PCR Cloning Kits

16 – 30 of 121 results

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Invitrogen™ TOPO™ TA, Dual TOP10 (Supply Center Packaging)

For fast, efficient cloning and subsequent in vitro transcription/translation

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Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli

Efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System

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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing

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Specifications

Promoter	T7, T3
Format	Kit
Product Type	PCR Cloning Kit
Cloning Method	Blunt TOPO™
Bacterial or Yeast Strain	TOP10
For Use With (Application)	Chromatin Biology
Vector	Blunt DNA Cloning Vectors
Product Line	One Shot™
Cell Type	Chemically Competent

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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli

Provides a highly efficient, 5 minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing

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Invitrogen™ Bac-to-Bac™ C-His TOPO™ Cloning Kit

Bac-to-Bac™ C-His TOPO™ Cloning Kit

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Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit

Used in suspension-adapted cells for transient protein expression. Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.

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Invitrogen™ Ambion™ T4 RNA Ligase, cloned, 5 U/μL

Catalyzes formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor

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Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning

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Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO™ Vector and One Shot™ TOP10F' Chemically Competent E. coli

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Pricing & Availability
Specifications

Promoter	T7, SP6
Content And Storage	Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid. Store at -68 to -85°C.
Format	Kit
Product Type	Cloning Kit
Cloning Method	TOPO™-TA
Bacterial or Yeast Strain	TOP10F´
Vector	TOPO-TA Cloning Vectors
Product Line	One Shot™
Cell Type	Chemically Competent

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Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ TOP10 Electrocomp™ E. coli

Provides a highly efficient, 5 minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing

Pricing & Availability
Specifications

Promoter	T7, T3
Content And Storage	Box 1: • Topoisomerase I-activated pCR™4-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • T3, T7, M13 forward, and M13 reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C.
Format	Kit
Product Type	Cloning Kit
Cloning Method	TOPO™-TA
Bacterial or Yeast Strain	TOP10
For Use With (Application)	Chromatin Biology
Vector	TOPO-TA Cloning Vectors
Product Line	One Shot™

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Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10F' Chemically Competent E. coli

Provides quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into plasmid vector

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Specifications

Promoter	T7
Content And Storage	TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format	Kit
Product Type	Cloning Kit
Cloning Method	TA Cloning
Bacterial or Yeast Strain	TOP10F ́
For Use With (Application)	PCR Cloning
Vector	pCR2.1
Product Line	One Shot™
Cell Type	Chemically Competent E. coli

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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ MAX Efficiency DH5α-T1^R E. coli

Offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases

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Specifications

Promoter	SP6
Content And Storage	Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C.
Format	Kit
Product Type	PCR Cloning Kit
Cloning Method	Blunt TOPO™
Bacterial or Yeast Strain	DH5α
Vector	Blunt DNA Cloning Vectors
Product Line	One Shot™

Invitrogen™ RNase T1 (1 U/μL)

Invitrogen™ RNase T1, RNA-Grade cleaves 3' of single-stranded G residues and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites.

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Invitrogen™ TOPO™ TA Cloning™ Kit, with One Shot™ MAX Efficiency™ DH5α-T1^R E. coli

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Pricing & Availability
Specifications

Promoter	T7, SP6
Content And Storage	Box 1: • Topoisomerase I-activated pCR™2.1-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C.
Format	Kit
Product Type	Cloning Kit
Cloning Method	TOPO™-TA
Bacterial or Yeast Strain	DH5α
Vector	TOPO-TA Cloning Vectors
Product Line	One Shot™

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Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO™ Vector and One Shot™ TOP10 Electrocomp™ E. coli

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Pricing & Availability
Specifications

Promoter	T7, SP6
Content And Storage	Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid. Store at -68 to -85°C.
Format	Kit
Product Type	Cloning Kit
Cloning Method	TOPO™-TA
Bacterial or Yeast Strain	TOP10
Vector	TOPO-TA Cloning Vectors
Product Line	One Shot™

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