General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter U2OS TYRO3 Functional Assay, 2 vials. (93-1110C3)
The PathHunter U2OS TYRO3 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TYRO3 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TYRO3 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx ChemiScreen NK1 Tackykinin Receptor Stable Cell Line, 2 vials. (HTS080C)
The ChemiScreen NK1 Tachykinin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. tachykinins) based activation of TACR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses TACR1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of TACR1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
ATCC Rhodospirillum rubrum
Rhodospirillum rubrum strain NCIB 8255 is a whole-genome sequenced type strain. This phototroph is tolerant to oxygen, so strictly anoxic conditions are not required when the vial is thawed.
Promocell GmbH PromoCell NHEM.f Pellet from > 1 million Normal Human Epidermal melanocytes, derived from foreskin
Cell pellet consisting of 1 million cells dissolved in 200µl RNAlater for subsequent RNA, DNA or protein analysis. Note: Cell pellets cannot be revived.Our Cell Pellets are stored in RNAlater – an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes our Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.Each Cell Pellet is made from 1 million cells and is dissolved in 200 µl RNAlater. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.
STEMCELL Technologies Human Whole Peripheral Blood Collected with ACDA, Fresh, Size: 60 mL
Normal whole peripheral blood (PB) is obtained from healthy donors using Institutional Review Board (IRB)-approved consent forms and protocols. Acid citrate dextrose, solution A (ACDA), Sodium Heparin (Na-Hep), Ethylenediaminetetraacetic acid (EDTA), or Citrate-phosphate-double-dextrose (CP2D) is the anticoagulant.Certain products are only available in select territories. Please contact your local Sales representative or Product & Scientific Support at techsupport@stemcell.com for further information and to inquire about donor selection or information including BMI category, smoking status, ethnicity, etc.Browse our Frequently Asked Questions (FAQs) (https://www.stemcell.com/primary-cells-faq) on Primary Cells.
Eurofins Discoverx Ready-to-Assay NTR1 Neurotensin Receptor Frozen Cells, 2 Vials of Cells. (HTS034RTA)
The Ready-to-Assay NTR1 Neurotensin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. neurotensin) based activation of NTSR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses NTSR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of NTSR1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
iQ Biosciences Purified Human CD4 T Cells, 10M cells/vial
High-quality, high-viability (> 90%) human CD4 T cells purified from PBMCs of healthy blood donors sourced from a world-renowned blood center. Highly enriched (typically > 90%) post-selection purity achieved via magnetic isolation. All orders come with an iQ Certificate of Analysis.
ATCC Sendai virus; Strain: Sendai/52
Sendai virus strain Sendai/52 is propagated in SPF chicken embryonated eggs. This strain was isolated from the lung of a patient with a fatal case of newborn pneumonitis in Japan in 1952 and was deposited by the Centers for Disease Control and Prevention. The virus has applications in respiratory disease research.
Eurofins Discoverx PathHunter CHO-K1 P2RY12 β-Arrestin Cell Line, 2 vials. (93-0262C2)
The PathHunter P2RY12 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. ATP) based activation of P2RY12 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY12 and EA-tagged β-Arrestin-2. Activation of P2RY12 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biochain Institute Inc Frozen Tissue Section - Human Adult Normal: Brain: Optic Nerve, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Eurofins Discoverx PathHunter CHO-K1 GPR35 β-Arrestin Cell Line, 2 vials. (93-0942C2)
The PathHunter GPR35 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. zaprinast) based activation of GPR35 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR35 and EA-tagged β-Arrestin-2. Activation of GPR35 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC pRS426 phagemid; in E. coli
This is a YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. It can be used to produce ssDNA and it contains promoters for in vitro RNA synthesis and priming sites useful for sequencing. It also contains the REP3 and FRT sequences necessary for high copy propagation in yeast. In S. cerevisiae, the copy number is about 20 per haploid cell. In non-selective growth, plasmids are lost through mitotic segregation at rates in the range of 4.4 +/- 1.4% of progeny per doubling.
Cayman Chemical al-trns RetInal 1g
A natural retinoid that associates with CRBP-I and CRBP-II (Kds = 50 and 90 nM, respectively), which are involved in the intracellular transport of retinol; an intermediate of the phototransduction pathway of photoreceptors; can form toxic conjugates with proteins and lipids, leading to degeneration of the retina