General Cell Lines and Cell Cultures
Lonza Walkersville MycoAlert PLUS Mycoplasma Detection Kit (10 Tests)
Lonza’s MycoAlert Mycoplasma Detection Assays are selective biochemical tests to detect Mycoplasma contamination in cell cultures. They exploit the activity of mycoplasmal enzymes which are found in the vast majority of about 200 mycoplasma species, but are not present in eukaryotic cells.Benefits:Easy assay: Simply add two reagents to the culture supernatant and perform two luminescence readingsResults in < 20 minEasy interpretation of resultsApplications:Detects all common mollicute contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma)Suited for tube luminometers, plate luminometers and multifunctional readersContent:1 x 1.2 mL MycoAlert PLUS Reagent (lyophilized)1 x 1.2 mL MycoAlert PLUS Substrate (lyophilized)1 x 3 mL MycoAlert PLUS Assay Buffer
Promocell GmbH PromoCell NHEM.f Pellet from > 1 million Normal Human Epidermal melanocytes, derived from foreskin
Cell pellet consisting of 1 million cells dissolved in 200µl RNAlater for subsequent RNA, DNA or protein analysis. Note: Cell pellets cannot be revived.Our Cell Pellets are stored in RNAlater – an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes our Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.Each Cell Pellet is made from 1 million cells and is dissolved in 200 µl RNAlater. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.
Promocell GmbH PromoCell hMSC-BM Pellet from > 1 million Normal Human Mesenchymal Stem Cells, derived from Bone Marrow
Cell pellet consisting of 1 million cells dissolved in 200µl RNAlater for subsequent RNA, DNA or protein analysis. Note: Cell pellets cannot be revived.Our Cell Pellets are stored in RNAlater – an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes our Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.Each Cell Pellet is made from 1 million cells and is dissolved in 200 µl RNAlater. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.
Lifeline Cell Technology CELLS LUNG SM HU LD LIFELN VL
Lifeline's normal Human Lung Smooth Muscle Cells, when grown in VascuLife SMC Medium, provide an ideal culture model for the study of angiogenesis, atherosclerosis, diabetes or vascular/pulmonary biology.
Lifeline Cell Technology CELLS HUVEC LIFELINE VL
Normal Human Umbilical Vein Endothelial Cells (HUVEC), cryopreserved as primary cells. Quality tested in VascuLife 2% serum medium to ensure optimal reduced-serum growth over a period of at least 15 population doublings.
Promocell GmbH PromoCell HUAEC-c 500,000 Human Umbilical Artery Endothelial Cells, cryopreserved
Primary Human Umbilical Artery Endothelial Cells (HUAEC) are isolated from the arteries of the umbilical cord and are often used for in vitro studies on atherosclerosis but also for endothelial cell-dependent lymphocyte activation and chemoattractant activity experiments.Available formats:* Cryopreserved: Cryogenic vial containing 500.000 viable cells.* Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).* Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
Promocell GmbH PromoCell HUF-c 500,000 Human Uterine Fibroblasts, cryopreserved
Primary Human Uterine Fibroblasts (HUF) are isolated from the uterine wall. During pregnancy, the uterine wall undergoes deep but reversible structural and biochemical changes. The uterine wall is the target of hormones and growth factors that act simultaneously and lead to extensive modifications of the extracellular matrix. This and other morphological and biochemical effects can be studied in vitro using HUF. Available formats:* Cryopreserved: Cryogenic vial containing 500.000 viable cells.
Promocell GmbH PromoCell NHEM.f-c 500,000 Human Epidermal Melanocytes, derived from foreskin, cryopreserved
Primary Normal Human Epidermal Melanocytes (NHEM) are isolated from the epidermis of juvenile foreskin.Melanocytes are located in the stratum basale, but branch out between the keratinocytes in suprabasal layers. About 5-10 % of the cells in the epidermis are melanocytes. The main purpose of melanocytes is the production of melanin, the protein responsible for the pigmentation of the skin, eyes, and hair. Melanin protects the cells in the skin and in deeper layers from the hazardous effects of UV radiation. It is produced and stored in melanosomes, which are located close to the melanocyte membrane, but it can also be transferred to neighboring keratinocytes.PromoCell Normal Human Epidermal Melanocytes are isolated using the PMA (Phorbol Myristate Acetate)-containing Melanocyte Growth Medium. Since PMA is a tumor promoting mitogen, it can interfere with experimental approaches. Therefore, we recommend using cells isolated in Melanocyte Growth Medium M3.
