Transcription and Translation Reagents
Cellscript Inc ScriptCap™ 2'-O-Methyltransferase Kit, 60 μg Cap 0-RNA per reaction, 25 reactions, 100 U/μl enzyme concentration, -20°C storage temperature, enhanced mRNA stability, up to 50% increase in vivo translation efficiency
The CELLSCRIPT ScriptCap™ 2'-O-Methyltransferase Kit provides an enzymatic solution for converting Cap 0-RNA to Cap 1-RNA, enhancing both stability and translation efficiency. This kit achieves nearly 100% capping efficiency and simplifies the workflow by allowing direct input of capped RNA into further reactions without cleanup steps.
- Converts Cap 0-RNA to Cap 1-RNA
- ~100% capping efficiency
- Up to 50% increase in mRNA translation efficiency
- Reaction capacity of 60 μg Cap 0-RNA per reaction
- 25 reactions per kit
- Storage at -20°C
- Enzyme concentration: 100 U/μl
- 30 min reaction incubation at 37°C
- No cleanup required before poly(A) tailing
- For research use only
Cellscript Inc ScriptGuard RNase Inhibitor, 40 U/μl, 10,000 units, 1:1 binding ratio, Kᵢ ~10⁻¹⁴ M, storage -20 °C, 50% glycerol solution
ScriptGuard RNase Inhibitor, 40 U/μl, 10,000 units, 1:1 binding ratio, Kᵢ ~10⁻¹⁴ M, storage -20 °C, 50% glycerol solution
Bellbrook Labs TRANSCREENER AMP2/GMP2 FP ASSAY
Transcreener AMP2/GMP2 FP Assay (1,000 Assay, 384 Well)
Enzo Life Sciences GW501516 (1mg). CAS: 317318-70-0
GW501516 is a specific agonist for PPARδ (EC50=1.1nM) with a 1'000-fold selectivity over the other human subtypes. Purity: ≥98% (HPLC). Appearance: White to off-white solid. Solubility: Soluble in DMSO (50mg/ml), dimethyl formamide (25mg/ml), 100% ethanol (12mg/ml) or methanol (50mg/ml); slightly soluble in water. Long Term Storage: +4°C.
Trilink Bio Technologies Inc ARCA, 1 umole
A key step in cellular mRNA processing is the addition of a 5’ cap structure, which is a 5'-5' triphosphate linkage between the 5' end of the RNA and a guanosine nucleotide. The cap is methylated enzymatically at the N-7 position of the guanosine to form mature mCAP. When preparing synthetic mRNA, the cap is often added prior to use in order to stabilize the mRNA and significantly enhance translation. Using a 4:1 mixture of a cap analog to GTP in transcription reactions will cap 80% of the resulting mRNAs. If mCAP (pre-methylated CAP) is used, only half of the asymmetrical mCAP will insert in the functional orientation. ARCA can only insert in the correct orientation, resulting in mRNAs that are translated twice as efficiently.
New England Biolabs, Inc. NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible) – 24 reactions
The NEBNext Multiplex Small RNA Library Prep Kit for Illumina (Index Primers 1-48) contains the adaptors, primers, enzymes and buffers required to convert small RNAs into indexed libraries for next-generation sequencing on the Illumina platform. The novel workflow has been optimized to minimize adaptor-dimers, while producing high-yield, high-diversity libraries. Please note that this kit, the NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible), enables use with your own barcode system. This kit contains adaptors but does not include multiplex primers.
BIOSEARCH TECHNOLOGIES INC (Lucigen) AmpliScribe T7-Flash Transcription Kit, 25 RxnsProduce the highest yields of RNA transcript in a fast, scalable 30-minute protocol.
The AmpliScribe™ T7-Flash™ Transcription Kit produces more RNA in 30 minutes than other in vitro transcription kits produce in two hours.
Active Motif Jurkat nuclear extract (TPA + CI stimulated)
Active Motif's Jurkat nuclear extract (TPA + CI stimulated) was prepared from cell cultures of the Jurkat human CD4(+) T cell lymphoblast-like cell line. The Jurkat cell line was established from the propagation of peripheral blood cells of a 14 year old boy with T cell leukemia. These cells are primarily used in research as a T cell model system, particularly in studies of T cell signaling, T cell leukemia, and response to viral infection. Jurkat cells are frequently used in HIV-related research to examine the mechanisms governing T cell-mediated immunity in response to HIV infection. Stimulation of these cells with phorbol esters, such as 12-O-tetradecanoylphorbol-13-acetate (TPA) and either lectins, such as phytohaemagglutinin (PHA) or […] For more information, please visit: https://www.activemotif.com/catalog/details/36013.
Trilink Bio Technologies Inc CleanCap Reagent AG
CleanCap Reagent AG is a 5X concentrated capping reagent for 5' end repair of DNA or RNA. It contains an enzyme mix to catalyze the addition of a cap structure to the 5' end of RNA or DNA. The cap consists of a guanine nucleotide connected to the 5' end via a 5'-5' triphosphate linkage. This cap protects the RNA or DNA from degradation and is required for efficient translation. The reagent also contains alkaline phosphatase to remove the 5' phosphate left by T4 PNK treatment. CleanCap Reagent AG is optimized for use in common molecular biology applications including library preparation for next generation sequencing.