Signal Transduction Reagents and Kits
Promega ADP-Glo™ Kinase Assay + PLK1 Kinase Enzyme System
Recombinant full-length human PLK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. A member of the Polo-Like Kinase family, PLK1 localizes to centrosomes and undergoes dramatic subcellular relocation during the cell cycle.
Promega ADP-Glo™ Kinase Assay + MLK2 Kinase Enzyme System
Recombinant human mLK2 (1-446) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. mLK2 or mixed-lineage kinase 2 is a member of the serine/threonine kinase family, which activates MAPK8/JNK1 and MKK4/SEK1.
Promega BRK Kinase Enzyme System, 1mg
Recombinant full-length human BRK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. BRK is a member of the non-receptor tyrosine kinases (PTKs) that contains an amino terminal SH3 and SH2 domain as well as the catalytic domain.
Promega FLT3 (D835Y) Kinase Enzyme System, 10microg
Recombinant human FLT3 (D835Y) (571-993) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega HPK1 Kinase Enzyme System, 10microg
Recombinant human HPK1 (1-346) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. HPK1 or Hematopoietic progenitor kinase 1 is a hematopoietic cell-restricted member of the Ste20 serine/threonine kinase super family.
Promega CDK9/CyclinK Kinase Enzyme System, 10microg
Recombinant full-length human CDK9 and CyclinK proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CDK9/CyclinK is a member of the cyclin-dependent protein kinase (CDK) family.
Promega ADP-Glo™ Kinase Assay + RSK1 Kinase Enzyme System
Recombinant full-length human RSK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RSK1 is a member of the RSK (ribosomal S6 kinase) family that consists of growth factor-regulated serine/threonine kinases.
Promega ADP-Glo™ Kinase Assay + GRK5 Kinase Enzyme System
Recombinant full-length human GRK5 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. GRK5 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family.
Promega ADP-Glo Kinase Assay + p38 alpha Kinase Enzyme System, 1 each
Recombinant full-length human p38alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p38alpha (SAPK2A) is a member of the p38 MAPK family, which are activated by various environmental stresses and proinflammatory cytokines.
Promega ADP-Glo™ Kinase Assay + JNK3 Kinase Enzyme System
Recombinant full-length human JNK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. JNK3 is a member of the c-Jun N-terminal kinases (JNKs), which are part of the mitogen-activated protein (MAP) kinase family.
Promega ABL1 (E255K) Kinase Enzyme System, 10microg
Recombinant human ABL1 (E255K) (27-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega NEK2 Kinase Enzyme System, 10microg
Recombinant full-length human NEK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NEK2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans.
Promega STK33 Kinase Enzyme System, 10microg
Recombinant full-length human STK33 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. STK33 is a distant member of the CAMK group of serine/threonine kinases.
Promega ADP-Glo™ Kinase Assay + TGFβR1 Kinase Enzyme System
TGF beta R1 is a member of the TGF beta receptor subfamily and is a ser/thr protein kinase that forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF beta signal from the cell surface to the cytoplasm.
Promega ADP-Glo™ Kinase Assay + NEK3 Kinase Enzyme System
Recombinant full-length human NEK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NEK3 is a member of the NEK family of protein kinases that share high amino acid homology with NIMA (never in mitosis gene a).