Secondary Antibodies
Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies
(44,180)
Mouse Secondary Antibodies
(14,490)
Rabbit Secondary Antibodies
(6,301)
Donkey Secondary Antibodies
(4,319)
Rat Secondary Antibodies
(1,234)
Sheep Secondary Antibodies
(701)
Chicken Secondary Antibodies
(403)
Bovine Secondary Antibodies
(257)
Pig Secondary Antibodies
(76)
Horse Secondary Antibodies
(74)
Alpaca Secondary Antibodies
(62)
Hamster Secondary Antibodies
(57)
Biosimilar Secondary Antibodies
(9)
Human Secondary Antibodies
(7)
Duck Secondary Antibodies
(3)
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Goat anti-Human IgA/IgG/IgM (H+L) Secondary Antibody, Alkaline Phosphatase, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Human |
| Host Species | Goat |
| Conjugate | Alkaline Phosphatase (AP) |
| Applications | Dot Blot,ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Concentration | 1 mg/mL |
| Antigen | IgA/IgG/IgM (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:500 - 1:3000, ELISA 1:2000 - 1:10000, Immunohistochemistry 1:200 - 1:1000, Dot Blot |
| Classification | Polyclonal |
| Immunogen | Human IgG, IgA and IgM whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | This reagent is suitable for the detection of all human immunoglobulin isotypes, subclasses and chain combinations. |
Sheep anti-Rabbit IgG (H+L) Secondary Antibody, Alkaline Phosphatase (Pre-adsorbed), Novus Biologicals™
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Sheep Polyclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Rabbit |
| Host Species | Sheep |
| Conjugate | Alkaline Phosphatase (AP) |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | 1 mg/mL |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:500 - 1:2500, ELISA 1:2000 - 1:10000, Immunohistochemistry 1:200 - 1:1000 |
| Classification | Polyclonal |
| Immunogen | Rabbit IgG whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | This antibody was pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat, and Sheep Serum Proteins. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat and Sheep Serum Proteins. |
Goat anti-Mouse IgG/IgM (H+L) Secondary Antibody, Alkaline Phosphatase (Pre-adsorbed), Novus Biologicals™
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Mouse |
| Host Species | Goat |
| Conjugate | Alkaline Phosphatase (AP) |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Concentration | 1 mg/mL |
| Antigen | IgG/IgM (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:500 - 1:2500, ELISA 1:2000 - 1:10000, Immunohistochemistry 1:200 - 1:1000 |
| Classification | Polyclonal |
| Immunogen | Mouse IgG and IgM whole molecules |
| Primary or Secondary | Secondary |
| Test Specificity | No reaction was observed against bovine, horse or human serum proteins. |
Goat anti-Mouse IgA/IgG/IgM (H+L) Secondary Antibody, Alkaline Phosphatase (Pre-adsorbed), Novus Biologicals™
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Mouse |
| Host Species | Goat |
| Conjugate | Alkaline Phosphatase (AP) |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Concentration | 1 mg/mL |
| Antigen | IgA/IgG/IgM (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:500 - 1:2500, ELISA 1:2000 - 1:10000, Immunohistochemistry 1:200 - 1:1000 |
| Classification | Polyclonal |
| Immunogen | Mouse IgG IgA and IgM whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | No reaction was observed against bovine, horse or human serum proteins. This reagent is suitable for the detection of all Mouse immunoglobulin isotypes, subclasses and chain combinations. |
Goat anti-Guinea Pig IgG F(ab')2 Secondary Antibody, Alkaline Phosphatase, Novus Biologicals™
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Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Guinea Pig |
| Host Species | Goat |
| Conjugate | Alkaline Phosphatase (AP) |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Concentration | 0.5 mg/mL |
| Antigen | IgG F(ab')2 |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:500 - 1:2500, ELISA 1:2000 - 1:15000, Immunohistochemistry 1:200 - 1:1000 |
| Gene Alias | immunoglobulin heavy constant gamma 1 (G1m marker) |
| Classification | Polyclonal |
| Immunogen | Guinea Pig IgG F(ab')2 fragment |
| Primary or Secondary | Secondary |
| Test Specificity | No reaction was observed against Guinea Pig IgG F(c). |
Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alkaline Phosphatase (Pre-adsorbed), Novus Biologicals™
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Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Rabbit |
| Host Species | Goat |
| Conjugate | Alkaline Phosphatase (AP) |
| Applications | Dot Blot,ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | 1 mg/mL |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:500 - 1:2500, ELISA 1:2000 - 1:10000, Immunohistochemistry 1:200 - 1:1000, Dot Blot |
| Classification | Polyclonal |
| Immunogen | Rabbit IgG whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | This antibody was pre-adsorbed against Human Serum Proteins. No reaction was observed against Human Serum Proteins. |
Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed), Novus Biologicals™
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Goat Polyclonal Antibody has been used in 3 publications
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Hamster |
| Host Species | Goat |
| Conjugate | Unconjugated |
| Applications | Dot Blot,ELISA,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | 1 mg/mL |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000, Immunocytochemistry/Immunofluorescence, Dot Blot, Fluorophore-linked immunosorbent assay |
| Classification | Polyclonal |
| Immunogen | Armenian and Golden Syrian Hamster IgG, whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | This antibody was pre-adsorbed against Mouse and Rat Serum Proteins. No reaction was observed against Mouse or Rat serum proteins. Specificity was confirmed using ELISA at less than 1% cross reactivity. |
Goat anti-Feline IgG Fc Secondary Antibody, Alkaline Phosphatase, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Feline |
| Host Species | Goat |
| Conjugate | Alkaline Phosphatase (AP) |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Concentration | 1 mg/mL |
| Antigen | IgG Fc |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:500 - 1:2500, ELISA 1:1000 - 1:5000, Immunohistochemistry 1:200 - 1:1000 |
| Classification | Polyclonal |
| Immunogen | Cat IgG F(c) fragment |
| Primary or Secondary | Secondary |
| Test Specificity | No reaction was observed against Cat IgG F(ab). |
Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed), Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Human |
| Host Species | Goat |
| Conjugate | Unconjugated |
| Applications | ELISA,Flow Cytometry,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Epitope Tags, Immunology |
| Concentration | 0.5 mg/mL |
| Antigen | IgG F(ab')2 |
| Gene Symbols | IGHG1 |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:200 - 1:2000, Immunohistochemistry-Paraffin 1:200 - 1:2000 |
| Gene Alias | immunoglobulin heavy constant gamma 1 (G1m marker) |
| Gene ID (Entrez) | 3500 |
| Classification | Polyclonal |
| Immunogen | Human IgG-F(ab')2 Fragment |
| Primary or Secondary | Secondary |
| Test Specificity | By immunoelectrophoresis and ELISA this antibody reacts specifically with F(ab′)2 fragments of human IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with F(ab′)2 fragments of IgG from other species. |
Goat anti-Rat IgG Fc Secondary Antibody, DyLight 649 (Pre-adsorbed), Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Rat |
| Host Species | Goat |
| Conjugate | DyLight 649 |
| Applications | Flow Cytometry,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin) |
| Isotype | IgG |
| Concentration | 0.5 mg/mL |
| Antigen | IgG Fc |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Flow Cytometry 1:50 - 1:200, Immunohistochemistry 1:50 - 1:500, Immunocytochemistry/Immunofluorescence 1:50 - 1:500, Immunohistochemistry-Paraffin 1:50 - 1:500 |
| Classification | Polyclonal |
| Immunogen | Rat IgG-Fc Fragment |
| Primary or Secondary | Secondary |
| Test Specificity | Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight 649. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species. |
Donkey anti-Human IgG (H+L) Secondary Antibody, Alkaline Phosphatase, Novus Biologicals™
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Donkey Polyclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Human |
| Host Species | Donkey |
| Conjugate | Alkaline Phosphatase (AP) |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | 1 mg/mL |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:500 - 1:2500, ELISA 1:2000 - 1:10000, Immunohistochemistry 1:200 - 1:1000 |
| Classification | Polyclonal |
| Immunogen | Human IgG whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Human IgG and Human Serum. |
Goat anti-Guinea Pig IgG Fc Secondary Antibody, Alkaline Phosphatase, Novus Biologicals™
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Guinea Pig |
| Host Species | Goat |
| Conjugate | Alkaline Phosphatase (AP) |
| Applications | Dot Blot,ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Concentration | 1 mg/mL |
| Antigen | IgG Fc |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:500 - 1:2500, ELISA 1:2000 - 1:10000, Immunohistochemistry 1:200 - 1:1000, Dot Blot |
| Classification | Polyclonal |
| Immunogen | Guinea Pig IgG F(c) fragment |
| Primary or Secondary | Secondary |
| Test Specificity | No reaction was observed against Guinea Pig IgG F(ab). |
Mouse anti-Rat IgG1 Heavy Chain Secondary Antibody (MARG1-2), DyLight 488, Novus Biologicals™
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Mouse Monoclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Conjugate | DyLight 488 |
| Target Species | Rat |
| Host Species | Mouse |
| Applications | ELISA |
| Form | Purified |
| Isotype | IgG1 |
| Antigen | IgG1 Heavy Chain |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Formulation | 50mM Sodium Borate |
| Classification | Monoclonal |
| Immunogen | Rat IR27 IgG1 myeloma protein |
| Primary or Secondary | Secondary |
| Clone | MARG1-2 |