Enzymes and Inhibitors
Thermo Scientific™ RNase H (5 U/μL)
Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand in RNA-DNA hybrids.
Thermo Scientific™ XhoI (10 U/μL)
Cut at C^TCGAG sites with XhoI restriction enzyme, which functions best at 37°C in R buffer (Isoschizomers: PaeR7I, Sfr274I, SlaI, StrI, TliI).
Thermo Scientific™ FastDigest SgsI
Cut at GG^CGCGCC sites with Thermo Scientific™ FastDigest™ AscI (SgsI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PalAI).
Invitrogen™ DQ™ Collagen, type IV From Human Placenta, Fluorescein Conjugate
Analog of the natural substrate
Invitrogen™ Nitro blue Tetrazolium Chloride (NBT)
Use to assess cell viability as function of redox potential
Thermo Scientific™ NdeI (10 U/μL)
The NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in O buffer (Isoschizomers: FauNDI).
Thermo Scientific™ EcoRII (10 U/μL)
Cut at ^CCWGG sites with EcoRII restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AjnI, BseBI, Bst2UI, BstNI, BstOI, Psp6I, PspGI).
Thermo Scientific™ MssI (PmeI) (5 U/μL)
The MssI (PmeI) restriction enzyme recognizes GTTT^AAAC sites and cuts best at 37°C in B buffer (Isoschizomers: PmeI).
Invitrogen™ CorrectASE™ Enzyme
CorrectASE™ enzyme removes mismatches caused by oligonuceotide synthesis errors, leading to a 3–10 fold reduction in mutations in your synthetic genes or fragments.
Thermo Scientific™ FastDigest Bsu15I
Cut at AT^CGAT sites with ClaI (Bsu15I) restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest™ Buffer.
Thermo Scientific™ FastDigest Eco91I
Cut at G^GTNACC sites with Thermo Scientific™ FastDigest™ Eco91I, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ Eco47I (AvaII) (10 U/μL)
The Eco47I (AvaII) restriction enzyme recognizes G^GWCC sites and cuts best at 37°C in R buffer (Isoschizomers: AvaII, Bme18I, SinI, VpaK11BI).
Invitrogen™ Anza™ T4 DNA Ligase Master Mix
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Thermo Scientific™ PaeI (SphI) (10 U/μL)
The PaeI (SphI) restriction enzyme recognizes GCATG^C sites and cuts best at 37°C in B buffer (Isoschizomers: BbuI, SphI).
Invitrogen™ DQ™ Green BSA - Special Packaging
Hydrolysis of substrate yields brightly fluorescent product