Transfection Reagents
System Biosciences LLC pMC.CMV-GFP-SV40PolyA Positive Control MinicirclePre-made Minicircle CMV-GFP-SV40PolyA (positive control) = 40 µg
Easily implement positive controls for your minicircle projects with pMC.CMV-GFP-SV40PolyA, a ready-to-transfect, pre-made, positive control minicircle. Maximize safety with Minicircle Technology—episomal expression free from foreign DNA Jump start your minicircle positive controls and troubleshooting with the ready-to-transfect pMC.CMV-GFP-SV40PolyA Pre-made Positive Control Minicircle (Cat.# MN601MC-1)., you can easily implement positive controls or troubleshoot your minicircle projects. This plasmid uses the strong CMV promoter to drive GFP expression so you can track transfection efficiencies and more with GFP imaging.
OriGene Lentiviral Packaging Kits, 5x10 reactions
Lenti-vpak packaging kit - packaging plasmids and transfection reagent, 5 packsComponents: Five kits each containing: 1 vial of packaging plasmids (60 ug) – lyophilized 1 vial of Turbofectin (400 ul) – liquidQuality Control: The reagent components have been successfully tested in transfecting HEK293 cells and producing pseudoviral particles for transduction.Stability: Stable for 1 year from receipt of products under proper storage and handling condition.Storage: Store in 4°C refrigerator upon arrival.Type: Lentiviral Packaging Kits
Cell Biologics Immortalized Mouse Primary Epidermal Keratinocytes
Immortalized Mouse Primary Epidermal Keratinocytes
STEMCELL Technologies Resazurin (Sodium Salt), 5 g
ReproRNA™-OKSGM is a single-stranded RNA replicon vector that contains five reprogramming factors: OCT4, KLF-4, SOX2, GLIS1, and c-MYC, as well as a puromycin-resistance gene (Figure 1). This RNA vector reprograms somatic cells, such as fibroblasts, into induced pluripotent stem (iPS) cells with high efficiency and only requires a single transfection step (Figure 2). When used together with ReproTeSR™ reprogramming medium, the generation of iPS cell colonies can be achieved under feeder-free conditions with superior colony morphology and similar reprogramming efficiency to feeder-based systems (Figures 3 - 4). ReproRNA™-derived iPS cell colonies also express markers of undifferentiated cells and retain a normal karyotype (Figures 5-6). Subsequently, iPS cells generated with ReproRNA™-OKSGM can be maintained in TeSR™ maintenance media and further differentiated into cells of all three germ layers (Figure 7).
Lonza Walkersville PRIMARY CELL 384-WELL NUCLEOFE
Primary Cell 384-well Nucleofector Kit 5010 (3840 reactions)
HORIZON DISCOVERY BIOSCIENCES LIMITED SIGLO RED TRANSFECTION INDICAT
NC3826711 SIGLO RED TRANSFECTION INDICAT
ATHENA ES NEW Expression Media Optimization Kit, ready-to-use, 15 mL each media
The Expression Media Optimization Kit™, enables researchers to determine the best media formulation that yields the highest amount of target protein. Since the production of a given recombinant protein can vary significantly depending on the type of medium employed, the EMSK™ provides sterile liquid aliquots of fifteen expression broths, including eleven proprietary formulations, as well as Animal Product Free(APF) versions of Miller and Lennox. The ready-to-use liquid kit contain 15 mL of each medium.
Lonza Walkersville SF Cell Line 4D-Nucleofector LV Kit XL, Catalog Number V4LC-2002, Reaction Volume Up to 20 mL, Kit Size 5 reactions, Processing Time Less than 10 min, Compatible System 4D-Nucleofector LV Unit
The Lonza SF Cell Line 4D-Nucleofector LV Kit XL is specifically designed for efficient, large-scale transfection of hard-to-transfect cell lines using the 4D-Nucleofector LV Unit. This closed-system nucleofection kit modifies up to 1 x 10^9 cells effectively, retaining cell viability. It allows variable reaction volumes and rapid processing, making it ideal for transient protein production and generating modified cell lines for various assays.
- Compatible system: 4D-Nucleofector LV Unit
- Reaction volume: Up to 20 mL
- Cell numbers supported: Up to 1 x 10^9 cells
- Processing time: Less than 10 minutes
- Kit size: 5 reactions
- Optimal cell lines: HEK293 suspension clones, CHO-S suspension clones, K-562, U-937
- Applications: Large scale transient protein production, generation of modified cell lines
- Key benefits: High transfection efficiencies, maintained cell functionality
- Storage conditions: 2 to 8 °C