Transfection Reagents
Lonza Walkersville Mouse Neural Stem Cell Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary mouse neural stem cells (NSCs).The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Mouse Neural Stem Cells0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville SE Cell Line 4D-Nucleofector X Kit L
The SE Cell Line 4D-Nucleofector X Kit L is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesCells maintain functionality post transfectionConditions are transferable between 4D-Nucleofector System (including 4D-Nucleofector including LV Unit), 96-well Shuttle System and 384-well Nucleofector System Applications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow throughput applicationsContent:2.25 mL SE Cell Line Nucleofector Solution0.5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)24 x 100 µL Nucleocuvette Vessel,24 x Single use pipettes
Genesee Scientific Corporation TRANSFECTION REAGENT 0.1ML
0.1ml Reagent with 10ml Buffer0.1ml/UnitBrand: Polyplus-transfectionBest-in-Class DNA Transfection ReagentBased on Novel Cationic NanotechnologyReach Maximal Gene Expression in Hard-to-Transfect CellsExcellent Cell Viability & MorphologyHighly Efficient: Requires Minimal Reagent & DNAReady-to-Use ProtocolCost Effective
GeneCopoeia HIGH TITER PURIFIED AAV PRTCLS
AAPeB-EGFP-AV30-s20000-200-h / Quote #20250227-193810471high titer purified AAV particles for eGFP with GFAP promoter; AAV-PHP.eBserotype; 2mL (100uL x 2 vials, >10^13 GC/mL)
SignaGen Laboratories GENJET DNA REAGENT HUVEC 1ML
GenJet DNA In Vitro Tranfection Reagent for HUVEC was pre-optimized for most efficiently transfecting HUVEC.
LAMDA BIOTECH INC Tranfection reagent: LipoFexin
LipoFexin is a lipid-based transfection reagent that forms complex with DNA or RNA, and transports the complex into cells. It works on a variety of adherent and suspension cell lines. It can be used for transfection of either DNA or RNA into eukaryotic cells in culture media with or without serum. LipoFexin has been compared to work at the similar way and efficiency as the Lipofectamine 2000 transfection reagent. LipoFexin has the following Special Features: • Superior transfection efficiency for a broad range of cell lines. • No requirement of removal of serum from culture medium. • No requirement for washing or changing of medium after transfection. • Low cytotoxicity.
Dojindo Molecular Technologies Inc HilyMax (1ML x 1) is a high transfection efficiency reagent, and is the best reagent for cell signaling research.
HilyMax is a gene transfection reagent that forms a liposome to be used for highly efficient gene transfection to a wide variety of cells. HilyMax readily interacts with DNA because cationic liposomes (+) and anionic DNA (-) spontaneously form DNA liposome complexes. The overall charge of DNA-HilyMax complex is positive, such that the DNA-HilyMax complex is electrostatically bound on an anionic cell surface and introduces DNA into the cell by endocytosis. Since the serum in the growth medium does not interfere with the transfection using HilyMax, no exchange of the medium during the transfection is required. HilyMax does not contain biological components that might interfere with the transfection.
Expression Systems Transfection Medium, 100ml
Expression Systems Transfection Medium is designed to complement ESF 921 and ESF AF insect cell culture media for the cotransfection or transfection step in baculovirus vector production. Transfection Medium is a serum-free and animal-free formulation that facilitates vector DNA uptake by the insect host cells. Realize higher titers by using a formulation designed to work with ESF 921 and ESF AF.
• Serum-free, animal-free formulation works seamlessly with ESF 921 and ESF AF
• Appropriate for PEI or lipofection mediated transfection
• Optimized for use with Expres2 TR Transfection Reagent
• Increases virus vector production and transient expression
• Available in 20 mL or 100 mL
• In stock for immediate shipment
• To download Instructions for Use and/or COA please visit: www.expressionsystems.com