Enzymes and Inhibitors
MilliporeSigma™ Novagen™ rLysozyme™ Solution
For lysis of Gram-negative bacteria
Thermo Scientific™ beta-Galactosidase Assay Reagent
Lyse cultured mammalian cells and measure beta-galactosidase activity using compatible M-PER Cell Lysis Reagent and colorimetric b-Gal Assay Reagent.
R&D Systems™ H-Met-Gly-Pro-AMC Fluorogenic Peptide Substrate
Substrate for Methionine Aminopeptidase 1, 1D and 2
R&D Systems™ Mca-KPLGL-Dpa-AR-NH2 Fluorogenic Peptide Substrate
Substrate for MMP-1, MMP-2, MMP-7, MMP-8, MMP-9, MMP-12, MMP-13, MMP-14, MMP-15, MMP-16, ADAM10, ADAM17/TACE, Cathepsin D, and Cathepsin E
Thermo Scientific™ SmaI, HC (50 U/μL)
Cut at CCC^GGG sites with SmaI restriction enzyme, which performs best at 30°C in Tango buffer (Isoschizomers: TspMI, XmaCI, XmaI).
Thermo Scientific™ Bst1107I (BstZ17I) (10 U/μL)
Cut at GTA^TAC sites with Bst1107I (BstZ17I) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: BssNAI, BstZ17I).
Thermo Scientific™ Alw26I (BsmAI) (10 U/μL)
Cut at GTCTC(1/5)^ sites with Alw26I (BsmAI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BsmAI, BstMAI).
Thermo Scientific™ Alw44I (10 U/μL)
Cut at G^TGCAC sites with Alw44I (ApaLI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: ApaLI, VneI).
Thermo Scientific™ Eco88I (AvaI) (10 U/μL)
Cut at C^YCGRG sites with Eco88I (AvaI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: Ama87I, AvaI, BmeT110I, BsiHKCI, BsoBIm).
BD AEC Substrate Kit
Substrate for peroxidase and is a widely used chromogen for immunohistochemical staining and immunoblotting
Thermo Scientific™ EheI (SfoI) (10 U/μL)
Cut at GGC^GCC sites with EheI (SfoI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI).
Thermo Scientific™ PagI (BspHI) (10 U/μL)
The PagI (BspHI) restriction enzyme recognizes T^CATGA sites and cuts best at 37°C in O buffer (Isoschizomers: BspHI, CciI, RcaI).
Thermo Scientific™ CseI (HgaI) (5 U/μL)
Cut at GACGC(5/10)^ sites with CseI (HgaI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: HgaI).
Thermo Scientific™ BseMI (BsrDI) (5 U/μL)
Cut at GCAATG(2/0)^ sites with BseMI (BsrDI) restriction enzyme, which performs best at 55°C in R buffer (Isoschizomers: Bse3DI, BsrDI).
R&D Systems™ 3'-Phosphoadenosine-5'-phosphosulfate
Substrate for sulfotransferase assay