Microspheres
SUPERTECHS INC TDT CONTROL CELL SUSPENSION
Supertechs Cat# 9500 is a fixed (paraformaldehyde) cell suspension containing human lymphoblastoid REH (TdT⁺) and RA-1 (TdT⁻) cells with stable TdT antigen. This cell suspension can be used as a control for TdT flow cytometric determinations. The control cell suspension contains a mixture of approximately 50% TdT⁺ and 50% TdT⁻ cells at a concentration of 2 x 10⁷ cells/mL (1.0 mL, ~20 tests) in a convenient dropper bottle. Our stabilized cell suspension has a shelf life of about 3 months after preparation when stored at 4°C. Control cells can be used for setting the threshold and gating in flow cytometry studies and for training technicians in performing and evaluating TdT flow cytometry experiments.
Sony Biotechnology Beads, Instrument Setup and Maintenance
The Automatic Setup Beads are designed to enable automated instrument setup and ensure consistent, day-to-day performance. They consist of two bead types, provided at ready-to-use concentrations. Each bead type supports precise alignment of the microfluidic chip with the lasers and facilitates accurate estimation of drop delay.
IBA LifeSciences MagStrep type3 Strep-Tactin beads (2ml) are magnetic beads (5% suspension) to purify biotinylated proteins
MagStrep “type3” Strep-Tactin beads have an agarose surface coated with the streptavidin variant Strep-Tactin and intended for purification of biotinylated proteins. Originally, Strep-Tactin was developed for binding and purification of Strep-tagII/Twin-Strep-tag proteins. Strep-Tactin still has the capability to bind biotin, which is used as specific competitor for elution of Strep-tagged proteins. The release is carried out under mild and physiological conditions by adding an excess of free biotin. This reversible binding to Strep-Tactin make them to a perfect tool for isolation of biotinylated proteins or complexes via pull-downs or co-immunoprecipitation (Co-IP) in batch format or high-throughput screenings.Due to the magnetic core no centrifugation step is required since the beads can be easily separated with a magnetic separator, reducing the risk of disturbing the pellet or loss of beads within the wash step to zero. 20 μl suspension correspond to 1 μl magnetic beads.
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Beckman Coulter L10 10Um Nist Traceab Filter Paper [Ea]
L10 Standard, nominal 10μm, Latex Particle (NIST Traceable), 1 x 15 mL Product No:C72516 NIST Traceable. Microsphere. For use with the Multisizer COULTER COUNTER particle analyzer
Dojindo Molecular Technologies Inc Cell Counting Kit-8 (CCK-8, 500 tests), cell viability detection in cell proliferation and cytotoxicity assays
DOJINDO is the original creator of WST dyes and Cell Counting Kit-8 (CCK-8). CCK-8 has been used by researchers all over the world for over 20 years. The reasons for this include 1) reliable and stable quality, 2) long-term stability (1 year: 0-5°C, 2 years: -20°C), 3) higher sensitivity than MTT, XTT, MTS, or WST-1, and 4) low toxicity to cells, a combination of features that have been improved over its long history and that no other company can imitate. CCK-8 enables sensitive colorimetric assays to determine cell viability in cell proliferation and cytotoxicity assays. The kit includes Dojindo's WST-8 which is reduced by dehydrogenase activity in cells to give a yellow-colored formazan dye. The amount of the dye, generated by the activity of dehydrogenases in cells, is directly proportional to the number of living cells. visit the supplier website at “https://dojindo.com/product/cell-counting-kit-8/”.
BD Cell Analysis 3P FC Beads BV605
BD™ FC beads are fluorescent beads that enable the software to determine spillover values (SOVs) for fluorescence compensation and calculate a fluorescence compensation matrix during setup of BD™ flow cytometers. The combination of BD™ CS&T beads and BD FC beads accurately sets the voltage and compensation for each of the channels of the instrument. BD FC beads can also be used to standardize multiple instruments.
Polysciences, Inc. Polybead Polystyrene Blue Dyed Microsphere 10.00m | 2mL
The dyed Polybeads are packaged in an economical 2.5% aqueous suspension and are available in a variety of sizes and colors. These blue dyed microspheres may be coated using adsorption, as described in Technical Data Sheet 238E. They also have amine groups that may be used for covalent coupling procedures using glutaraldehyde.
Spherotech STREPTAVIDIN COATED PS PARTICL
Streptavidin Polystyrene Particles, 0.5% w/v, 6.0-8.0 um, 5 mL
BANGS LABORATORIES INC Bangs Laboratories Quantum R-PE MESF Kit, Accurate Calibration for Flow Cytometry, 20 Tests
The Bangs Laboratories Quantum™ R-PE MESF kit offers a robust solution for standardizing fluorescence intensity measurements in flow cytometry. It ensures accurate calibration to MESF units, enabling consistent data across experiments. The kit includes distinct microsphere populations, mimicking labeled cells for assessing cellular expression and instrument performance. Ideal for fluorescence quantitation, this kit streamlines data interpretation with its QuickCal analysis template.
Spherotech Streptavidin Fluorescent Particle Kit, Yellow, Odd # peaks, 0.1% w/v, 2.5-2.9 um, 6x1 mL
Streptavidin Fluorescent Particle Kit, Yellow, Odd # peaks, 0.1% w/v, 2.5-2.9 um, 6x1 mL