells and is dissolved in 200 µl RNAlater. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.
As One International Inc Green Fluorescent primary HUVEC - tube formation assay cells, 3 x 10^6 cells
Green Fluorescent primary HUVEC - tube formation assay cells, 3 x 10^6 cells
Promocell GmbH PromoCell NHEK-c 500,000 Human Epidermal Keratinocytes, single donor, adult, cryopreserved
Primary Normal Human Epidermal Keratinocytes (NHEK) are available from single or from pooled donors isolated from the epidermis of juvenile foreskin or adult skin from different locations like the face, the breasts, the abdomen, and the thighs. They are the major cell type in the epidermis, making up about 90% of the cells.Epidermal keratinocytes originate in the stratum basale and move up through the layers of the epidermis. During this movement, they undergo gradual differentiation and morphology changes until they reach the stratum corneum, where they form a layer of nucleus-free, flat, and highly keratinized squamous cells. This layer forms an effective barrier to the entry of infectious agents into the body and minimizes moisture loss.Keratinocytes are also able to produce a variety of cytokines, growth factors, interleukins and complement factors. Therefore keratinocytes are important for wound healing, inflammation, and immune response.
Promocell GmbH PromoCell HAoSMC-c 500,000 Human Aortic Smooth Muscle Cells, cryopreserved
Primary Human Aortic Smooth Muscle Cells (HAoSMC) are isolated from plaque-free regions of the human aorta and stain positive for smooth muscle α-actin. The pulsatile pressure produced by the heart causes a cyclic distention of the aorta. The smooth muscle cells in the aortic wall subsequently contract it again. Changes in the arterial wall, associated with vascular diseases such as atherosclerosis and hypertension, strongly influence this process.HAoSMC are suitable for studying the role of smooth muscle cells under normal or disease conditions in vitro.Our HAoSMC are now also available from HLA-typed and Diabetes Type I and Type II donors.
Promocell GmbH PromoCell HDMEC Pellet from > 1 million Normal Human Dermal Microvascular Endothelial Cells, pre-screened
Cell pellet consisting of 1 million cells dissolved in 200µl RNAlater for subsequent RNA, DNA or protein analysis. Note: Cell pellets cannot be revived.Our Cell Pellets are stored in RNAlater – an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes our Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.Each Cell Pellet is made from 1 million cells and is dissolved in 200 µl RNAlater. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.
Eurofins Discoverx cAMP Hunter CHO-K1 GIPR Gs Cell Line, 2 vials. (95-0146C2)
The cAMP Hunter GIPR Gs cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. GIP) based activation of GIPR GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GIPR, a naturally Gs coupled GPCR. Upon activation of GIPR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx Ready-to-Assay CXCR1 Chemokine Receptor Frozen Cells, 2 Vials of Cells. (HTS001RTA)
The Ready-to-Assay CXCR1 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. CXCL8/IL8) based activation of CXCR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CXCR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CXCR1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Miltenyi Biotec, Inc. Tumor-Asso. Fibroblast Isolation Kit, m
The Tumor-Associated Fibroblast Isolation Kit, mouse enables the isolation of tissue-derived, tumor-associated fibroblasts from dissociated mouse tumors
STEMCELL Technologies Human Bone Marrow CD34+ Cells, Frozen, Size: 5 x 10^5 cells
Start experiments confidently with ready-to-use, ethically sourced, CD34+ stem and progenitor cells from adult human bone marrow. With personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications, we help you get the cells you need. Isolated immunomagnetically using positive selection and cryopreserved in serum-free medium, cells are collected using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation to meet institutional requirements and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Heparin is added during collection as an anticoagulant. Contact techsupport@stemcell.com for donor specifications (BMI category, smoking status, ethnicity, etc.). Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C. Certain products are only available in select territories.
Alomone Labs, LTD Alomone Labs Polyclonal Antibody, Rabbit, Human, Mouse, Rat, Unconjugated
This polyclonal antibody targets CRLR (Calcitonin Receptor-Like Receptor), a receptor for calcitonin gene-related peptide type 1. Activity is mediated by G proteins, activating adenylyl cyclase. CRLR is expressed in brain, lung, blood vessel, liver, and intestinal tract.
- Host/Isotype: Rabbit IgG
- Reactivity: Human, mouse, and rat
- Immunogen: Peptide from human Calcitonin Receptor-Like Receptor (extracellular, N-terminus).
- Conjugate: Unconjugated
- Form: Lyophilized
- Concentration: 0.8 mg/mL
- Amount: 160 μg
- Purification: Antigen affinity chromatography
- Storage buffer: PBS, pH 7.4, with 1% BSA and 0.05% sodium azide
- Applications: WB (1:200), IHC (F) (1:200), Flow (2.5 μg).
- Storage conditions: -20°C, avoid freeze/thaw cycles.
- Shipping conditions: Ambient (domestic); wet ice (international).
Electron Microscopy Sciences Quadruple Geological Thin Section Holder, Hitachi M4
This thin section holder made from aluminum with copper clips and brass screws. The unit measures 107x58x8 mm and it holds four standard geological thin sections or slides of 47 x 27 mm. The holder has convenient tweezer inserts which allow for easy loading. Clips are provided for secure holding.
Type: Hitachi M4
Lonza Walkersville PYROGENT Plus Gel Clot LAL Assay (with endotoxin)
The PYROGENT Plus Gel Clot LAL Assay combines PYROGENT LAL with a matched control standard endotoxin together in one box kit. Standard kit sizes include 80 tests or 64 tests. The 80 and 64-test kits require depyrogentated 10 x 75 mm glass reaction tubes to run the assay. Benefits:Sensitivity of 0.06 EU/ml Easy-to-read qualitative results No need to purchase CSE separatelyApplications:Simple LAL test not requiring sophisticated instrumentation and softwareContent:4 x 16 tests/vial lysate
Eurofins Discoverx PathHunter CHO-K1 NMBR β-Arrestin Cell Line, 2 vials. (93-0925C2)
The PathHunter NMBR cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. neuromedin B) based activation of NMBR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NMBR and EA-tagged β-Arrestin-2. Activation of NMBR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 GPR139 β-Arrestin Cell Line, 2 vials. (93-0954C2)
The PathHunter GPR139 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. TC-O 9311) based activation of GPR139 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR139 and EA-tagged β-Arrestin-2. Activation of GPR139 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.