Secondary Antibodies
Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies
(44,180)
Mouse Secondary Antibodies
(14,490)
Rabbit Secondary Antibodies
(6,301)
Donkey Secondary Antibodies
(4,319)
Rat Secondary Antibodies
(1,234)
Sheep Secondary Antibodies
(701)
Chicken Secondary Antibodies
(403)
Bovine Secondary Antibodies
(257)
Pig Secondary Antibodies
(76)
Horse Secondary Antibodies
(74)
Alpaca Secondary Antibodies
(62)
Hamster Secondary Antibodies
(57)
Biosimilar Secondary Antibodies
(9)
Human Secondary Antibodies
(7)
Duck Secondary Antibodies
(3)
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Sheep anti-Rat IgG (H+L) Secondary Antibody, Rhodamine, Novus Biologicals™
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Sheep Polyclonal Antibody
| Content And Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Rat |
| Host Species | Sheep |
| Conjugate | Rhodamine |
| Applications | Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot |
| Form | Purified |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | 2.0 mg/mL |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Multi-step |
| Dilution | Western Blot 1:1000 - 1:10000, Flow Cytometry 1:500 - 1:2500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000 |
| Classification | Polyclonal |
| Immunogen | Rat IgG whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum, Rat IgG and Rat Serum. |
Donkey anti-Mouse IgG (H+L) Secondary Antibody, Alkaline Phosphatase, Novus Biologicals™
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Donkey Polyclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Mouse |
| Host Species | Donkey |
| Conjugate | Alkaline Phosphatase (AP) |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Form | Purified |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Immunogen affinity purified |
| Dilution | Western Blot 1:500 - 1:2500, ELISA 1:2000 - 1:10000, Immunohistochemistry 1:200 - 1:1000 |
| Classification | Polyclonal |
| Immunogen | Mouse IgG whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Mouse IgG, and Mouse Serum. |
Goat anti-Rat IgG (H+L) Secondary Antibody, Rhodamine, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Rat |
| Host Species | Goat |
| Conjugate | Rhodamine |
| Applications | Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot |
| Form | Purified |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Multi-step |
| Dilution | Western Blot 1:1000 - 1:10000, Flow Cytometry 1:500 - 1:2500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000 |
| Classification | Polyclonal |
| Immunogen | Rat IgG whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. |
Sheep anti-Rat IgG (H+L) Secondary Antibody, Biotin, Novus Biologicals™
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Sheep Polyclonal Antibody
| Content And Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Rat |
| Host Species | Sheep |
| Conjugate | Biotin |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Form | Purified |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Multi-step |
| Dilution | Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000 |
| Classification | Polyclonal |
| Immunogen | Rat IgG whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Sheep Serum, Rat IgG and Rat Serum. |
Goat anti-Guinea Pig IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Guinea Pig |
| Host Species | Goat |
| Conjugate | HRP |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Form | Purified |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | 1.0 mg/mL |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Multi-step |
| Dilution | Western Blot 1:1000 - 1:5000, ELISA 1:100000 - 1:200000, Immunohistochemistry 1:500 - 1:2500 |
| Classification | Polyclonal |
| Immunogen | Goat anti-Guinea Pig IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) was produced by repeated immunization with Guinea Pig IgG whole molecule in goat. |
| Reconstitution | Reconstitute with deionized water (or equivalent) to desired concetration. |
| Primary or Secondary | Secondary |
| Test Specificity | This antibody was pre-adsorbed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat, and Sheep Serum Proteins. No reaction was observed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. |
Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Texas Red, Novus Biologicals™
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Donkey Polyclonal Antibody
| Content And Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Rabbit |
| Host Species | Donkey |
| Conjugate | Texas Red |
| Applications | Dot Blot,Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Form | Purified |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Multi-step |
| Dilution | Western Blot, Flow Cytometry 1:1:10 - 1:1000, Immunohistochemistry 1:500 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Immunohistochemistry-Paraffin 1:500 - 1:1000, Dot Blot, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000 |
| Classification | Polyclonal |
| Immunogen | Rabbit IgG whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | Rabbit IgG H and L |
Goat anti-Feline IgM Heavy Chain Secondary Antibody, HRP, Novus Biologicals™
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Goat Polyclonal Antibody has been used in 4 publications
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Feline |
| Host Species | Goat |
| Conjugate | HRP |
| Applications | ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Isotype | IgG |
| Concentration | 1.0 mg/mL |
| Antigen | IgM Heavy Chain |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:2000 - 1:20000, ELISA 1:10000- 1:100000, Immunohistochemistry 1:200 - 1:2000, Immunocytochemistry/Immunofluorescence 1:200 - 1:2000, Immunohistochemistry-Paraffin 1:200-1:2000 |
| Gene Alias | Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain |
| Gene ID (Entrez) | 3507 |
| Classification | Polyclonal |
| Immunogen | Cat IgM |
| Primary or Secondary | Secondary |
| Test Specificity | By immunoelectrophoresis and ELISA this reacts specifically with cat IgM. This may cross react with IgM from other species. Antiserum was solid phase adsorbed to ensure class specificity. |
Donkey anti-Goat IgG (H+L) Secondary Antibody, Rhodamine, Novus Biologicals™
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Donkey Polyclonal Antibody
| Content And Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Goat |
| Host Species | Donkey |
