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A butyrate ester of carnitine; blocks carnitine uptake by the carnitine transporter and glycine transport by the amino acid transporter in HRPE cells (IC50s = 1.5 µM and 4.6 mM, respectively)
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An adenosine receptor agonist; selectively binds to adenosine A1 receptors in rat cortical membranes (IC50 = 2.3 nM) over A2 receptors in rat striatal membranes (IC50 = 870 nM); decreases heart rate and increases coronary flow in a perfused working rat heart model ex vivo (EC25s = 5 and 860 nM, respectively); decreases heart rate and blood pressure in normotensive rats in vivo (EC25s = 2.4 and 4.2 μg/kg, respectively); induces sleep in rats via basal forebrain infusion at 100 μM; decreases locomotor activity in mice (ED50 = 60 μg/kg, i.p.)
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A Bim-derived peptide; binds to Mcl-1, Bcl-xL, Bcl-2, and Bcl-W (IC50s = 18, 10, 7, and 18 nM, respectively); induces cytochrome c release from mitochondria derived from bcl-x-/- MEFs
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A fungal metabolite and benzoic acid derivative with antioxidant and neuroprotective activities; scavenges DPPH radicals (IC50 = 5 mM); prevents PAF-induced PARP cleavage in PC12-AC cells and PAF-induced cytotoxicity in Pafr-/-mouse cerebellar granule cells at 1 μg/ml
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Free or conjugated antibodies are often used in immunoassays, either in the system specific detector antibody plus streptavidin-HRP or in the system analyte-specific antibody plus species-specific antibody-HRP-conjugate. Both systems allow a high flexibility at lower costs. BioStab Antibody Stabilizer dramatically extends the life of your valuable antibodies, allowing much more consistent results over extended periods of time compared to daily prepared dilutions. BioStab Antibody Stabilizer can be used in ELISA, Western Blotting, Immunohistochemistry and any other applications which require marker or biotinylated antibodies. It is supplied as a ready-to-use solution for dilution of antibodies. This stabilizer contains a minimum amount of a surfactant. The stabilizer is free of any protein to minimize potential unspecific interactions. The method below is strongly recommended for standard assay procedures and reduces loss of valuable conjugates and assay-to-assay variations.
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A prodrug form of trandolaprilat; inhibits angiotensin I-induced pressor responses in rats and dogs (ID50s = 13.1 and 21.1 μg/kg, respectively); potentiates the bradykinin-induced depressor response in rats (ED50 = 5.5 μg/kg); decreases systolic and diastolic arterial pressure in dogs at 0.1, 0.3, and 1 mg/kg; decreases MAP and LVEDP in a rat model of left coronary artery occlusion-induced heart failure at 3 mg/kg; increases survival in stroke-prone spontaneously hypertensive rats fed a high-salt diet
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A 5-HT4 agonist (Ki = 69.9 nM); selective for 5-HT4 over 5-HT1, 5-HT2, dopamine D2, and α1- and α2-adrenergic receptors at 1 mM but also acts as a partial antagonist at 5-HT3 (Ki = 1,189 nM); induces relaxation of precontracted rat esophageal thoracic muscularis mucosa preparations and enhances electrically evoked contractions in isolated guinea pig ileum (EC50s = 208.4 and 73.2 nM, respectively); increases antral and duodenal, but not ileal or colonic, motility in conscious dogs at 0.5 and 1 mg/kg; increases gastric emptying in rats from 0.03-30 mg/kg
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A thiazide diuretic; inhibitor of carbonic anhydrase (CA); selective for CAII, VB, VII, IX, XII, and XIV (Kis = 305-235 nM) over CAI, IV, and VI (Kis = 2,840-8,250 nM) and CAIII, VA, and XIII (Kis = >10 µM)
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A colorimetric β-glucuronidase substrate; upon enzymatic cleavage, p-nitrophenol is released, which can be quantified by colorimetric detection at 405 nm as a measure of β-glucuronidase activity
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Absorb Incubator Contaminants This disposable, activated charcoal cartridge efficiently absorbs low energy 35S radioactive components during metabolic labeling. The containment problem exists in incubators and is caused by 35S-labeled amino acids, such as methionine and cysteine which are used to label proteins. The charcoal is supplied in a 100 mm diameter plastic petri dish with a highly porous membrane sealed to the top edge.
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CellProSingle ServeFBS is an aliquot-free solution for serum use minimizing contamination and saving time The Single ServeFBS 50 mL bottle is designed to eliminate a tedious lab task and significantly improve your cell culture results By choosing Single ServeFBS a typical customer using 20 L of FBS per year would gain almost 7 hours back and generate 7 lb less plastic waste This is a significant benefit both to lab productivity and the environment CellProU S Source Fetal Bovine Serum is collected entirely from USDA licensed abattoirs and processed within the United States Our serum is maintained at or below 40F from collection through sterile bottling to ensure superior performance Processed serum is thawed and then heated in a water bath at 56C for 30 minutes The serum is swirled every 10 minutes to ensure proper integration of concentrated protein The heat inactivates the complement in the serum Complement is defined as a series of 20 proteins produced by the liver which am
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