General Cell Lines and Cell Cultures
Cell Applications Inc Proliferating Synoviocytes (HFLS), Adult
Proliferating Synoviocytes (HFLS), Adult
ATCC Microsporum canis
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
Cell Applications Inc Proliferating Canine Skeletal Muscle Cells (CnSkMC)
Proliferating Canine Skeletal Muscle Cells (CnSkMC)
Eurofins Discoverx ChemiScreen CCR1 Chemokine Receptor Membrane Preparation, 1 vial. (HTS005M)
The ChemiScreen CCR1 Chemokine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CCR1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx Ready-to-Assay CCR4 Chemokine Receptor Frozen Cells, 2 Vials of Cells. (HTS009RTA)
The Ready-to-Assay CCR4 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. MIP, RANTES) based activation of CCR4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CCR4 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CCR4, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ACROBiosystems HEK293/Human PTK7 Stable Cell Line
PTK7, highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
Eurofins Discoverx ChemiScreen Glucagon Receptor Stable Cell Line, 2 vials. (HTS112C)
The ChemiScreen Glucagon Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. glucagon) based activation of GCGR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GCGR alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of GCGR, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
ATCC Salmonella enterica; subsp. enterica; serovar Paratyphi A
Salmonella enterica subsp. enterica serovar Paratyphi A strain CDC has applications in enteric disease research and zoonotic disease research. This bacterium is serotype 2,12:a:-.
ATCC Quantitative Synthetic; Bourbon virus RNA
Quantitative Synthetic Bourbon virus RNA can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load. Preparation includes fragments from the PB1 and NP regions.
Eurofins Discoverx InCELL Hunter Jurkat RIPK3 Kinase Binding Cell Line, 2 vials. (96-0077C19)
The InCELL Hunter Jurkat RIPK3 Kinase Binding Cell Line is a stable, engineered cell line for use in studying small molecule drug candidates that engage with kinases. This cell line enables assessment of compound-engagement-based stabilization of RIPK3 via detection of RIPK3 levels. This product uses EFC technology. The cell line included overexpresses RIPK3 target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is denatured by the heat treatment and no EFC signal is produced. When compound engages and heat-stabilizes the target, EA contained in the detection reagent produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PathHunter C5L2 Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0736C3)
The PathHunter C5L2 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of C5L2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged C5L2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting C5L2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ABclonal Technology GNRHR Rabbit pAb
This gene encodes the receptor for type 1 gonadotropin-releasing hormone. This receptor is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Following binding of gonadotropin-releasing hormone, the receptor associates with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Activation of the receptor ultimately causes the release of gonadotropic luteinizing hormone (LH) and follicle stimulating hormone (FSH). Defects in this gene are a cause of hypogonadotropic hypogonadism (HH). Alternative splicing results in multiple transcript variants encoding different isoforms. More than 18 transcription initiation sites in the 5 region and multiple polyA signals in the 3 region have been identified for this gene.
Eurofins Discoverx PathHunter U2OS NPSR1 β-Arrestin Cell Line, 2 vials. (93-0822C3)
The PathHunter NPSR1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. neuropeptide S) based activation of NPSR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NPSR1 and EA-tagged β-Arrestin-2. Activation of NPSR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.