The System is our All-In-One kit (without serum) optimized to elevate your experiments by providing everything needed to plate, culture, passage, and freeze cells in a convenient and consistent manner. Medium requires addition of 5-20% serum (not included) based on user's experiment. This kit includes 2x500mL Medium without serum (4Z3-500), 2x5mL CultureBoost animal-derived growth supplement to complete medium, 1x100mL Attachment Factor to enhance cell attachment, 1x50mL Cell Freezing Medium, and 1 set 100mL Passage Reagent Group (PRG) for cell release. PRG contains 3 parts: 1x100mL PRG-1 (EDTA-dPBS Solution), 1x100mL PRG-2 (Trypsin/EDTA-dPBS Solution) & 1x100mL PRG-3 (Trypsin Inhibitor-dPBS Solution). Large quantities? Contact Fisher Scientific sales representative to discuss your bulk order needs. Tight deadline? Available for next day delivery after the purchase order is received. Maximize your results! Try our Primary Human Cells which are ideal for use with our media and reagents.