Cell Based Assays
Eurofins Discoverx PathHunter eXpress P2RY4 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0263E2CP0L)
The PathHunter P2RY4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. UTP) based activation of P2RY4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY4 and EA-tagged β-Arrestin-2. Activation of P2RY4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ILEX LIFE SCIENCES LLC QuickZyme Sensitive Tissue Collagen Assay Kit, 96 Assays, 96-well plate format, colorimetric assay for low level collagen analysis (tissue, tissue homogenates samples)
QuickZyme Sensitive Tissue Collagen Assay Kit enables collagen analysis in small tissue samples with low collagen levels. The assay is based on quantitative colorimetric determination of hydroxyproline residues, obtained by acid hydrolysis of collagen. The assay allows quantitative measurement of all types of collagen, species independent. The only hydroxyproline-based assay which shows no matrix effect and no need for further dilution of hydrolysate. No drying of hydrolysate is needed, resulting in a fast and easy (96-well plate format) assay. Application: tissues with low collagen/hydroxyproline content (ex. liver). Sensitivity: 2.4 µM (0.3 µg/ml). Assay-time: < 2 hours. Collagen is a main component of extracellular matrix. Dysregulation in collagen production results in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Thus measurement of collagen production is important in many disease related studies.
Eurofins Discoverx PathHunter eXpress CCR2 Total GPCR Internalization Assay, 2pl Kit. (93-0679E3CP5M)
The PathHunter CCR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CCR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged CCR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CCR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mINSRa Functional Assay, 2-Plate Kit. (93-1015E3CP16M)
The PathHunter eXpress mINSRa Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated mINSRa dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged mINSRa and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ILEX LIFE SCIENCES LLC QuickZyme Sensitive Tissue Hydroxyproline Kit, 5 x 96 Assays Bulk Pack, 96-well plate format, colorimetric assay for low level collagen analysis (tissue, tissue homogenates samples)
QuickZyme Sensitive Tissue Hydroxyproline Assay Kit enables collagen analysis in small tissue samples with low collagen levels. Quantitative colorimetric determination of hydroxyproline residues, obtained by acid hydrolysis of collagen. The only hydroxyproline-based assay which shows no matrix effect and no need for further dilution of hydrolysate. No drying of hydrolysate is needed, resulting in a fast and easy (96-well plate format) assay. NOTE: Kit does not contain tubes and protocol for collagen hydrolysis. If you need these items please order the QuickZyme Sensitive Tissue Collagen Assay. Application: tissues with low collagen/hydroxyproline content (ex. liver). Sensitivity: 2.4 µM (0.3 µg/ml). Assay-time: < 2 hours. Collagen is a main component of extracellular matrix. Dysregulation in collagen production results in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Thus measurement of collagen production is important in many studies.
Eurofins Discoverx PathHunter eXpress DARC CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0548E2CP1L)
The PathHunter DARC eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of DARC GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged DARC and EA-tagged β-Arrestin-2. Activation of DARC stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress KDR/KDR Dimerization Assay, 2-Plate Kit. (93-0996E1CP0M)
The PathHunter KDR/KDR Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. VEGF-121) induced dimerization of KDR/KDR receptors. This product uses EFC technology. The cell line included overexpresses KDR target protein fused to PK alongside KDR target protein fused to EA. Upon ligand-induced dimerization of KDR/KDR, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CDC25A Degradation Assay, 2-Plate Kit. (93-0765E3CP0M)
The PathHunter eXpress CDC25A Degradation Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting the cell cycle checkpoint pathway. This kit enables detection of levels of CDC25a, allowing for assessment of CHK-1-activation-induced CDC25a degradation. This product uses EFC technology. The cell line included overexpresses CDC25a target protein fused to ProLabel. EA is contained in the detection reagent. Upon pathway-activation-induced degradation of CDC25a EFC signal is lost. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TACR2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0238E2CP0L)
The PathHunter TACR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. substance P) based activation of TACR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged TACR2 and EA-tagged β-Arrestin-2. Activation of TACR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ABclonal Technology HRP-conjugated B-Actin
This gene encodes one of six different actin proteins Actins are highly conserved proteins that are involved in cell motility structure integrity and intercellular signaling The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed Mutations in this gene cause Baraitser-Winter syndrome 1 which is characterized by intellectual disability with a distinctive facial appearance in human patients Numerous pseudogenes of this gene have been identified throughout the human genome
Eurofins Discoverx PathHunter eXpress CHRM1 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0859E3CP0L)
The PathHunter CHRM1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. acetylcholine) based activation of CHRM1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM1 and EA-tagged β-Arrestin-2. Activation of CHRM1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTGER2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0214E2CP2L)
The PathHunter PTGER2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of PTGER2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGER2 and EA-tagged β-Arrestin-2. Activation of PTGER2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter BDKRB2 Bioassay Kit, 2-Plate. (93-0189Y2-00113)
The PathHunter BDKRB2 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. bradykinin) based activation of BDKRB2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged BDKRB2 and EA-tagged β-Arrestin-2. Activation of BDKRB2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress EDNRB CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0593E2CP0L)
The PathHunter EDNRB eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. endothelin-3) based activation of EDNRB GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged EDNRB and EA-tagged β-Arrestin-2. Activation of EDNRB stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Insulin High Range Detection Kit, 100,000 Assay Points
HTRF Insulin High Range Detection Kit, 100,000 Assay Points