General Cell Lines and Cell Cultures
Lonza Walkersville Human Dermal Fibroblast Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human fibroblasts, e.g. NHDF-neo.The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human Dermal Fibroblasts0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Medchemexpress LLC Icos protein, rat (HEK293, Fc) | >95.0% | 5 UG
Recombinant rat ICOS (inducible T-cell co-stimulator) protein expressed in HEK293 cells and fused to a C-terminal human Fc tag. Supplied lyophilized from PBS (pH 7.4), the glycosylated Fc-fusion spans residues E21-L142 with an estimated molecular weight of ~43-50 kDa. Designed for research into T cell co-stimulation and immune signaling.
- Purity >95% by reducing SDS-PAGE.
- Contains C-terminal human Fc tag for detection and capture.
- Supplied lyophilized for extended shelf life and transport.
- Expressed in HEK293 cells to provide mammalian glycosylation.
- Amino acid range E21-L142 corresponds to extracellular domain.
- Recommended storage at -20°C; reconstituted material stable at 4°C for 1 week.
- Available in research-scale sizes including a 5 μg package.
Lonza Walkersville RCGM Rat Cardiomyocyte Growth
RCGM Rat Cardiomyocyte Growth Medium is specifically formulated for the growth and survival of rat neonatal cardiac myocytes in culture Lonza offers customers a fully validated and optimized cardiomyocyte medium and growth factors to complement our Clonetics Rat Cardiomyocytes R-CM-561 as a complete system to study cardiac biology/toxicology Basal medium and SingleQuots Kit growth factors are available separately Media products are specially designed to support the growth of human and animal primary cells Media systems have been specifically developed and optimized for various cell systems Media are supplied in several formats Basal Medium - contains no growth factors cytokines or supplements SingleQuots - growth factors cytokines and supplements BulletKits - basal media and SingleQuots Kit packaged together
Lonza Walkersville Hu BM CD34 Prog Cells 500k
Bone marrow CD34 cells contain hematopoietic stem and progenitor cells and are known to differentiate into all the various blood cell types Lonza bone marrow CD34 cells are isolated from bone marrow mononuclear cells (MNC) by positive immunomagnetic separation and are guaranteed to have a purity of 90% They are guaranteed to contain either 100 000 300 000 500 000 1 million or 2 million viable cells per ampoule depending on the product ordered HIV-1 hepatitis C and hepatitis B are not detected for all donors and/or cell lots A Certificate of Analysis is provided for each cell lot purchased Fresh bone marrow CD34 cells are offered in various packaging sizes and require a minimum order quantity of two vials per donor Please contact Customer Service for information on sizes and pricing for fresh cells
Genscript Corporation CHO-K1/G15/AMY2 Stable Cell L
Receptor activity-modifying proteins (RAMPs) are a class of protein which interact with and modulate the activities of several Class B G Protein-Coupled Receptors including the receptors for secretin calcitonin (CT) glucagon and vasoactive intestinal peptide (VIP) There are three distinct types of RAMPs designated RAMP1 RAMP2 and RAMP3 each encoded by a separate gene Currently the function of RAMPs is divided into 2 class activities Association of RAMPs with either the CT or CALCR proteins forms 6 different receptors from the calcitonin receptor family When associated with the Calcitonin receptor (CTR) or Calcitonin receptor-like (CALCRL) RAMPs can change the selectivity of the receptor for a specific hormone In the cases of the other receptors mentioned however there is no evidence that they can do this but instead function to regulate trafficking of receptors from the ER / golgi to the membrane GenScript s cloned human AMY2 (RAMP2 CALCR) expressing cell line is gen
Amsbio LLC Normal Human Normal Human Intr
Normal Human Normal Human Intrahepatic Biliary Epithelial Cells (Cholagioctyes); passage 2
Genscript Corporation CHO-K1/B7-H4 Stable Cell Line
B7-H4 is a transmembrane protein that binds an unknown receptor on activated T cells resulting in inhibition of T-cell effector function via cell cycle arrest decreased proliferation and reduced IL-2 production B7-H4 is up-regulated on the surface of cancer cells and immunosuppressive tumor-associated macrophages (TAMs) in a variety of human cancers
Angio Proteomie Human Alveolar Epithelial Cells, Frozen Vial, > 5e5 cells/vial, Passage 2, Population Doubling Levels > 15, Research Use Only, -80C short-term storage, Liquid nitrogen long-term storage
Human Alveolar Epithelial Cells, Frozen Vial, > 5e5 cells/vial, Passage 2, Population Doubling Levels > 15, Research Use Only, -80C short-term storage, Liquid nitrogen long-term storage
Discovery Life Sciences Rat (Sprague-Dawley) Pooled Liver S9 | Male | 1.0 mL
This S9 product is prepared from sexually mature male Sprague-Dawley rat livers and characterized for key cytochrome P450 and sulfotransferase enzyme activities.