Cell Based Assays
Invitrogen™ alamarBlue™ and alamarBlue™ HS Cell Viability Reagents
The alamarBlue cell viability reagents are ready-to-use resazurin-based solutions that function as cell health indicators by using the reducing power of living cells to quantitatively measure viability.
Invitrogen™ PrestoBlue™ and PrestoBlue™ HS Cell Viability Reagents
Monitor cell proliferation and viability with the resazurin-based PrestoBlue and PrestoBlue HS cell viability reagents. These nontoxic and cell permeable reagents quantitatively measure cell viability in as little as 10 minutes using absorbance- or fluorescence-based microplate readers.
Thermo Scientific™ Hoechst 33342 solution (20 mM), 5 mL
DAPI and Hoechst are classic popular nuclear counterstains for use in all fluorescent cell and tissue techniques. The blue fluorescence is vivid contrast to green, yellow, orange, red, far red and NIR fluorescent probes and labels.
Invitrogen™ CyQUANT™ LDH Cytotoxicity Assays
CyQUANT cytotoxicity assays measure cellular toxicity based on the release of cytosolic enzymes (LDH or G6PD) from damaged cells. These cytotoxicity assays utilize either colorimetric or fluorescence detection and allow continuous monitoring of cell death using microplate readers.
Invitrogen™ Calcein AM, Cell-permeant Green and Blue Dyes
Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells, the non-fluorescent Calcein AM is converted to green or blue fluorescent Calcein, after acetoxymethyl ester hydrolysis by intracellular esterases.
Invitrogen™ Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit
Sensitive, one-step assay to detect hydrogen peroxide (H2O2) or peroxidase activity
Invitrogen™ CellTrace™ Violet Cell Proliferation Kit, for flow cytometry
CellTrace™ Violet Cell Proliferation Kit, for flow cytometry
Molecular Probes™ LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation
The LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining, or prior to elimination of biohazardous materials using formaldehyde fixation.
Invitrogen™ MitoSOX™ Mitochondrial Superoxide Indicators, for live-cell imaging
MitoSOX superoxide indicators are novel fluorogenic dyes specifically targeted to mitochondria in live cells. Oxidation of the MitoSOX reagent by mitochondrial superoxide produces bright green or red fluorescence.
Invitrogen™ SYTOX™ Green Nucleic Acid Stain - 5 mM Solution in DMSO
Useful indicator of dead cells within a population
Molecular Probes™ MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)
MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)
Invitrogen™ CyQUANT™ MTT and XTT Cell Viability Assays
CyQUANT MTT and XTT Cell Viability assays are colorimetric microplate assays for accurate assessment of cell health, proliferation, and cytotoxicity. CyQUANT MTT is a well-established assay, while CyQUANT XTT offers improved performance and continuous measurement without cell lysis or solubilization.
Invitrogen™ LIVE/DEAD™ Cell Imaging Kit (488/570)
Sensitive two-color fluorescence cell viability assay optimized for FITC and Texas Red™ filters
Invitrogen™ CellROX™ Deep Red Reagent, for oxidative stress detection
CellROX™ Deep Red Reagent is a novel fluorogenic probe for measuring cellular oxidative stress in both live and fixed cell imaging, with absorption/emission maxima at ∼644/665 nm.
Invitrogen™ CellTracker™ Fluorescent Probes
CellTracker™ is a fluorescent dye well suited for monitoring cell movement or location. After loading into cells, the dye is well retained, allowing for multigenerational tracking of cellular movements.