Modifying Enzyme Buffers
Revvity Health Sciences Inc HTRF Enzymatic Kinase Buffer (5X), 10 mL
HTRF Enzymatic Kinase Buffer (5X), 10 mL
Apexbio Technology LLC Phosphatase Inhibitor Cocktail 3 (100X in DMSO) 100x1ml
Numerous cellular processes are regulated by reversible protein phosphorylation with approximately 30% of proteins being phosphorylated during their lifespan Crude cell extracts contain various endogenous enzymes such as phosphatases and proteases which can degrade and alter proteins in the extracts Therefore the optimal method to increase the yield of intact proteins is to add inhibitors targeting these enzymes Phosphatase Inhibitor Cocktail 3 inhibits alkaline phosphatase L-isozymes and serine/threonine protein phosphatases such as protein phosphatase 1 and 2A (PP1 and PP2A) Phosphatase Inhibitor Cocktail 3 has been optimized and tested in cell extracts from various animal tissues Phosphatase Inhibitor Cocktail 3 can replace Phosphatase Inhibitor Cocktail 1 as the two cocktails have equivalent activities Phosphatase Inhibitor Cocktail 3 contains Cantharidin Bromotetramisole and Calyculin A This Phosphatase Inhibitor Cocktail is supplied as a ready-to-use solution in DMSO
Apexbio Technology LLC Phosphatase Inhibitor Cocktail 2 (100X in ddH2O) 100x1ml
Many cellular processes are regulated by reversible phosphorylation of proteins and approximately 30% of proteins may become phosphorylated during their lifetime Cellular crude extracts contain numerous endogenous enzymes such as phosphatases and proteases which can degrade and modify proteins present in the extract Addition of inhibitors for these enzymes is the optimal approach for maximizing intact protein yield Phosphatase Inhibitor Cocktail 2 inhibits tyrosine protein phosphatases acid phosphatases and alkaline phosphatases Phosphatase Inhibitor Cocktail 2 has been optimized and tested in cell extracts from multiple animal tissues This Phosphatase Inhibitor Cocktail 2 contains Sodium orthovanadate Sodium molybdate Sodium tartrate Imidazole and Sodium Fluoride It is supplied as a ready-to-use solution in ddH2O
Revvity Health Sciences Inc HTRF Phospho and Total protein blocking reagent, 6 ml
HTRF Phospho and Total protein blocking reagent, 6 ml
Boston Bioproducts Inc Kinase Extraction Buffer - 500ml
Kinase Extraction Buffer - 500ml (For Research Use and Further Manufacturing Only)
Revvity Health Sciences Inc HTRF Stimulation Buffer #1 (5X), 8 mL
HTRF Stimulation Buffer #1 (5X), 8 mL