Promocell GmbH PromoCell NHEK-c 500,000 Human Epidermal Keratinocytes, single donor, adult, cryopreserved
Primary Normal Human Epidermal Keratinocytes (NHEK) are available from single or from pooled donors isolated from the epidermis of juvenile foreskin or adult skin from different locations like the face, the breasts, the abdomen, and the thighs. They are the major cell type in the epidermis, making up about 90% of the cells.Epidermal keratinocytes originate in the stratum basale and move up through the layers of the epidermis. During this movement, they undergo gradual differentiation and morphology changes until they reach the stratum corneum, where they form a layer of nucleus-free, flat, and highly keratinized squamous cells. This layer forms an effective barrier to the entry of infectious agents into the body and minimizes moisture loss.Keratinocytes are also able to produce a variety of cytokines, growth factors, interleukins and complement factors. Therefore keratinocytes are important for wound healing, inflammation, and immune response.
Promocell GmbH PromoCell HDMEC Pellet from > 1 million Normal Human Dermal Microvascular Endothelial Cells, pre-screened
Cell pellet consisting of 1 million cells dissolved in 200µl RNAlater for subsequent RNA, DNA or protein analysis. Note: Cell pellets cannot be revived.Our Cell Pellets are stored in RNAlater – an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes our Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.Each Cell Pellet is made from 1 million cells and is dissolved in 200 µl RNAlater. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.
Promocell GmbH PromoCell hMoCD14+-PB Pellet from > 1 million Normal Human CD14+ Monocytes, derived from Peripheral Blood, single donor
Cell pellet consisting of 1 million cells dissolved in 200µl RNAlater for subsequent RNA, DNA or protein analysis. Note: Cell pellets cannot be revived.Our Cell Pellets are stored in RNAlater – an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes our Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.Each Cell Pellet is made from 1 million cells and is dissolved in 200 µl RNAlater. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.
Promocell GmbH PromoCell HBSMC Pellet from > 1 million Normal Human Bronchial Smooth Muscle Cells
Cell pellet consisting of 1 million cells dissolved in 200µl RNAlater for subsequent RNA, DNA or protein analysis. Note: Cell pellets cannot be revived.Our Cell Pellets are stored in RNAlater – an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes our Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.Each Cell Pellet is made from 1 million cells and is dissolved in 200 µl RNAlater. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.
Promocell GmbH PromoCell HDLEC-c 500,000 Human Dermal Lymphatic Endothelial Cells, cryopreserved
Primary Human Dermal Lymphatic Endothelial Cells (HDLEC) are a subpopulation of the Human Dermal Endothelial Cells. They are isolated from the dermis of juvenile foreskin and adult skin (different locations) from a single donor.The cells are routinely analyzed by flow cytometric analysis and immunofluorescent staining:> 95% of the cells are CD31 positive and podoplanin positive.Lymphatic vessels transport excess fluids from tissues to the circulatory system and are a major component of the immune system. The transported fluid is nearly cell free. The vessels are highly permeable, because they lack a continuous basal membrane. Lymphatic endothelial cells are involved in pathological alterations of the lymphatic system. During tumor lymphangiogenesis, the lymphatic endothelial cells build new vessels that infiltrate tumors, attract tumor cells, and induce tumor cell metastasis.
Promocell GmbH PromoCell NHEK.f-c 500,000 Human Epidermal Keratinocytes, derived from foreskin, pooled, cryopreserved
Primary Normal Human Epidermal Keratinocytes (NHEK) are available from single or from pooled donors isolated from the epidermis of juvenile foreskin or adult skin from different locations like the face, the breasts, the abdomen, and the thighs. They are the major cell type in the epidermis, making up about 90% of the cells.Epidermal keratinocytes originate in the stratum basale and move up through the layers of the epidermis. During this movement, they undergo gradual differentiation and morphology changes until they reach the stratum corneum, where they form a layer of nucleus-free, flat, and highly keratinized squamous cells. This layer forms an effective barrier to the entry of infectious agents into the body and minimizes moisture loss.Keratinocytes are also able to produce a variety of cytokines, growth factors, interleukins and complement factors. Therefore keratinocytes are important for wound healing, inflammation, and immune response.