| Conjugate | Rhodamine |
| Applications | Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence |
| Form | Purified |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Multi-step |
| Dilution | Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000 |
| Classification | Polyclonal |
| Immunogen | Goat IgG whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | Goat IgG (H and L) |
Goat anti-Chicken IgY Fc Secondary Antibody, Novus Biologicals™
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Goat Polyclonal Antibody has been used in 5 publications
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Chicken |
| Host Species | Goat |
| Conjugate | Unconjugated |
| Applications | ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Isotype | IgG |
| Concentration | 1.0 mg/mL |
| Antigen | IgY Fc |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:1000 - 1:30000, ELISA 1:1000 - 1:30000, Immunohistochemistry 1:200- 1:2000, Immunocytochemistry/Immunofluorescence 1:200- 1:2000, Immunohistochemistry-Paraffin 1:200- 1:2000 |
| Classification | Polyclonal |
| Immunogen | Chicken IgG (IgY)-Fc Fragment |
| Primary or Secondary | Secondary |
| Test Specificity | By immunoelectrophoresis and ELISA this reacts specifically with chicken IgG. This may cross react with IgG from other species. |
Goat anti-Chicken IgM Heavy Chain Secondary Antibody, Novus Biologicals™
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Goat Polyclonal Antibody has been used in 37 publications
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Chicken |
| Host Species | Goat |
| Conjugate | Unconjugated |
| Applications | ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Isotype | IgG |
| Concentration | 1.0 mg/mL |
| Antigen | IgM Heavy Chain |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:1000 - 1:30000, ELISA 1:1000-1:30000, Immunohistochemistry 1:200 - 1:2000, Immunocytochemistry/Immunofluorescence 1:200 - 1:2000, Immunohistochemistry-Paraffin 1:200 - 1:2000 |
| Gene Alias | Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain |
| Gene ID (Entrez) | 3507 |
| Classification | Polyclonal |
| Immunogen | Chicken IgM |
| Primary or Secondary | Secondary |
| Test Specificity | By immunoelectrophoresis and ELISA this reacts specifically with chicken IgM. This may cross react with IgM from other species. |
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Mouse IgG Fc Antibody, R&D Systems™
Mouse IgG Fc Antibody, R&D Systems™
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Goat Polyclonal Antibody has been used in 3 publications
| Content And Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 6 months, -20°C to -70°C under sterile conditions after reconstitution. |
|---|---|
| Target Species | Mouse |
| Host Species | Goat |
| Conjugate | Unconjugated |
| Applications | Not Available |
| Isotype | IgG |
| Antigen | IgG |
| Gene Symbols | IGHG1 |
| Regulatory Status | RUO |
| Purification Method | Antigen Affinity-purified |
| Dilution | Hidden |
| Gene Alias | Immunoglobulin G, ImmunoglobulinG |
| Gene ID (Entrez) | 3500 |
| Formulation | Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and Sodium Azide with Trehalose. with No Preservative |
| Immunogen | Mouse IgG |
| Classification | Polyclonal |
| Reconstitution | Reconstitute with sterile PBS. If 0.5 mL of PBS is used, the antibody concentration will be 0.2 mg/mL. |
| Primary or Secondary | Secondary |
| Test Specificity | This antibody reacts with heavy chains on mouse IgG, but not with light chains on mouse immunoglobulins. No reactivity is observed to non-immunoglobulin mouse serum proteins. |
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Human IgG (H+L) Antibody, R&D Systems™
Human IgG (H+L) Antibody, R&D Systems™
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Goat Polyclonal Antibody has been used in 2 publications
| Content And Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Target Species | Human |
| Host Species | Goat |
| Conjugate | Unconjugated |
| Applications | Not Available |
| Isotype | IgG |
| Antigen | IgG |
| Gene Symbols | IGHG1 |
| Regulatory Status | RUO |
| Purification Method | Antigen Affinity-purified |
| Dilution | Hidden |
| Gene Alias | Immunoglobulin G, ImmunoglobulinG |
| Gene ID (Entrez) | 3500 |
| Formulation | Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and Sodium Azide with Trehalose. with No Preservative |
| Immunogen | Human IgG |
| Classification | Polyclonal |
| Reconstitution | Reconstitute with sterile PBS. |
| Primary or Secondary | Secondary |
| Test Specificity | This antibody reacts with heavy chains on human IgG and with light chains on all human immunoglobulins. No reactivity is observed to non-immunoglobulin human, mouse, rabbit or bovine serum proteins by IEP. |
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody, R&D Systems™
Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody, R&D Systems™
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Donkey Polyclonal Antibody has been used in 2 publications
| Content And Storage | Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied. |
|---|---|
| Target Species | Sheep |
| Host Species | Donkey |
| Conjugate | PerCP |
| Applications | Flow Cytometry |
| Isotype | IgG |
| Antigen | IgG |
| Gene Symbols | IGHG1 |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Flow Cytometry 10 uL/10^6 cells |
| Gene Alias | Immunoglobulin G, ImmunoglobulinG |
| Gene ID (Entrez) | 3500 |
| Immunogen | Sheep IgG |
| Classification | Polyclonal |
| Primary or Secondary | Secondary |
| Test Specificity | This donkey anti-sheep IgG (H+L) antibody was purified by sheep IgG (H+L) affinity chromatography and absorbed against human IgG, and human, mouse, bovine and rabbit serum to eliminate cross-reactivity. |
Goat anti-Armenian Hamster IgG (H+L) Secondary Antibody, HRP, Novus Biologicals™
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Goat Polyclonal Antibody
| Antigen | IgG (H+L) |
|---|---|
| Regulatory Status | RUO |
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Target Species | Armenian Hamster |
| Host Species | Goat |
| Conjugate | HRP |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Form | Purified |
| Classification | Polyclonal |
| Immunogen | Armenian Hamster IgG whole molecule |
| Primary or Secondary | Secondary |
IgG secondary Sheep anti-Rabbit, Polyclonal, Secondary Antibody, MilliporeSigma™
Sheep Polyclonal Antibody