Enzymes and Inhibitors
Thermo Scientific™ PagI (BspHI) (10 U/μL)
The PagI (BspHI) restriction enzyme recognizes T^CATGA sites and cuts best at 37°C in O buffer (Isoschizomers: BspHI, CciI, RcaI).
Invitrogen™ Anza™ 36 Eco31I
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Thermo Scientific™ FastDigest Pfl23II
Cut at C^GTACG sites with Thermo Scientific™ FastDigest™ BsiWI (Pfl23II), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PspLI).
MilliporeSigma™ Novagen™ Chicken Egg White Lysozyme Solution
For purification of both DNA, protein, and Gram-positive bacterial lysis
Invitrogen™ Anza™ 27 PvuI
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Thermo Scientific™ Alw44I (10 U/μL)
Cut at G^TGCAC sites with Alw44I (ApaLI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: ApaLI, VneI).
Thermo Scientific™ Eco130I (StyI) (10 U/μL)
Cut at C^CWWGG sites with Eco130I (StyI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: BssT1I, EcoT14I, ErhI, StyI).
MP Biomedicals™ Papain from Papaya Latex
Sulfhydryl protease from Carica papaya latex
MP Biomedicals™ Protocatechuate-3,4-dioxygenase, From Pseudomonas species
≥3u/mg solid, Off-white Powder. Used to study the metabolism of protocatechuate in Rhizobiaceae.
MilliporeSigma™ Calbiochem™ Phospholipase C, Bacillus cereus
Catalyzes hydrolysis of lecithins to 1,2-diglycerides and choline phosphate
Thermo Scientific Chemicals Elastase Substrate V
627.70 g/mol
MilliporeSigma™ Calbiochem™ Lysozyme, Chicken Egg White
Inhibits biological activity of lipopolysaccharides from periodontopathic bacteria
MP Biomedicals™ Arginase from Bovine Liver
Major degradative enzyme for arginine that converts arginine to ornithine
Thermo Scientific™ FaqI (BsmFI) (2 U/μL)
Cut at GGGAC(10/14)^ sites with FaqI (BsmFI) restriction enzyme, which performs best at 37°C in Tango (+SAM) buffer (Isoschizomers: BslFI, BsmFI).
Thermo Scientific™ EheI (SfoI) (10 U/μL)
Cut at GGC^GCC sites with EheI (SfoI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI).