Transfection Reagents
Dojindo Molecular Technologies Inc HilyMax (1ML x 1) is a high transfection efficiency reagent, and is the best reagent for cell signaling research.
HilyMax is a gene transfection reagent that forms a liposome to be used for highly efficient gene transfection to a wide variety of cells. HilyMax readily interacts with DNA because cationic liposomes (+) and anionic DNA (-) spontaneously form DNA liposome complexes. The overall charge of DNA-HilyMax complex is positive, such that the DNA-HilyMax complex is electrostatically bound on an anionic cell surface and introduces DNA into the cell by endocytosis. Since the serum in the growth medium does not interfere with the transfection using HilyMax, no exchange of the medium during the transfection is required. HilyMax does not contain biological components that might interfere with the transfection.
LAMDA BIOTECH INC Tranfection reagent: LipoFexin
LipoFexin is a lipid-based transfection reagent that forms complex with DNA or RNA, and transports the complex into cells. It works on a variety of adherent and suspension cell lines. It can be used for transfection of either DNA or RNA into eukaryotic cells in culture media with or without serum. LipoFexin has been compared to work at the similar way and efficiency as the Lipofectamine 2000 transfection reagent. LipoFexin has the following Special Features: • Superior transfection efficiency for a broad range of cell lines. • No requirement of removal of serum from culture medium. • No requirement for washing or changing of medium after transfection. • Low cytotoxicity.
Lonza Walkersville Basic Nucleofector Kit for Primary Mammalian Smooth Muscle Cells
In addition to various cell type-specific kits for primary smooth muscle cells, we also provide a cell group-specific Basic Nucleofector Kit. This kit is suited to determine the optimal transfection conditions for any mammalian smooth muscle cell type that lacks a cell type specific Optimized Protocol for the Nucleofector 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector Programs is tested in combination with the Basic Nucleofector Solution for smooth muscle cells. Benefits:Optimization can be performed within 1 experiment Detailed protocols guiding through the optimization procedure Optional result fine tuning with help of our Scientific Support TeamTransfection efficiencies up to 95%Viabilities up to 96%Applications:Kit suited for smooth muscle cells from different mammalian species and various organs Already tested for porcine vascular smooth muscle cells and coronary artery smmoth muscle cells (CASMC)
ATHENA ES NEW Expression Media Optimization Kit, ready-to-use, 15 mL each media
The Expression Media Optimization Kit™, enables researchers to determine the best media formulation that yields the highest amount of target protein. Since the production of a given recombinant protein can vary significantly depending on the type of medium employed, the EMSK™ provides sterile liquid aliquots of fifteen expression broths, including eleven proprietary formulations, as well as Animal Product Free(APF) versions of Miller and Lennox. The ready-to-use liquid kit contain 15 mL of each medium.
Lonza Walkersville Mouse Embryonic Fibroblast Nucleofector Kit 2
The Nucleofector 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. The Mouse Embryonic Fibroblast Nucleofector Kit 2 is one of our two kits suited for efficient transfectiion of primary mouse embyonic fibroblasts (MEF). Due to significant diversity between different MEF clones, we have developed two Nucleofector Kits (1 and 2) and an easy-to-use Nucleofector Starter Kit to determine which of the 2 kits is optimal for a specific MEF clone.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells -Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Mouse Embryonic Fibroblasts0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Expression Systems Transfection Medium, 100ml
Expression Systems Transfection Medium is designed to complement ESF 921 and ESF AF insect cell culture media for the cotransfection or transfection step in baculovirus vector production. Transfection Medium is a serum-free and animal-free formulation that facilitates vector DNA uptake by the insect host cells. Realize higher titers by using a formulation designed to work with ESF 921 and ESF AF.
• Serum-free, animal-free formulation works seamlessly with ESF 921 and ESF AF
• Appropriate for PEI or lipofection mediated transfection
• Optimized for use with Expres2 TR Transfection Reagent
• Increases virus vector production and transient expression
• Available in 20 mL or 100 mL
• In stock for immediate shipment
• To download Instructions for Use and/or COA please visit: www.expressionsystems.com
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