Transcription and Translation Reagents
Promega Transcend™ Nonradioactive Translation Detection Systems: IRNA
Non-Radioactive Translation Detection Systems for colorimetric or chemiluminescent detection of proteins synthesized in vitro. Biotinylated proteins are detected using Streptavidin-Alkaline Phosphatase or Streptavidin-HRP.
Promega Wheat Germ Extract
Wheat Germ Extract contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors) and is used for in vitro translation..
Promega TNT™ T7 Quick for PCR DNA
TNT T7 Quick for PCR DNA is a rapid, convenient, coupled transcription/translation system designed for optimum protein expression from PCR templates.
Promega 5X Transcription Optimized Buffer
Riboprobe System Buffers are qualified for use in applications where maintaining the integrity of RNA is critical. Product is quality tested to ensure the absence of detectable RNase activity.
Promega T7 RiboMAX™ Express RNAi System
The T7 RiboMAX Express RNAi System is an in vitro transcription system designed to produce milligram amounts of RNA suitable for use in RNAi assays.
Promega E. coli T7 S30 Extract System for Circular DNA
The E. coli T7 S30 Extract System for Circular DNA simplifies the transcription/translation of DNA sequences cloned in plasmid or lambda vectors containing a T7 promoter.
Promega Rabbit Reticulocyte Lysate, Untreated
Untreated Rabbit Reticulocyte Lysate contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors) but has not been treated with micrococcal nuclease.
Promega TNT™ Quick Coupled Transcription/Translation System
The TNT Quick Systems are convenient single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression.
Promega Rabbit Reticulocyte Lysate Systems, Nuclease Treated
Rabbit Reticulocyte Lysate Translation Systems are used in identification of mRNA species, characterization of their protein products and investigation of transcriptional and translational control.
Bio Rad Laboratories ISCRIPT CDNA SYNTHESIS KIT
iScript cDNA Synthesis Kit, 100 x 20microliter reactions, Includes: 5x iScript reaction mix, iScript enzyme mixture, nuclease-free water
Cosmo Bio Usa Inc PUREfrex 2.1, 250 µl
PUREfrex can be used to express proteins containing non-natural amino acids by exploiting Protein Express's CloverDirect™ line of tRNA reagents for site-directed protein functionalization. CloverDirect™ UAG or CGGG aminoacyl tRNAs are available charged with different non-natural amino acids that confer a range of functionality, including fluorescent-labeling, biotin-labeling, PEGylation, chemical crosslinking and photo-regulation. Targeting incorporation of specific non-natural amino acids is achieved by designing protein expression templates with UAG and/or CGGG codons at user-defined positions.
Trilink Bio Technologies Inc CleanCap Reagent AG, 1 umole
CleanCap Reagent AG is TriLink’s proprietary co-transcriptional capping reagent for in vitro transcription of 5’ capped mRNA resulting in a Cap 1 structure. CleanCap has shown to provide up to 98% capping efficiency.
Clontech Labs 3P Guide-it Long ssDNA Production System v2 | 50 rxns
The Guide-it Long ssDNA Production System v2 enables on-demand production of long single-stranded DNA (ssDNA) up to 5,000 nt in length for use as a repair template in knockin experiments involving CRISPR/Cas9 technology or other genome editing tools. This kit provides a simple and fast method for converting a dsDNA PCR product of any sequence into ssDNA via selective digestion of either the sense or the antisense strand. Each kit allows for preparation of 50 ssDNA templates.