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Thermo Scientific™ Endonuclease IV, E.coli (2 U/μL)

Catalog No. FEREN0591
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FEREN0591 100 units
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Catalog No. FEREN0591 Supplier Thermo Scientific™ Supplier No. EN0591
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Description

Cut oxidative damaged DNA at AP sites with this enzyme, which recognizes apurinic/apyrimidinic sites and cleaves the phosphodiester bond 5' to the lesion.

Endonuclease IV (Endo IV) recognizes apurinic/apyrimidinic (AP) sites of dsDNA and cleaves the phosphodiester bond 5' to the lesion generating a hydroxyl group at the 3'-terminus. The enzyme can also act as a 3'-diesterase that is able to release 3'-phosphoglycolate or 3'-phosphate from the damaged ends of dsDNA . Endo IV possesses also a 3'≥5' exonuclease activity. Its progression on substrates is sensitive to ionic strength, metal ions, EDTA, and reducing conditions. Substrates with 3'-recessed ends are preferred substrates for the 3'≥5' exonuclease activity .

The enzyme has no requirement for Mg2+ and is fully active in the presence of EDTA in moderate concentrations.

Applications
  • Studies of DNA damage and repair
  • Single cell electrophoresis (comet assay)
  • Antitumor drug research
  • DNA structure research
  • SNP analysis
TRUSTED_SUSTAINABILITY

Specifications

Concentration 2 U/μL
Enzyme Endonuclease
Quantity 100 units
Product Type Endonuclease IV

For Research Use Only. Not for use in diagnostic procedures.

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