General Cell Lines and Cell Cultures
Biochain Institute Inc Frozen Tissue Section - Human Adult Normal: Heart: Ventricle (right), 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Biolegend PE anti-mouse CD24 Recombinant
PE anti-mouse CD24 Recombinant [QA20A91]; Isotype: Mouse IgG1, κ; Reactivity: Mouse; Apps: FC; Size: 25 ug
Eurofins Discoverx PathHunter CHO-K1 rGPR35 β-Arrestin Cell Line, 2 vials. (93-0887C2)
The PathHunter rGPR35 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. zaprinast) based activation of rGPR35 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rGPR35 and EA-tagged β-Arrestin-2. Activation of rGPR35 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biolegend TotalSeqTM-C0242 anti-human CD
TotalSeq™-C0242 anti-human CD192 (CCR2) [K036C2]; Isotype: Mouse IgG2a, κ; Reactivity: Human; Apps: PG; Size: 10 μg
Biochain Institute Inc Breast Tumor Tissue Array - Duplicated 70 cases covering all the common types of breast cancer and 5 cases of normal and, 5 slides/PK
BioChain's tissue arrays or tissue microarrays (TMAs) are a useful tool in biomarker research and drug target validation. It consists of paraffin blocks that are assembled in array fashion to allow multiplex histological analysis. BioChain's TMAs provide a valuable tool for analysis of protein, RNA, or DNA molecules in oncology, both the malignant and metastatic markers. TMAs can also been constructed with tissues from other diseases and species.
Eurofins Discoverx PathHunter CHRM2 Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0656C3)
The PathHunter CHRM2 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of CHRM2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CHRM2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CHRM2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx ChemiScreen CCR9 Chemokine Receptor Stable Cell Line, 2 vials. (HTS036C)
The ChemiScreen CCR9 Chemokine Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CCL25/TECK) based activation of CCR9 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CCR9 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CCR9, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
ATCC M1; Myeloid Leukemia; Mouse (Mus musculus)
M1 is a myeloblast cell isolated from a mouse with myeloid leukemia and can be used in immune system disorder research. The cell can be induced to become macrophage-like by a variety of stimulating agents including dexamethasone, LPS, and WEHI-3 conditioned medium.
Eurofins Discoverx PathHunter HEK 293 TPOR/TPOR Dimerization Cell Line, 2 vials. (93-1108C1)
The PathHunter TPOR/TPOR Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. TPO) induced dimerization of TPOR/TPOR receptors. This product uses EFC technology. The cell line included overexpresses TPOR target protein fused to PK alongside TPOR target protein fused to EA. Upon ligand-induced dimerization of TPOR/TPOR, detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/interleukins.
Promocell GmbH PromoCell HPF-c 500,000 Human Pulmonary Fibroblasts, cryopreserved
Primary Human Pulmonary Fibroblasts (HPF) are isolated from human lung tissue. They are often used for studying lung tissue repair, tissue remodeling after injury, or the response after pulmonary inflammation. Fibroblast disorders like pulmonary fibrosis or hypoxia-induced pulmonary hypertension can also be investigated with cultured HPF.
Eurofins Discoverx ChemiScreen GIP Glucagon Receptor Stable Cell Line, 2 vials. (HTS134C)
The ChemiScreen GIP Glucagon Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. GIP) based activation of GIPR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GIPR alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of GIPR, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen CCK2 Cholecystokinin Receptor Membrane Preparation, 1 vial. (HTS083M)
The ChemiScreen CCK2 Cholecystokinin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CCKBR surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
ATCC NCI-H2081; Lung Carcinoma; Human (Homo sapiens)
NCI-H2081 [H2081] is a cell line that was isolated from the lungs of a 59-year-old, White, female patient with stage E small cell lung cancer: carcinoma. This product has applications in cancer research.
ATCC Mycobacterium tuberculosis; Quantitative DNA
Quantitative Genomic DNA from Mycobacterium tuberculosis strain H37Ra (ATCC 25177) can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load and for other molecular biology applications.