The induced rat liver S9 activation system was prepared from the livers of SD rats induced by the combination of sodium phenobarbital and β-naphthoflavone, containing cofactors required for metabolism. This system contains induced SD rat liver S9, Magnesium-potassium solution, D-Glucose 6-phosphate solution, Coenzyme II solution, and PBS. The protein concentration for this system is 3.5 mg/mL.