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Reaction buffers for use in experiments involving modifying enzymes. Different buffer solutions create optimal reaction conditions for specific modifying enzymes, or facilitate multiple enzymatic reactions.
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Specifically digests the agarose polysaccharide core, which allows for gentle yet efficient recovery of DNA or RNA fragments from low melting point agarose.
Thermo Scientific enzymes for in vitro transcription are designed for efficient and reliable RNA synthesis. These enzymes cover the entire synthesis workflow from template preparation to post-transcriptional modifications, ensuring optimal performance and high yields of synthesized RNA.
Also available in 10 mg 25 mg 50 mg 100 mg 500 mg and bulk. Please contact Fisher for quotes. Casein kinase 1(delta)-IN-1 (WAY-643895) is an inhibitor of casein kinase 1(delta) (CK1(delta)) which inhibits CK1(delta). Casein kinase 1(delta)-IN-1 can be used in the study of neurodegenerative diseases such as Alzheimer's disease. purity: 99%
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Numerous cellular processes are regulated by reversible protein phosphorylation with approximately 30% of proteins being phosphorylated during their lifespan Crude cell extracts contain various endogenous enzymes such as phosphatases and proteases which can degrade and alter proteins in the extracts Therefore the optimal method to increase the yield of intact proteins is to add inhibitors targeting these enzymes Phosphatase Inhibitor Cocktail 3 inhibits alkaline phosphatase L-isozymes and serine/threonine protein phosphatases such as protein phosphatase 1 and 2A (PP1 and PP2A) Phosphatase Inhibitor Cocktail 3 has been optimized and tested in cell extracts from various animal tissues Phosphatase Inhibitor Cocktail 3 can replace Phosphatase Inhibitor Cocktail 1 as the two cocktails have equivalent activities Phosphatase Inhibitor Cocktail 3 contains Cantharidin Bromotetramisole and Calyculin A This Phosphatase Inhibitor Cocktail is supplied as a ready-to-use solution in DMSO
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Many cellular processes are regulated by reversible phosphorylation of proteins and approximately 30% of proteins may become phosphorylated during their lifetime Cellular crude extracts contain numerous endogenous enzymes such as phosphatases and proteases which can degrade and modify proteins present in the extract Addition of inhibitors for these enzymes is the optimal approach for maximizing intact protein yield Phosphatase Inhibitor Cocktail 2 inhibits tyrosine protein phosphatases acid phosphatases and alkaline phosphatases Phosphatase Inhibitor Cocktail 2 has been optimized and tested in cell extracts from multiple animal tissues This Phosphatase Inhibitor Cocktail 2 contains Sodium orthovanadate Sodium molybdate Sodium tartrate Imidazole and Sodium Fluoride It is supplied as a ready-to-use solution in ddH2O
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Casein Kinase 1 alpha Antibody (YA564) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting Casein Kinase 1 alpha. It is for research use only.
Host: Rabbit
Clonality: Recombinant, monoclonal
Species reactivity: Human, rat
Immunogen: Synthetic peptide corresponding to human Casein Kinase 1 alpha. The exact sequence is proprietary to MCE.
Sensitivity: Endogenous
Conjugation: Non-conjugated
Modification: Unmodified
Isotype: IgG
Appearance: Liquid
Formulation: Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide.
Storage and stability: Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping: Shipping with blue ice.
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