Enzymes and Inhibitors
Invitrogen™ TrueCut™ HiFi Cas9 Protein (1 μg/μL)
A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut™ HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.
Thermo Scientific™ Eco47III (AfeI) (10 U/μL)
The Eco47III (AfeI) restriction enzyme recognizes AGC^GCT sites and cuts best at 37°C in O buffer (Isoschizomers: AfeI, Aor51HI).
Thermo Scientific™ Terminal Deoxynucleotidyl Transferase (20 U/μL)
Terminal Deoxynucleotidyl Transferase (TdT) catalyzes the template-independent addition of deoxyribonucleotides to the 3'-OH terminus of DNA molecules.
Thermo Scientific™ Csp6I (CviQI) (10 U/μL)
Cut at G^TAC sites with Csp6I (CviQI) restriction enzyme, which performs best at 37°C in B buffer (Isoschizomers: AfaI, CviQI, RsaNI).
Thermo Scientific™ SalI (10 U/μL)
The SalI restriction enzyme recognizes G^TCGAC sites and cuts best at 37°C in O buffer.
Invitrogen™ C12FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside)
Produces a green fluorescence
Thermo Scientific™ beta-Galactosidase Assay Reagent
Lyse cultured mammalian cells and measure beta-galactosidase activity using compatible M-PER Cell Lysis Reagent and colorimetric b-Gal Assay Reagent.
Invitrogen™ DiFMUP (6,8-Difluoro-4-Methylumbelliferyl Phosphate)
Continuously assay acid phosphatases at low pH
Invitrogen™ Red/Green BODIPY™ PC-A2 (1-O-(6-BODIPY™ 558/568-Aminohexyl)-2-BODIPY™ FL C5-Sn-Glycero-3-Phosphocholine) (Ratiometric Phospholipase A2 Substrate)
Measure results in microplate assays or using flow cytometry
Invitrogen™ Rhodamine 110, bis-(p-Tosyl-L-Glycyl-L-Prolyl-L-Arginine Amide)
This Molecular Probes™ bisamide derivative of rhodamine 110 (R110) is a sensitive and selective substrate for assaying proteases in solution or inside living cells.